IHB OpenIR  > 水环境工程研究中心  > 期刊论文
Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic-pituitary-thyroid axis
Wang, Qiangwei1,2; Liang, Kang3; Liu, Jingfu3; Yang, Lihua1; Guo, Yongyong1; Liu, Chunsheng4,5; Zhou, Bingsheng1; Liu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210046, Jiangsu, Peoples R China.
2013-01-15
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume126Issue:-Pages:207-213
AbstractTris(1,3-dichloro-2-propyl) phosphate (TDCPP) has been frequently detected in the environment and in various biota, including fish, and has been implicated in disruption of the thyroid endocrine system. In the present study, zebrafish (Danio rerio) embryos were exposed to different concentrations of TDCPP (10, 50, 100, 300 and 600 mu g/L) from 2 h post-fertilization (hpf) to 144 hpf. Developmental endpoints, and whole-body concentrations of thyroid hormones and transcriptional profiles of genes involved in the hypothalamic-pituitary-thyroid (HPT) axis were examined. Exposure to TDCPP caused a dose-dependent developmental toxicity, including decreased body weight, reduced hatching, survival and heartbeat rates, and increased malformation (spinal curvature). Treatment with the positive control chemical 3,3',5-triiodo-L-thyronine (T3) significantly decreased whole-body thyroxin (T4) concentrations, increased whole-body T3 concentrations, and upregulated mRNA expression involved in the HPT axis as a compensatory mechanism. These results suggested that the HPT axis in 144-hpf zebrafish larvae was responsive to chemical exposure and could be used to evaluate the effects of chemicals on the thyroid endocrine system. TDCPP exposure significantly decreased whole-body T4 concentrations and increased whole-body T3 concentrations, indicating thyroid endocrine disruption. The upregulation of genes related to thyroid hormone metabolism (dio1 and ugt1ab) might be responsible for decreased T4 concentrations. Treatment with TDCPP also significantly increased transcription of genes involved in thyroid hormone synthesis (tsh beta, slc5a5 and tg) and thyroid development (hhex, nkx2.1 and pax8) as a compensatory mechanism for decreased T4 concentrations. Taken together, these results suggest that TDCPP alters the transcription of genes involved in the HPT axis and changes whole-body concentrations of thyroid hormones in zebrafish embryos/larvae, thus causing an endocrine disruption of the thyroid system. (c) 2012 Elsevier B.V. All rights reserved.; Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) has been frequently detected in the environment and in various biota, including fish, and has been implicated in disruption of the thyroid endocrine system. In the present study, zebrafish (Danio rerio) embryos were exposed to different concentrations of TDCPP (10, 50, 100, 300 and 600 mu g/L) from 2 h post-fertilization (hpf) to 144 hpf. Developmental endpoints, and whole-body concentrations of thyroid hormones and transcriptional profiles of genes involved in the hypothalamic-pituitary-thyroid (HPT) axis were examined. Exposure to TDCPP caused a dose-dependent developmental toxicity, including decreased body weight, reduced hatching, survival and heartbeat rates, and increased malformation (spinal curvature). Treatment with the positive control chemical 3,3',5-triiodo-L-thyronine (T3) significantly decreased whole-body thyroxin (T4) concentrations, increased whole-body T3 concentrations, and upregulated mRNA expression involved in the HPT axis as a compensatory mechanism. These results suggested that the HPT axis in 144-hpf zebrafish larvae was responsive to chemical exposure and could be used to evaluate the effects of chemicals on the thyroid endocrine system. TDCPP exposure significantly decreased whole-body T4 concentrations and increased whole-body T3 concentrations, indicating thyroid endocrine disruption. The upregulation of genes related to thyroid hormone metabolism (dio1 and ugt1ab) might be responsible for decreased T4 concentrations. Treatment with TDCPP also significantly increased transcription of genes involved in thyroid hormone synthesis (tsh beta, slc5a5 and tg) and thyroid development (hhex, nkx2.1 and pax8) as a compensatory mechanism for decreased T4 concentrations. Taken together, these results suggest that TDCPP alters the transcription of genes involved in the HPT axis and changes whole-body concentrations of thyroid hormones in zebrafish embryos/larvae, thus causing an endocrine disruption of the thyroid system. (c) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordTdcpp Thyroid Endocrine Disruption Organophosphorus Flame Retardants Hpt Axis
Department[Wang, Qiangwei; Yang, Lihua; Guo, Yongyong; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Wang, Qiangwei] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Liang, Kang; Liu, Jingfu] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China; [Liu, Chunsheng] Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210046, Jiangsu, Peoples R China; [Liu, Chunsheng] Nanjing Univ, Sch Environm, Nanjing 210046, Jiangsu, Peoples R China
DOI10.1016/j.aquatox.2012.11.009
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [21207063, 20890113]; Natural Science Foundation of Jiangsu Province, China [BK2011032] ; National Natural Science Foundation of China [21207063, 20890113]; Natural Science Foundation of Jiangsu Province, China [BK2011032] ; National Natural Science Foundation of China [21207063, 20890113]; Natural Science Foundation of Jiangsu Province, China [BK2011032] ; National Natural Science Foundation of China [21207063, 20890113]; Natural Science Foundation of Jiangsu Province, China [BK2011032]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000315125600022
WOS KeywordUDP-GLUCURONOSYLTRANSFERASE INDUCERS ; ORGANOPHOSPHORUS FLAME RETARDANTS ; POLYBROMINATED DIPHENYL ETHERS ; ENDOCRINE DISRUPTION ; EXPRESSION ; RATS ; METABOLISM ; CELLS ; GLAND ; PLASTICIZERS
Funding OrganizationNational Natural Science Foundation of China [21207063, 20890113]; Natural Science Foundation of Jiangsu Province, China [BK2011032] ; National Natural Science Foundation of China [21207063, 20890113]; Natural Science Foundation of Jiangsu Province, China [BK2011032] ; National Natural Science Foundation of China [21207063, 20890113]; Natural Science Foundation of Jiangsu Province, China [BK2011032] ; National Natural Science Foundation of China [21207063, 20890113]; Natural Science Foundation of Jiangsu Province, China [BK2011032]
Citation statistics
Cited Times:116[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19329
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLiu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210046, Jiangsu, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
4.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210046, Jiangsu, Peoples R China
5.Nanjing Univ, Sch Environm, Nanjing 210046, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Wang, Qiangwei,Liang, Kang,Liu, Jingfu,et al. Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic-pituitary-thyroid axis[J]. AQUATIC TOXICOLOGY,2013,126(-):207-213.
APA Wang, Qiangwei.,Liang, Kang.,Liu, Jingfu.,Yang, Lihua.,Guo, Yongyong.,...&Liu, CS .(2013).Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic-pituitary-thyroid axis.AQUATIC TOXICOLOGY,126(-),207-213.
MLA Wang, Qiangwei,et al."Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic-pituitary-thyroid axis".AQUATIC TOXICOLOGY 126.-(2013):207-213.
Files in This Item: Download All
File Name/Size DocType Version Access License
Exposure of zebrafis(1196KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Qiangwei]'s Articles
[Liang, Kang]'s Articles
[Liu, Jingfu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Qiangwei]'s Articles
[Liang, Kang]'s Articles
[Liu, Jingfu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Qiangwei]'s Articles
[Liang, Kang]'s Articles
[Liu, Jingfu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Exposure of zebrafish embryos_larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic-pituitary-thyroid axis.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.