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题名: Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella
作者: Chen CongLin1; Sun XiaoYun2; Liao LanJie1; Luo ShaoXiang1; Li ZhouQuan1; Zhang XiaoHua1; Wang YaPing1; Guo QionLin1; Fang Qin2; Dai HePing1
通讯作者: Fang, Q (reprint author), Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.
关键词: grass carp ; grass carp reovirus (GCRV) ; IgM ; single-chain variable fragment (scFv) ; antigenicity
刊名: SCIENCE CHINA-LIFE SCIENCES
发表日期: 2013
DOI: 10.1007/s11427-012-4425-5
卷: 56, 期:1, 页:59-65
收录类别: SCI
文章类型: Article
部门归属: [Chen CongLin ; Liao LanJie ; Luo ShaoXiang ; Li ZhouQuan ; Zhang XiaoHua ; Wang YaPing ; Guo QionLin ; Dai HePing] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Sun XiaoYun ; Fang Qin] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434]
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
摘要: Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na < ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.
英文摘要: Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na < ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.
关键词[WOS]: TELEOST FISH ; PROTEIN ; AQUAREOVIRUS ; IDENTIFICATION ; EXPRESSION ; ISOTYPE ; VIRION ; VIRUS ; ASSAY ; VP5
语种: 英语
WOS记录号: WOS:000313447000008
ISSN号: 1674-7305
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19345
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China

Recommended Citation:
Chen, CL; Sun, XY; Liao, LJ; Luo, SX; Li, ZQ; Zhang, XH; Wang, YP; Guo, QL; Fang, Q; Dai, HP.Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella,SCIENCE CHINA-LIFE SCIENCES,2013,56(1):59-65
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