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题名: Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals
作者: Wei, Ting1, 2; Zhang, Chao1, 2; Xu, Xin3; Hanna, Michelle4; Zhang, Xiaohua1; Wang, Yan1; Dai, Heping1; Xiao, Wei3, 4
通讯作者: Dai, HP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
关键词: Genotoxin ; Reporter ; Biosensor ; Yeast ; Sensitivity
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2013-06-15
DOI: 10.1016/j.bios.2013.01.029
卷: 44, 页:138-145
收录类别: SCI
文章类型: Article
部门归属: [Wei, Ting ; Zhang, Chao ; Zhang, Xiaohua ; Wang, Yan ; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; [Wei, Ting ; Zhang, Chao] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Xu, Xin ; Xiao, Wei] Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China ; [Hanna, Michelle ; Xiao, Wei] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者: Natural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009]
类目[WOS]: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
摘要: It has been well established that essentially all microbial mutagens are rodent carcinogens, yet current mutagen detection systems are limited by their detection sensitivity. Here we report the construction of a pair of hypersensitive biosensors by optimizing both reporters and the host strain. The resulting RNR3-yEGFP and HUG1-yEGFP reporters and the septuple yeast mutant in combination with the automated protocol not only remarkably enhance the detection sensitivity, but also allow a high throughput screen of environmental genotoxins. This system is deemed much more sensitive than similar yeast and bacterium-based tests for all selected chemicals examined in this study. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要: It has been well established that essentially all microbial mutagens are rodent carcinogens, yet current mutagen detection systems are limited by their detection sensitivity. Here we report the construction of a pair of hypersensitive biosensors by optimizing both reporters and the host strain. The resulting RNR3-yEGFP and HUG1-yEGFP reporters and the septuple yeast mutant in combination with the automated protocol not only remarkably enhance the detection sensitivity, but also allow a high throughput screen of environmental genotoxins. This system is deemed much more sensitive than similar yeast and bacterium-based tests for all selected chemicals examined in this study. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: PROMOTER-REPORTER CONSTRUCT ; GREEN FLUORESCENT PROTEIN ; INDUCED GENE-EXPRESSION ; DNA-DAMAGE ; SACCHAROMYCES-CEREVISIAE ; ESCHERICHIA-COLI ; TESTING SYSTEM ; ENHANCES SENSITIVITY ; CWP GENES ; ASSAY
语种: 英语
WOS记录号: WOS:000317150700024
ISSN号: 0956-5663
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19336
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China
4.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada

Recommended Citation:
Wei, T; Zhang, C; Xu, X; Hanna, M; Zhang, XH; Wang, Y; Dai, HP; Xiao, W.Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals,BIOSENSORS & BIOELECTRONICS,2013,44():138-145
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