IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila
Cervantes, Marcella D.1; Hamilton, Eileen P.1; Xiong, Jie2; Lawson, Michael J.3; Yuan, Dongxia2; Hadjithomas, Michalis4; Miao, Wei2; Orias, Eduardo1; Cervantes, MD (reprint author), Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA.
2013-03-01
Source PublicationPLOS BIOLOGY
ISSN1545-7885
Volume11Issue:3Pages:e1001518
AbstractThe unicellular eukaryote Tetrahymena thermophila has seven mating types. Cells can mate only when they recognize cells of a different mating type as non-self. As a ciliate, Tetrahymena separates its germline and soma into two nuclei. During growth the somatic nucleus is responsible for all gene transcription while the germline nucleus remains silent. During mating, a new somatic nucleus is differentiated from a germline nucleus and mating type is decided by a stochastic process. We report here that the somatic mating type locus contains a pair of genes arranged head-to-head. Each gene encodes a mating type-specific segment and a transmembrane domain that is shared by all mating types. Somatic gene knockouts showed both genes are required for efficient non-self recognition and successful mating, as assessed by pair formation and progeny production. The germline mating type locus consists of a tandem array of incomplete gene pairs representing each potential mating type. During mating, a complete new gene pair is assembled at the somatic mating type locus; the incomplete genes of one gene pair are completed by joining to gene segments at each end of germline array. All other germline gene pairs are deleted in the process. These programmed DNA rearrangements make this a fascinating system of mating type determination.; The unicellular eukaryote Tetrahymena thermophila has seven mating types. Cells can mate only when they recognize cells of a different mating type as non-self. As a ciliate, Tetrahymena separates its germline and soma into two nuclei. During growth the somatic nucleus is responsible for all gene transcription while the germline nucleus remains silent. During mating, a new somatic nucleus is differentiated from a germline nucleus and mating type is decided by a stochastic process. We report here that the somatic mating type locus contains a pair of genes arranged head-to-head. Each gene encodes a mating type-specific segment and a transmembrane domain that is shared by all mating types. Somatic gene knockouts showed both genes are required for efficient non-self recognition and successful mating, as assessed by pair formation and progeny production. The germline mating type locus consists of a tandem array of incomplete gene pairs representing each potential mating type. During mating, a complete new gene pair is assembled at the somatic mating type locus; the incomplete genes of one gene pair are completed by joining to gene segments at each end of germline array. All other germline gene pairs are deleted in the process. These programmed DNA rearrangements make this a fascinating system of mating type determination.
SubtypeArticle
KeywordRna-seq Dna Rearrangements Genome Pyriformis Sequence Differentiation Transformation Elimination Inheritance Frequencies
Department[Cervantes, Marcella D. ; Hamilton, Eileen P. ; Orias, Eduardo] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA ; [Xiong, Jie ; Yuan, Dongxia ; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China ; [Lawson, Michael J.] Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA ; [Hadjithomas, Michalis] J Craig Venter Inst, Rockville, MD USA
DOI10.1371/journal.pbio.1001518
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship ; National Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship ; National Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship ; National Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000316794600018
WOS KeywordRNA-SEQ ; DNA REARRANGEMENTS ; GENOME ; PYRIFORMIS ; SEQUENCE ; DIFFERENTIATION ; TRANSFORMATION ; ELIMINATION ; INHERITANCE ; FREQUENCIES
Funding OrganizationNational Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship ; National Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship ; National Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship ; National Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship
Citation statistics
Cited Times:49[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19340
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorCervantes, MD (reprint author), Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA.
Affiliation1.Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China
3.Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA
4.J Craig Venter Inst, Rockville, MD USA
Recommended Citation
GB/T 7714
Cervantes, Marcella D.,Hamilton, Eileen P.,Xiong, Jie,et al. Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila[J]. PLOS BIOLOGY,2013,11(3):e1001518.
APA Cervantes, Marcella D..,Hamilton, Eileen P..,Xiong, Jie.,Lawson, Michael J..,Yuan, Dongxia.,...&Cervantes, MD .(2013).Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila.PLOS BIOLOGY,11(3),e1001518.
MLA Cervantes, Marcella D.,et al."Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila".PLOS BIOLOGY 11.3(2013):e1001518.
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