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题名: Acute exposure to DE-71 causes alterations in visual behavior in zebrafish larvae
作者: Chen, Lianguo1; Huang, Yubin2; Huang, Changjiang3; Hu, Bing2; Hu, Chenyan1; Zhou, Bingsheng1
通讯作者: Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
关键词: Polybrominated diphenyl ethers ; Retinal structure ; Visual behavior ; Zebrafish larvae
刊名: ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
发表日期: 2013-06-01
DOI: 10.1002/etc.2168
卷: 32, 期:6, 页:1370-1375
收录类别: SCI
文章类型: Article
部门归属: [Chen, Lianguo ; Hu, Chenyan ; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; [Huang, Yubin ; Hu, Bing] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China ; [Huang, Changjiang] Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [20890113, 21237005]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
类目[WOS]: Environmental Sciences ; Toxicology
研究领域[WOS]: Environmental Sciences & Ecology ; Toxicology
摘要: Polybrominated diphenyl ethers (PBDEs) cause neurobehavioral toxicity, but their effects on visual behavior remain unknown. In the present study, the impact of PBDEs on visual behavior was examined using optokinetic responses and phototaxis in zebrafish larvae. Zebrafish embryos were exposed to pentabrominated diphenyl ethers mixture (DE-71) at concentrations of 0, 0.32, 3.58, and 31.0 mu g/L until 15 d postfertilization. The authors then assessed photoreceptor opsin expression, retinal histology, and visual behavior of the larvae. The results showed that the transcriptions of the opsin genes, zfrho and zfgr1, were significantly upregulated. Western blotting further demonstrated a significant increase in rhodopsin protein expression after exposure of the larvae to DE-71. Histological examination revealed the following morphological alterations in the retina: increased area of inner nuclear layer, decreased area of inner plexiform layer, and decreased density of ganglion cells. Tests of optokinetic and phototactic behavior showed hyperactive responses on exposure to DE-71, including increased saccadic eye movements and phototactic response. The present study is the first to demonstrate that the acute exposure of zebrafish larvae to DE-71 causes biochemical and structural changes in the eye that lead to behavioral alterations. Analysis of these visual behavioral paradigms may be useful in predicting the adverse effects of toxicants on visual function in fish. Environ Toxicol Chem 2013;32:13701375. (c) 2013 SETAC
英文摘要: Polybrominated diphenyl ethers (PBDEs) cause neurobehavioral toxicity, but their effects on visual behavior remain unknown. In the present study, the impact of PBDEs on visual behavior was examined using optokinetic responses and phototaxis in zebrafish larvae. Zebrafish embryos were exposed to pentabrominated diphenyl ethers mixture (DE-71) at concentrations of 0, 0.32, 3.58, and 31.0 mu g/L until 15 d postfertilization. The authors then assessed photoreceptor opsin expression, retinal histology, and visual behavior of the larvae. The results showed that the transcriptions of the opsin genes, zfrho and zfgr1, were significantly upregulated. Western blotting further demonstrated a significant increase in rhodopsin protein expression after exposure of the larvae to DE-71. Histological examination revealed the following morphological alterations in the retina: increased area of inner nuclear layer, decreased area of inner plexiform layer, and decreased density of ganglion cells. Tests of optokinetic and phototactic behavior showed hyperactive responses on exposure to DE-71, including increased saccadic eye movements and phototactic response. The present study is the first to demonstrate that the acute exposure of zebrafish larvae to DE-71 causes biochemical and structural changes in the eye that lead to behavioral alterations. Analysis of these visual behavioral paradigms may be useful in predicting the adverse effects of toxicants on visual function in fish. Environ Toxicol Chem 2013;32:13701375. (c) 2013 SETAC
关键词[WOS]: POLYBROMINATED DIPHENYL ETHERS ; DANIO-RERIO ; VITAMIN-A ; DEVELOPMENTAL NEUROTOXICITY ; PHOTORECEPTOR DEVELOPMENT ; THYROID-HORMONE ; PBDES ; RETINA ; MODEL ; REGENERATION
语种: 英语
WOS记录号: WOS:000318233100025
ISSN号: 0730-7268
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19322
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
3.Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou, Peoples R China

Recommended Citation:
Chen, LG; Huang, YB; Huang, CJ; Hu, B; Hu, CY; Zhou, BS.Acute exposure to DE-71 causes alterations in visual behavior in zebrafish larvae,ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2013,32(6):1370-1375
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