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An improved method for determining phytoplankton chlorophyll a concentration without filtration
Qin, Hongjie1,2; Li, Shuangshuang1,2; Li, Dunhai1; Li, DH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-05-01
Source PublicationHYDROBIOLOGIA
ISSN0018-8158
Volume707Issue:1Pages:81-95
AbstractThe common and routine procedure for the quantification of chlorophyll a (chl a) in aquatic studies has a series of steps. Here, we sought to find optimal conditions for phytoplankton cell harvesting, chl a extraction, and chl a measurement and calculation, to find an effective, cost-saving, safe, and environment-friendly procedure for determining phytoplankton chl a concentration. We replaced the traditional GF/C filters with inorganic polymer flocculants (IPFs) and clay for phytoplankton harvesting, and then various solvents (acetone, ethanol, DMF, and DMSO), IPFs (PAC, PFS, and PAFS) and clay were tested for their suitability for chl a extraction, with or without homogenization at different temperatures for different extraction durations. About 0.3-1.0 g l(-1) of PAC or PFSA combined with 1.0-2.5 g l(-1) clay were found to provide optimal conditions in terms of yield and cost for phytoplankton cell harvesting from water samples. Based on our results, we recommend flocculation and centrifugation instead of glass-fiber membrane filtration for harvesting phytoplankton cells from environmental water samples, 95% ethanol for chl a extraction without homogenization and heating, and spectrophotometry to determine chl a concentration.; The common and routine procedure for the quantification of chlorophyll a (chl a) in aquatic studies has a series of steps. Here, we sought to find optimal conditions for phytoplankton cell harvesting, chl a extraction, and chl a measurement and calculation, to find an effective, cost-saving, safe, and environment-friendly procedure for determining phytoplankton chl a concentration. We replaced the traditional GF/C filters with inorganic polymer flocculants (IPFs) and clay for phytoplankton harvesting, and then various solvents (acetone, ethanol, DMF, and DMSO), IPFs (PAC, PFS, and PAFS) and clay were tested for their suitability for chl a extraction, with or without homogenization at different temperatures for different extraction durations. About 0.3-1.0 g l(-1) of PAC or PFSA combined with 1.0-2.5 g l(-1) clay were found to provide optimal conditions in terms of yield and cost for phytoplankton cell harvesting from water samples. Based on our results, we recommend flocculation and centrifugation instead of glass-fiber membrane filtration for harvesting phytoplankton cells from environmental water samples, 95% ethanol for chl a extraction without homogenization and heating, and spectrophotometry to determine chl a concentration.
SubtypeArticle
KeywordChlorophyll a Clay Cyanobacteria Extraction Solvent Flocculation Spectrophotometry
Department[Qin, Hongjie ; Li, Shuangshuang ; Li, Dunhai] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Qin, Hongjie ; Li, Shuangshuang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
DOI10.1007/s10750-012-1412-6
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002] ; National Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002] ; National Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002] ; National Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000316332300007
WOS KeywordSPECTROPHOTOMETRIC DETERMINATION ; ETHANOL SOLVENTS ; EXTRACTION ; EQUATIONS ; MICROALGAE ; PIGMENTS ; METHANOL ; ACETONE ; BLOOMS ; WASTE
Funding OrganizationNational Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002] ; National Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002] ; National Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002] ; National Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19325
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLi, DH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Qin, Hongjie,Li, Shuangshuang,Li, Dunhai,et al. An improved method for determining phytoplankton chlorophyll a concentration without filtration[J]. HYDROBIOLOGIA,2013,707(1):81-95.
APA Qin, Hongjie,Li, Shuangshuang,Li, Dunhai,&Li, DH .(2013).An improved method for determining phytoplankton chlorophyll a concentration without filtration.HYDROBIOLOGIA,707(1),81-95.
MLA Qin, Hongjie,et al."An improved method for determining phytoplankton chlorophyll a concentration without filtration".HYDROBIOLOGIA 707.1(2013):81-95.
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