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Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae
Liu, Chunsheng1,2; Wang, Qiangwei3; Liang, Kang4; Liu, Jingfu4; Zhou, Bingsheng3; Zhang, Xiaowei1,2; Liu, Hongling1,2; Giesy, John P.5,6,7,8; Yu, Hongxia1,2; Liu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210093, Jiangsu, Peoples R China.
2013-03-15
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume128Issue:-Pages:147-157
AbstractTris(1,3-dichloro-2-propyl) phosphate (TDCPP) and triphenyl phosphate (TPP) are frequently detected in biota, including fish. However, knowledge of the toxicological and molecular effects of these currently used flame retardants is limited. In the present study, an in vivo screening approach was developed to evaluate effects of TDCPP and TPP on developmental endpoints and receptor-associated expression of mRNA in zebrafish embryos/larvae. Exposure to TDCPP or TPP resulted in significantly smaller rates of hatching and survival, in dose- and time-dependent manners. The median lethal concentration (LC50) was 7.0 mg/L for TDCPP and 29.6 mg/L for TPP at 120 hour post-fertilization (hpf). Real-time PCR revealed alterations in expression of mRNAs involved in aryl hydrocarbon receptors (AhRs)-, peroxisome proliferator-activated receptor alpha (PPAR alpha)-, estrogenic receptors (ERs)-, thyroid hormone receptor alpha (TR alpha)-, glucocorticoid receptor (GR)-, and mineralocorticoid receptor (MR)-centered gene networks. Exposure to positive control chemicals significantly altered abundances of mRNA in corresponding receptor-centered gene networks, a result that suggests that it is feasible to use zebrafish embryos/larvae to evaluate effects of chemicals on mRNA expression in these gene networks. Exposure to TDCPP altered transcriptional profiles in all six receptor-centered gene networks, thus exerting multiple toxic effects. TPP was easily metabolized and its potency to change expression of mRNA involved in receptor-centered gene networks was weaker than that of TDCPP. The PPAR alpha- and TR alpha-centered gene networks might be the primary pathways affected by TPP. Taken together, these results demonstrated that TDCPP and TPP could alter mRNA expression of genes involved in the six receptor-centered gene networks in zebrafish embryos/larvae, and TDCPP seemed to have higher potency in changing the mRNA expression of these genes. (c) 2012 Elsevier B.V. All rights reserved.; Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) and triphenyl phosphate (TPP) are frequently detected in biota, including fish. However, knowledge of the toxicological and molecular effects of these currently used flame retardants is limited. In the present study, an in vivo screening approach was developed to evaluate effects of TDCPP and TPP on developmental endpoints and receptor-associated expression of mRNA in zebrafish embryos/larvae. Exposure to TDCPP or TPP resulted in significantly smaller rates of hatching and survival, in dose- and time-dependent manners. The median lethal concentration (LC50) was 7.0 mg/L for TDCPP and 29.6 mg/L for TPP at 120 hour post-fertilization (hpf). Real-time PCR revealed alterations in expression of mRNAs involved in aryl hydrocarbon receptors (AhRs)-, peroxisome proliferator-activated receptor alpha (PPAR alpha)-, estrogenic receptors (ERs)-, thyroid hormone receptor alpha (TR alpha)-, glucocorticoid receptor (GR)-, and mineralocorticoid receptor (MR)-centered gene networks. Exposure to positive control chemicals significantly altered abundances of mRNA in corresponding receptor-centered gene networks, a result that suggests that it is feasible to use zebrafish embryos/larvae to evaluate effects of chemicals on mRNA expression in these gene networks. Exposure to TDCPP altered transcriptional profiles in all six receptor-centered gene networks, thus exerting multiple toxic effects. TPP was easily metabolized and its potency to change expression of mRNA involved in receptor-centered gene networks was weaker than that of TDCPP. The PPAR alpha- and TR alpha-centered gene networks might be the primary pathways affected by TPP. Taken together, these results demonstrated that TDCPP and TPP could alter mRNA expression of genes involved in the six receptor-centered gene networks in zebrafish embryos/larvae, and TDCPP seemed to have higher potency in changing the mRNA expression of these genes. (c) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordTdcpp Tpp Mrna Expression Receptor Genomics
Department[Liu, Chunsheng; Zhang, Xiaowei; Liu, Hongling; Yu, Hongxia] Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Jiangsu, Peoples R China; [Liu, Chunsheng; Zhang, Xiaowei; Liu, Hongling; Yu, Hongxia] Nanjing Univ, Sch Environm, Nanjing 210093, Jiangsu, Peoples R China; [Wang, Qiangwei; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Liang, Kang; Liu, Jingfu] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China; [Giesy, John P.] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada; [Giesy, John P.] Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada; [Giesy, John P.] Michigan State Univ, Ctr Integrat Toxicol, Dept Zool, E Lansing, MI 48824 USA; [Giesy, John P.] City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China
