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题名: Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae
作者: Liu, Chunsheng1, 2; Wang, Qiangwei3; Liang, Kang4; Liu, Jingfu4; Zhou, Bingsheng3; Zhang, Xiaowei1, 2; Liu, Hongling1, 2; Giesy, John P.5, 6, 7, 8; Yu, Hongxia1, 2
通讯作者: Liu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210093, Jiangsu, Peoples R China.
关键词: TDCPP ; TPP ; mRNA expression ; Receptor ; Genomics
刊名: AQUATIC TOXICOLOGY
发表日期: 2013-03-15
DOI: 10.1016/j.aquatox.2012.12.010
卷: 128, 期:-, 页:147-157
收录类别: SCI
文章类型: Article
部门归属: [Liu, Chunsheng; Zhang, Xiaowei; Liu, Hongling; Yu, Hongxia] Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Jiangsu, Peoples R China; [Liu, Chunsheng; Zhang, Xiaowei; Liu, Hongling; Yu, Hongxia] Nanjing Univ, Sch Environm, Nanjing 210093, Jiangsu, Peoples R China; [Wang, Qiangwei; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Liang, Kang; Liu, Jingfu] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China; [Giesy, John P.] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada; [Giesy, John P.] Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada; [Giesy, John P.] Michigan State Univ, Ctr Integrat Toxicol, Dept Zool, E Lansing, MI 48824 USA; [Giesy, John P.] City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [21207063]; Natural Science Foundation of Jiangsu Province, China [BK2011032, BK2010384]; Canada Research Chair program, an at large Chair Professorship at the Department of Biology and Chemistry, City University of Hong Kong; State Key Laboratory in Marine Pollution, City University of Hong Kong; Einstein Professor Program of the Chinese Academy of Sciences
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) and triphenyl phosphate (TPP) are frequently detected in biota, including fish. However, knowledge of the toxicological and molecular effects of these currently used flame retardants is limited. In the present study, an in vivo screening approach was developed to evaluate effects of TDCPP and TPP on developmental endpoints and receptor-associated expression of mRNA in zebrafish embryos/larvae. Exposure to TDCPP or TPP resulted in significantly smaller rates of hatching and survival, in dose- and time-dependent manners. The median lethal concentration (LC50) was 7.0 mg/L for TDCPP and 29.6 mg/L for TPP at 120 hour post-fertilization (hpf). Real-time PCR revealed alterations in expression of mRNAs involved in aryl hydrocarbon receptors (AhRs)-, peroxisome proliferator-activated receptor alpha (PPAR alpha)-, estrogenic receptors (ERs)-, thyroid hormone receptor alpha (TR alpha)-, glucocorticoid receptor (GR)-, and mineralocorticoid receptor (MR)-centered gene networks. Exposure to positive control chemicals significantly altered abundances of mRNA in corresponding receptor-centered gene networks, a result that suggests that it is feasible to use zebrafish embryos/larvae to evaluate effects of chemicals on mRNA expression in these gene networks. Exposure to TDCPP altered transcriptional profiles in all six receptor-centered gene networks, thus exerting multiple toxic effects. TPP was easily metabolized and its potency to change expression of mRNA involved in receptor-centered gene networks was weaker than that of TDCPP. The PPAR alpha- and TR alpha-centered gene networks might be the primary pathways affected by TPP. Taken together, these results demonstrated that TDCPP and TPP could alter mRNA expression of genes involved in the six receptor-centered gene networks in zebrafish embryos/larvae, and TDCPP seemed to have higher potency in changing the mRNA expression of these genes. (c) 2012 Elsevier B.V. All rights reserved.
英文摘要: Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) and triphenyl phosphate (TPP) are frequently detected in biota, including fish. However, knowledge of the toxicological and molecular effects of these currently used flame retardants is limited. In the present study, an in vivo screening approach was developed to evaluate effects of TDCPP and TPP on developmental endpoints and receptor-associated expression of mRNA in zebrafish embryos/larvae. Exposure to TDCPP or TPP resulted in significantly smaller rates of hatching and survival, in dose- and time-dependent manners. The median lethal concentration (LC50) was 7.0 mg/L for TDCPP and 29.6 mg/L for TPP at 120 hour post-fertilization (hpf). Real-time PCR revealed alterations in expression of mRNAs involved in aryl hydrocarbon receptors (AhRs)-, peroxisome proliferator-activated receptor alpha (PPAR alpha)-, estrogenic receptors (ERs)-, thyroid hormone receptor alpha (TR alpha)-, glucocorticoid receptor (GR)-, and mineralocorticoid receptor (MR)-centered gene networks. Exposure to positive control chemicals significantly altered abundances of mRNA in corresponding receptor-centered gene networks, a result that suggests that it is feasible to use zebrafish embryos/larvae to evaluate effects of chemicals on mRNA expression in these gene networks. Exposure to TDCPP altered transcriptional profiles in all six receptor-centered gene networks, thus exerting multiple toxic effects. TPP was easily metabolized and its potency to change expression of mRNA involved in receptor-centered gene networks was weaker than that of TDCPP. The PPAR alpha- and TR alpha-centered gene networks might be the primary pathways affected by TPP. Taken together, these results demonstrated that TDCPP and TPP could alter mRNA expression of genes involved in the six receptor-centered gene networks in zebrafish embryos/larvae, and TDCPP seemed to have higher potency in changing the mRNA expression of these genes. (c) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: ORGANOPHOSPHORUS FLAME RETARDANTS ; ARYL-HYDROCARBON RECEPTOR ; SALMON SALMO-SALAR ; AH RECEPTOR ; PERFLUOROOCTANE SULFONATE ; ENDOCRINE DISRUPTION ; SURFACE WATERS ; TELEOST FISH ; PPAR-GAMMA ; CELLS
语种: 英语
WOS记录号: WOS:000316531900015
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19327
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Jiangsu, Peoples R China
2.Nanjing Univ, Sch Environm, Nanjing 210093, Jiangsu, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
5.Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada
6.Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada
7.Michigan State Univ, Ctr Integrat Toxicol, Dept Zool, E Lansing, MI 48824 USA
8.City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China
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