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题名: Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic-pituitary-thyroid axis
作者: Wang, Qiangwei1, 2; Liang, Kang3; Liu, Jingfu3; Yang, Lihua1; Guo, Yongyong1; Liu, Chunsheng4, 5; Zhou, Bingsheng1
通讯作者: Liu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210046, Jiangsu, Peoples R China.
关键词: TDCPP ; Thyroid endocrine disruption ; Organophosphorus flame retardants ; HPT axis
刊名: AQUATIC TOXICOLOGY
发表日期: 2013-01-15
DOI: 10.1016/j.aquatox.2012.11.009
卷: 126, 期:-, 页:207-213
收录类别: SCI
文章类型: Article
部门归属: [Wang, Qiangwei; Yang, Lihua; Guo, Yongyong; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Wang, Qiangwei] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Liang, Kang; Liu, Jingfu] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China; [Liu, Chunsheng] Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210046, Jiangsu, Peoples R China; [Liu, Chunsheng] Nanjing Univ, Sch Environm, Nanjing 210046, Jiangsu, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [21207063, 20890113]; Natural Science Foundation of Jiangsu Province, China [BK2011032]
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) has been frequently detected in the environment and in various biota, including fish, and has been implicated in disruption of the thyroid endocrine system. In the present study, zebrafish (Danio rerio) embryos were exposed to different concentrations of TDCPP (10, 50, 100, 300 and 600 mu g/L) from 2 h post-fertilization (hpf) to 144 hpf. Developmental endpoints, and whole-body concentrations of thyroid hormones and transcriptional profiles of genes involved in the hypothalamic-pituitary-thyroid (HPT) axis were examined. Exposure to TDCPP caused a dose-dependent developmental toxicity, including decreased body weight, reduced hatching, survival and heartbeat rates, and increased malformation (spinal curvature). Treatment with the positive control chemical 3,3',5-triiodo-L-thyronine (T3) significantly decreased whole-body thyroxin (T4) concentrations, increased whole-body T3 concentrations, and upregulated mRNA expression involved in the HPT axis as a compensatory mechanism. These results suggested that the HPT axis in 144-hpf zebrafish larvae was responsive to chemical exposure and could be used to evaluate the effects of chemicals on the thyroid endocrine system. TDCPP exposure significantly decreased whole-body T4 concentrations and increased whole-body T3 concentrations, indicating thyroid endocrine disruption. The upregulation of genes related to thyroid hormone metabolism (dio1 and ugt1ab) might be responsible for decreased T4 concentrations. Treatment with TDCPP also significantly increased transcription of genes involved in thyroid hormone synthesis (tsh beta, slc5a5 and tg) and thyroid development (hhex, nkx2.1 and pax8) as a compensatory mechanism for decreased T4 concentrations. Taken together, these results suggest that TDCPP alters the transcription of genes involved in the HPT axis and changes whole-body concentrations of thyroid hormones in zebrafish embryos/larvae, thus causing an endocrine disruption of the thyroid system. (c) 2012 Elsevier B.V. All rights reserved.
英文摘要: Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) has been frequently detected in the environment and in various biota, including fish, and has been implicated in disruption of the thyroid endocrine system. In the present study, zebrafish (Danio rerio) embryos were exposed to different concentrations of TDCPP (10, 50, 100, 300 and 600 mu g/L) from 2 h post-fertilization (hpf) to 144 hpf. Developmental endpoints, and whole-body concentrations of thyroid hormones and transcriptional profiles of genes involved in the hypothalamic-pituitary-thyroid (HPT) axis were examined. Exposure to TDCPP caused a dose-dependent developmental toxicity, including decreased body weight, reduced hatching, survival and heartbeat rates, and increased malformation (spinal curvature). Treatment with the positive control chemical 3,3',5-triiodo-L-thyronine (T3) significantly decreased whole-body thyroxin (T4) concentrations, increased whole-body T3 concentrations, and upregulated mRNA expression involved in the HPT axis as a compensatory mechanism. These results suggested that the HPT axis in 144-hpf zebrafish larvae was responsive to chemical exposure and could be used to evaluate the effects of chemicals on the thyroid endocrine system. TDCPP exposure significantly decreased whole-body T4 concentrations and increased whole-body T3 concentrations, indicating thyroid endocrine disruption. The upregulation of genes related to thyroid hormone metabolism (dio1 and ugt1ab) might be responsible for decreased T4 concentrations. Treatment with TDCPP also significantly increased transcription of genes involved in thyroid hormone synthesis (tsh beta, slc5a5 and tg) and thyroid development (hhex, nkx2.1 and pax8) as a compensatory mechanism for decreased T4 concentrations. Taken together, these results suggest that TDCPP alters the transcription of genes involved in the HPT axis and changes whole-body concentrations of thyroid hormones in zebrafish embryos/larvae, thus causing an endocrine disruption of the thyroid system. (c) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: UDP-GLUCURONOSYLTRANSFERASE INDUCERS ; ORGANOPHOSPHORUS FLAME RETARDANTS ; POLYBROMINATED DIPHENYL ETHERS ; ENDOCRINE DISRUPTION ; EXPRESSION ; RATS ; METABOLISM ; CELLS ; GLAND ; PLASTICIZERS
语种: 英语
项目资助者: National Natural Science Foundation of China(21207063 ; Natural Science Foundation of Jiangsu Province, China(BK2011032) ; 20890113)
WOS记录号: WOS:000315125600022
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19329
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
4.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210046, Jiangsu, Peoples R China
5.Nanjing Univ, Sch Environm, Nanjing 210046, Jiangsu, Peoples R China
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