IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals
Wei, Ting1,2; Zhang, Chao1,2; Xu, Xin3; Hanna, Michelle4; Zhang, Xiaohua1; Wang, Yan1; Dai, Heping1; Xiao, Wei3,4; Dai, HP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
2013-06-15
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume44Pages:138-145
AbstractIt has been well established that essentially all microbial mutagens are rodent carcinogens, yet current mutagen detection systems are limited by their detection sensitivity. Here we report the construction of a pair of hypersensitive biosensors by optimizing both reporters and the host strain. The resulting RNR3-yEGFP and HUG1-yEGFP reporters and the septuple yeast mutant in combination with the automated protocol not only remarkably enhance the detection sensitivity, but also allow a high throughput screen of environmental genotoxins. This system is deemed much more sensitive than similar yeast and bacterium-based tests for all selected chemicals examined in this study. (C) 2013 Elsevier B.V. All rights reserved.; It has been well established that essentially all microbial mutagens are rodent carcinogens, yet current mutagen detection systems are limited by their detection sensitivity. Here we report the construction of a pair of hypersensitive biosensors by optimizing both reporters and the host strain. The resulting RNR3-yEGFP and HUG1-yEGFP reporters and the septuple yeast mutant in combination with the automated protocol not only remarkably enhance the detection sensitivity, but also allow a high throughput screen of environmental genotoxins. This system is deemed much more sensitive than similar yeast and bacterium-based tests for all selected chemicals examined in this study. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordGenotoxin Reporter Biosensor Yeast Sensitivity
Department[Wei, Ting ; Zhang, Chao ; Zhang, Xiaohua ; Wang, Yan ; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; [Wei, Ting ; Zhang, Chao] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Xu, Xin ; Xiao, Wei] Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China ; [Hanna, Michelle ; Xiao, Wei] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
DOI10.1016/j.bios.2013.01.029
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationNatural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009] ; Natural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009] ; Natural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009] ; Natural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009]
Indexed BySCI
Language英语
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000317150700024
WOS KeywordPROMOTER-REPORTER CONSTRUCT ; GREEN FLUORESCENT PROTEIN ; INDUCED GENE-EXPRESSION ; DNA-DAMAGE ; SACCHAROMYCES-CEREVISIAE ; ESCHERICHIA-COLI ; TESTING SYSTEM ; ENHANCES SENSITIVITY ; CWP GENES ; ASSAY
Funding OrganizationNatural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009] ; Natural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009] ; Natural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009] ; Natural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009]
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19336
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorDai, HP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China
4.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
Recommended Citation
GB/T 7714
Wei, Ting,Zhang, Chao,Xu, Xin,et al. Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals[J]. BIOSENSORS & BIOELECTRONICS,2013,44:138-145.
APA Wei, Ting.,Zhang, Chao.,Xu, Xin.,Hanna, Michelle.,Zhang, Xiaohua.,...&Dai, HP .(2013).Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals.BIOSENSORS & BIOELECTRONICS,44,138-145.
MLA Wei, Ting,et al."Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals".BIOSENSORS & BIOELECTRONICS 44(2013):138-145.
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