中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets
作者: Kim, Youngchang1, 2; Ye, Zi3; Joachimiak, Grazyna1, 2; Videau, Patrick4; Young, Jasmine4; Hurd, Kathryn4; Callahan, Sean M.4; Gornicki, Piotr5; Zhao, Jindong3; Haselkorn, Robert5; Joachimiak, Andrzej1, 2, 6
通讯作者: Haselkorn, R (reprint author), Univ Chicago, Dept Mol Genet & Cell Biol, 920 E 58Th St, Chicago, IL 60637 USA.
关键词: heterocyst differentiation ; mutagenesis ; X-ray crystallography
刊名: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
发表日期: 2013-05-07
DOI: 10.1073/pnas.1305971110
卷: 110, 期:19, 页:E1716-E1723
收录类别: SCI
文章类型: Article
部门归属: [Kim, Youngchang ; Joachimiak, Grazyna ; Joachimiak, Andrzej] Argonne Natl Lab, Midwest Ctr Struct Genom, Argonne, IL 60439 USA ; [Kim, Youngchang ; Joachimiak, Grazyna ; Joachimiak, Andrzej] Argonne Natl Lab, Struct Biol Ctr, Argonne, IL 60439 USA ; [Ye, Zi ; Zhao, Jindong] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; [Videau, Patrick ; Young, Jasmine ; Hurd, Kathryn ; Callahan, Sean M.] Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA ; [Gornicki, Piotr ; Haselkorn, Robert] Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA ; [Joachimiak, Andrzej] Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA
WOS标题词: Science & Technology
资助者: National Institutes of Health [GM094585]; National Science Foundation [MCB-1121346]; Ellison Medical Foundation; US Department of Energy, Office of Biological and Environmental Research [DE-AC02-06CH11357]; US Department of Energy Office of Science laboratory [DE-AC02-06CH11357]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and 29 bp at 2.50-, 3.00-, and 3.25-angstrom resolution, respectively. The highest-resolution structure shows fine details of specific protein-DNA interactions. The lower-resolution structures with longer DNA duplexes have similar interaction patterns and show how the flap domains interact with DNA in a sequence nonspecific fashion. Fifteen of 15 protein-DNA contacts predicted on the basis of the structure were confirmed by single amino acid mutations that abolished binding in vitro and complementation in vivo. A striking feature of the structure is the association of glutamate 71 from each subunit of the HetR dimer with three successive cytosines in each arm of the palindromic target, a feature that is conserved among all known heterocyst-forming cyanobacteria sequenced to date.
英文摘要: HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and 29 bp at 2.50-, 3.00-, and 3.25-angstrom resolution, respectively. The highest-resolution structure shows fine details of specific protein-DNA interactions. The lower-resolution structures with longer DNA duplexes have similar interaction patterns and show how the flap domains interact with DNA in a sequence nonspecific fashion. Fifteen of 15 protein-DNA contacts predicted on the basis of the structure were confirmed by single amino acid mutations that abolished binding in vitro and complementation in vivo. A striking feature of the structure is the association of glutamate 71 from each subunit of the HetR dimer with three successive cytosines in each arm of the palindromic target, a feature that is conserved among all known heterocyst-forming cyanobacteria sequenced to date.
关键词[WOS]: STRAIN PCC 7120 ; HETEROCYST PATTERN-FORMATION ; REPRESSOR-OPERATOR COMPLEX ; CRYSTAL-STRUCTURE ; AMINO-ACIDS ; SP PCC-7120 ; RECOGNITION ; PROTEIN ; DIFFERENTIATION ; BINDING
语种: 英语
WOS记录号: WOS:000319327700004
ISSN号: 0027-8424
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19337
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets.pdf(1951KB)----开放获取View 联系获取全文

作者单位: 1.Argonne Natl Lab, Midwest Ctr Struct Genom, Argonne, IL 60439 USA
2.Argonne Natl Lab, Struct Biol Ctr, Argonne, IL 60439 USA
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
4.Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA
5.Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA
6.Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA

Recommended Citation:
Kim, Y; Ye, Z; Joachimiak, G; Videau, P; Young, J; Hurd, K; Callahan, SM; Gornicki, P; Zhao, JD; Haselkorn, R; Joachimiak, A.Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets,PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(19):E1716-E1723
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Kim, Youngchang]'s Articles
[Ye, Zi]'s Articles
[Joachimiak, Grazyna]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Kim, Youngchang]‘s Articles
[Ye, Zi]‘s Articles
[Joachimiak, Grazyna]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace