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题名: Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila
作者: Cervantes, Marcella D.1; Hamilton, Eileen P.1; Xiong, Jie2; Lawson, Michael J.3; Yuan, Dongxia2; Hadjithomas, Michalis4; Miao, Wei2; Orias, Eduardo1
通讯作者: Cervantes, MD (reprint author), Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA.
关键词: RNA-SEQ ; DNA REARRANGEMENTS ; GENOME ; PYRIFORMIS ; SEQUENCE ; DIFFERENTIATION ; TRANSFORMATION ; ELIMINATION ; INHERITANCE ; FREQUENCIES
刊名: PLOS BIOLOGY
发表日期: 2013-03-01
DOI: 10.1371/journal.pbio.1001518
卷: 11, 期:3, 页:e1001518
收录类别: SCI
文章类型: Article
部门归属: [Cervantes, Marcella D. ; Hamilton, Eileen P. ; Orias, Eduardo] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA ; [Xiong, Jie ; Yuan, Dongxia ; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China ; [Lawson, Michael J.] Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA ; [Hadjithomas, Michalis] J Craig Venter Inst, Rockville, MD USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship
类目[WOS]: Biochemistry & Molecular Biology ; Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
摘要: The unicellular eukaryote Tetrahymena thermophila has seven mating types. Cells can mate only when they recognize cells of a different mating type as non-self. As a ciliate, Tetrahymena separates its germline and soma into two nuclei. During growth the somatic nucleus is responsible for all gene transcription while the germline nucleus remains silent. During mating, a new somatic nucleus is differentiated from a germline nucleus and mating type is decided by a stochastic process. We report here that the somatic mating type locus contains a pair of genes arranged head-to-head. Each gene encodes a mating type-specific segment and a transmembrane domain that is shared by all mating types. Somatic gene knockouts showed both genes are required for efficient non-self recognition and successful mating, as assessed by pair formation and progeny production. The germline mating type locus consists of a tandem array of incomplete gene pairs representing each potential mating type. During mating, a complete new gene pair is assembled at the somatic mating type locus; the incomplete genes of one gene pair are completed by joining to gene segments at each end of germline array. All other germline gene pairs are deleted in the process. These programmed DNA rearrangements make this a fascinating system of mating type determination.
英文摘要: The unicellular eukaryote Tetrahymena thermophila has seven mating types. Cells can mate only when they recognize cells of a different mating type as non-self. As a ciliate, Tetrahymena separates its germline and soma into two nuclei. During growth the somatic nucleus is responsible for all gene transcription while the germline nucleus remains silent. During mating, a new somatic nucleus is differentiated from a germline nucleus and mating type is decided by a stochastic process. We report here that the somatic mating type locus contains a pair of genes arranged head-to-head. Each gene encodes a mating type-specific segment and a transmembrane domain that is shared by all mating types. Somatic gene knockouts showed both genes are required for efficient non-self recognition and successful mating, as assessed by pair formation and progeny production. The germline mating type locus consists of a tandem array of incomplete gene pairs representing each potential mating type. During mating, a complete new gene pair is assembled at the somatic mating type locus; the incomplete genes of one gene pair are completed by joining to gene segments at each end of germline array. All other germline gene pairs are deleted in the process. These programmed DNA rearrangements make this a fascinating system of mating type determination.
关键词[WOS]: RNA-SEQ ; DNA REARRANGEMENTS ; GENOME ; PYRIFORMIS ; SEQUENCE ; DIFFERENTIATION ; TRANSFORMATION ; ELIMINATION ; INHERITANCE ; FREQUENCIES
语种: 英语
WOS记录号: WOS:000316794600018
ISSN号: 1545-7885
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19340
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China
3.Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA
4.J Craig Venter Inst, Rockville, MD USA

Recommended Citation:
Cervantes, MD; Hamilton, EP; Xiong, J; Lawson, MJ; Yuan, DX; Hadjithomas, M; Miao, W; Orias, E.Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila,PLOS BIOLOGY,2013,11(3):e1001518
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