DOI10.1016/j.aquatox.2012.12.010
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [21207063]; Natural Science Foundation of Jiangsu Province, China [BK2011032, BK2010384]; Canada Research Chair program, an at large Chair Professorship at the Department of Biology and Chemistry, City University of Hong Kong; State Key Laboratory in Marine Pollution, City University of Hong Kong; Einstein Professor Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China [21207063]; Natural Science Foundation of Jiangsu Province, China [BK2011032, BK2010384]; Canada Research Chair program, an at large Chair Professorship at the Department of Biology and Chemistry, City University of Hong Kong; State Key Laboratory in Marine Pollution, City University of Hong Kong; Einstein Professor Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China [21207063]; Natural Science Foundation of Jiangsu Province, China [BK2011032, BK2010384]; Canada Research Chair program, an at large Chair Professorship at the Department of Biology and Chemistry, City University of Hong Kong; State Key Laboratory in Marine Pollution, City University of Hong Kong; Einstein Professor Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China [21207063]; Natural Science Foundation of Jiangsu Province, China [BK2011032, BK2010384]; Canada Research Chair program, an at large Chair Professorship at the Department of Biology and Chemistry, City University of Hong Kong; State Key Laboratory in Marine Pollution, City University of Hong Kong; Einstein Professor Program of the Chinese Academy of Sciences
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000316531900015
WOS KeywordORGANOPHOSPHORUS FLAME RETARDANTS ; ARYL-HYDROCARBON RECEPTOR ; SALMON SALMO-SALAR ; AH RECEPTOR ; PERFLUOROOCTANE SULFONATE ; ENDOCRINE DISRUPTION ; SURFACE WATERS ; TELEOST FISH ; PPAR-GAMMA ; CELLS
Funding OrganizationNational Natural Science Foundation of China [21207063]; Natural Science Foundation of Jiangsu Province, China [BK2011032, BK2010384]; Canada Research Chair program, an at large Chair Professorship at the Department of Biology and Chemistry, City University of Hong Kong; State Key Laboratory in Marine Pollution, City University of Hong Kong; Einstein Professor Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China [21207063]; Natural Science Foundation of Jiangsu Province, China [BK2011032, BK2010384]; Canada Research Chair program, an at large Chair Professorship at the Department of Biology and Chemistry, City University of Hong Kong; State Key Laboratory in Marine Pollution, City University of Hong Kong; Einstein Professor Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China [21207063]; Natural Science Foundation of Jiangsu Province, China [BK2011032, BK2010384]; Canada Research Chair program, an at large Chair Professorship at the Department of Biology and Chemistry, City University of Hong Kong; State Key Laboratory in Marine Pollution, City University of Hong Kong; Einstein Professor Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China [21207063]; Natural Science Foundation of Jiangsu Province, China [BK2011032, BK2010384]; Canada Research Chair program, an at large Chair Professorship at the Department of Biology and Chemistry, City University of Hong Kong; State Key Laboratory in Marine Pollution, City University of Hong Kong; Einstein Professor Program of the Chinese Academy of Sciences
Citation statistics
Cited Times:84[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19327
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLiu, CS (reprint author), Nanjing Univ, Sch Environm, Nanjing 210093, Jiangsu, Peoples R China.
Affiliation1.Nanjing Univ, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Jiangsu, Peoples R China
2.Nanjing Univ, Sch Environm, Nanjing 210093, Jiangsu, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
5.Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada
6.Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada
7.Michigan State Univ, Ctr Integrat Toxicol, Dept Zool, E Lansing, MI 48824 USA
8.City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Liu, Chunsheng,Wang, Qiangwei,Liang, Kang,et al. Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae[J]. AQUATIC TOXICOLOGY,2013,128(-):147-157.
APA Liu, Chunsheng.,Wang, Qiangwei.,Liang, Kang.,Liu, Jingfu.,Zhou, Bingsheng.,...&Liu, CS .(2013).Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae.AQUATIC TOXICOLOGY,128(-),147-157.
MLA Liu, Chunsheng,et al."Effects of tris(1,3-dichloro-2-propyl) phosphate and triphenyl phosphate on receptor-associated mRNA expression in zebrafish embryos/larvae".AQUATIC TOXICOLOGY 128.-(2013):147-157.
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