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题名: An improved method for determining phytoplankton chlorophyll a concentration without filtration
作者: Qin, Hongjie1, 2; Li, Shuangshuang1, 2; Li, Dunhai1
通讯作者: Li, DH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Chlorophyll a ; Clay ; Cyanobacteria ; Extraction solvent ; Flocculation ; Spectrophotometry
刊名: HYDROBIOLOGIA
发表日期: 2013-05-01
DOI: 10.1007/s10750-012-1412-6
卷: 707, 期:1, 页:81-95
收录类别: SCI
文章类型: Article
部门归属: [Qin, Hongjie ; Li, Shuangshuang ; Li, Dunhai] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Qin, Hongjie ; Li, Shuangshuang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002]
类目[WOS]: Marine & Freshwater Biology
研究领域[WOS]: Marine & Freshwater Biology
摘要: The common and routine procedure for the quantification of chlorophyll a (chl a) in aquatic studies has a series of steps. Here, we sought to find optimal conditions for phytoplankton cell harvesting, chl a extraction, and chl a measurement and calculation, to find an effective, cost-saving, safe, and environment-friendly procedure for determining phytoplankton chl a concentration. We replaced the traditional GF/C filters with inorganic polymer flocculants (IPFs) and clay for phytoplankton harvesting, and then various solvents (acetone, ethanol, DMF, and DMSO), IPFs (PAC, PFS, and PAFS) and clay were tested for their suitability for chl a extraction, with or without homogenization at different temperatures for different extraction durations. About 0.3-1.0 g l(-1) of PAC or PFSA combined with 1.0-2.5 g l(-1) clay were found to provide optimal conditions in terms of yield and cost for phytoplankton cell harvesting from water samples. Based on our results, we recommend flocculation and centrifugation instead of glass-fiber membrane filtration for harvesting phytoplankton cells from environmental water samples, 95% ethanol for chl a extraction without homogenization and heating, and spectrophotometry to determine chl a concentration.
英文摘要: The common and routine procedure for the quantification of chlorophyll a (chl a) in aquatic studies has a series of steps. Here, we sought to find optimal conditions for phytoplankton cell harvesting, chl a extraction, and chl a measurement and calculation, to find an effective, cost-saving, safe, and environment-friendly procedure for determining phytoplankton chl a concentration. We replaced the traditional GF/C filters with inorganic polymer flocculants (IPFs) and clay for phytoplankton harvesting, and then various solvents (acetone, ethanol, DMF, and DMSO), IPFs (PAC, PFS, and PAFS) and clay were tested for their suitability for chl a extraction, with or without homogenization at different temperatures for different extraction durations. About 0.3-1.0 g l(-1) of PAC or PFSA combined with 1.0-2.5 g l(-1) clay were found to provide optimal conditions in terms of yield and cost for phytoplankton cell harvesting from water samples. Based on our results, we recommend flocculation and centrifugation instead of glass-fiber membrane filtration for harvesting phytoplankton cells from environmental water samples, 95% ethanol for chl a extraction without homogenization and heating, and spectrophotometry to determine chl a concentration.
关键词[WOS]: SPECTROPHOTOMETRIC DETERMINATION ; ETHANOL SOLVENTS ; EXTRACTION ; EQUATIONS ; MICROALGAE ; PIGMENTS ; METHANOL ; ACETONE ; BLOOMS ; WASTE
语种: 英语
WOS记录号: WOS:000316332300007
ISSN号: 0018-8158
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19325
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Qin, HJ; Li, SS; Li, DH.An improved method for determining phytoplankton chlorophyll a concentration without filtration,HYDROBIOLOGIA,2013,707(1):81-95
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