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Effects of acute exposure to polybrominated diphenyl ethers on retinoid signaling in zebrafish larvae
Xu, Tao1; Chen, Lianguo2; Hu, Chenyan2; Zhou, Bingsheng2; Chen, LG (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013
Source PublicationENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
ISSN1382-6689
Volume35Issue:1Pages:13-20
AbstractThe objectives of the present study were to investigate the effects of acute exposure to PBDEs on retinoid signaling in fish. Zebrafish embryos (2 h post-fertilization, hpf) were exposed to DE-71 (0, 31.0, 68.7, and 227.6 mu g/L) until 120 hpf. Retinoid profiles showed the content of retinal and retinoic acid was reduced significantly. While a significant up-regulation was observed in the transcription of retinal dehydrogenase (raldh2), the transcription of retinol binding protein (rbp1a), retinol dehydrogenase (rdh1), cellular retinoic acid binding protein.(crabp1a and crabp2a) and retinoic acid receptor subunit (raraa) were down-regulated significantly, indicating disruption of retinoid signaling. However, the transcriptions of five opsin genes (zfrho, zfuv, zfred, zfblue, and zfgr1) were up-regulated. Furthermore, whole mount immunostaining and western blotting demonstrated increased rhodopsin protein expression in the exposure groups. Overall, the results indicated that acute exposure to PBDEs could disturb retinoid signaling and may impact on eye development of zebrafish larvae. (C) 2012 Elsevier B.V. All rights reserved.; The objectives of the present study were to investigate the effects of acute exposure to PBDEs on retinoid signaling in fish. Zebrafish embryos (2 h post-fertilization, hpf) were exposed to DE-71 (0, 31.0, 68.7, and 227.6 mu g/L) until 120 hpf. Retinoid profiles showed the content of retinal and retinoic acid was reduced significantly. While a significant up-regulation was observed in the transcription of retinal dehydrogenase (raldh2), the transcription of retinol binding protein (rbp1a), retinol dehydrogenase (rdh1), cellular retinoic acid binding protein.(crabp1a and crabp2a) and retinoic acid receptor subunit (raraa) were down-regulated significantly, indicating disruption of retinoid signaling. However, the transcriptions of five opsin genes (zfrho, zfuv, zfred, zfblue, and zfgr1) were up-regulated. Furthermore, whole mount immunostaining and western blotting demonstrated increased rhodopsin protein expression in the exposure groups. Overall, the results indicated that acute exposure to PBDEs could disturb retinoid signaling and may impact on eye development of zebrafish larvae. (C) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordPolybrominated Diphenyl Ethers Retinoid Signaling Retinoic Acid Rhodopsin Zebrafish Embryos
Department[Xu, Tao] Huazhong Univ Sci & Technol, Puai Hosp, Wuhan 430033, Peoples R China ; [Chen, Lianguo ; Hu, Chenyan ; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.etap.2012.10.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB17] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB17] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB17] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB17]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
WOS SubjectEnvironmental Sciences ; Pharmacology & Pharmacy ; Toxicology
WOS IDWOS:000314262300002
WOS KeywordBROMINATED FLAME RETARDANTS ; VITAMIN-A ; GENE-EXPRESSION ; MATERNAL TRANSFER ; PHOTORECEPTOR DEVELOPMENT ; ENVIRONMENTAL-POLLUTANTS ; EMBRYONIC-DEVELOPMENT ; THYROID-HORMONE ; DANIO-RERIO ; ACID
Funding OrganizationNational Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB17] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB17] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB17] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB17]
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19330
Collection水环境工程研究中心_期刊论文
Corresponding AuthorChen, LG (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Huazhong Univ Sci & Technol, Puai Hosp, Wuhan 430033, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Xu, Tao,Chen, Lianguo,Hu, Chenyan,et al. Effects of acute exposure to polybrominated diphenyl ethers on retinoid signaling in zebrafish larvae[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2013,35(1):13-20.
APA Xu, Tao,Chen, Lianguo,Hu, Chenyan,Zhou, Bingsheng,&Chen, LG .(2013).Effects of acute exposure to polybrominated diphenyl ethers on retinoid signaling in zebrafish larvae.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,35(1),13-20.
MLA Xu, Tao,et al."Effects of acute exposure to polybrominated diphenyl ethers on retinoid signaling in zebrafish larvae".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 35.1(2013):13-20.
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