IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella
Chen CongLin1; Sun XiaoYun2; Liao LanJie1; Luo ShaoXiang1; Li ZhouQuan1; Zhang XiaoHua1; Wang YaPing1; Guo QionLin1; Fang Qin2; Dai HePing1; Fang, Q (reprint author), Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.
2013
Source PublicationSCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
Volume56Issue:1Pages:59-65
AbstractGrass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na < ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.; Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na < ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.
SubtypeArticle
KeywordGrass Carp Grass Carp Reovirus (Gcrv) Igm Single-chain Variable Fragment (Scfv) Antigenicity
Department[Chen CongLin ; Liao LanJie ; Luo ShaoXiang ; Li ZhouQuan ; Zhang XiaoHua ; Wang YaPing ; Guo QionLin ; Dai HePing] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Sun XiaoYun ; Fang Qin] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
DOI10.1007/s11427-012-4425-5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434] ; National Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434] ; National Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434] ; National Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434]
Indexed BySCI
Language英语
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS IDWOS:000313447000008
WOS KeywordTELEOST FISH ; PROTEIN ; AQUAREOVIRUS ; IDENTIFICATION ; EXPRESSION ; ISOTYPE ; VIRION ; VIRUS ; ASSAY ; VP5
Funding OrganizationNational Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434] ; National Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434] ; National Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434] ; National Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19345
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorFang, Q (reprint author), Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
Recommended Citation
GB/T 7714
Chen CongLin,Sun XiaoYun,Liao LanJie,et al. Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(1):59-65.
APA Chen CongLin.,Sun XiaoYun.,Liao LanJie.,Luo ShaoXiang.,Li ZhouQuan.,...&Fang, Q .(2013).Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella.SCIENCE CHINA-LIFE SCIENCES,56(1),59-65.
MLA Chen CongLin,et al."Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella".SCIENCE CHINA-LIFE SCIENCES 56.1(2013):59-65.
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