IHB OpenIR  > 水环境工程研究中心  > 期刊论文
Acute exposure to DE-71 causes alterations in visual behavior in zebrafish larvae
Chen, Lianguo1; Huang, Yubin2; Huang, Changjiang3; Hu, Bing2; Hu, Chenyan1; Zhou, Bingsheng1; Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
2013-06-01
Source PublicationENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
ISSN0730-7268
Volume32Issue:6Pages:1370-1375
AbstractPolybrominated diphenyl ethers (PBDEs) cause neurobehavioral toxicity, but their effects on visual behavior remain unknown. In the present study, the impact of PBDEs on visual behavior was examined using optokinetic responses and phototaxis in zebrafish larvae. Zebrafish embryos were exposed to pentabrominated diphenyl ethers mixture (DE-71) at concentrations of 0, 0.32, 3.58, and 31.0 mu g/L until 15 d postfertilization. The authors then assessed photoreceptor opsin expression, retinal histology, and visual behavior of the larvae. The results showed that the transcriptions of the opsin genes, zfrho and zfgr1, were significantly upregulated. Western blotting further demonstrated a significant increase in rhodopsin protein expression after exposure of the larvae to DE-71. Histological examination revealed the following morphological alterations in the retina: increased area of inner nuclear layer, decreased area of inner plexiform layer, and decreased density of ganglion cells. Tests of optokinetic and phototactic behavior showed hyperactive responses on exposure to DE-71, including increased saccadic eye movements and phototactic response. The present study is the first to demonstrate that the acute exposure of zebrafish larvae to DE-71 causes biochemical and structural changes in the eye that lead to behavioral alterations. Analysis of these visual behavioral paradigms may be useful in predicting the adverse effects of toxicants on visual function in fish. Environ Toxicol Chem 2013;32:13701375. (c) 2013 SETAC; Polybrominated diphenyl ethers (PBDEs) cause neurobehavioral toxicity, but their effects on visual behavior remain unknown. In the present study, the impact of PBDEs on visual behavior was examined using optokinetic responses and phototaxis in zebrafish larvae. Zebrafish embryos were exposed to pentabrominated diphenyl ethers mixture (DE-71) at concentrations of 0, 0.32, 3.58, and 31.0 mu g/L until 15 d postfertilization. The authors then assessed photoreceptor opsin expression, retinal histology, and visual behavior of the larvae. The results showed that the transcriptions of the opsin genes, zfrho and zfgr1, were significantly upregulated. Western blotting further demonstrated a significant increase in rhodopsin protein expression after exposure of the larvae to DE-71. Histological examination revealed the following morphological alterations in the retina: increased area of inner nuclear layer, decreased area of inner plexiform layer, and decreased density of ganglion cells. Tests of optokinetic and phototactic behavior showed hyperactive responses on exposure to DE-71, including increased saccadic eye movements and phototactic response. The present study is the first to demonstrate that the acute exposure of zebrafish larvae to DE-71 causes biochemical and structural changes in the eye that lead to behavioral alterations. Analysis of these visual behavioral paradigms may be useful in predicting the adverse effects of toxicants on visual function in fish. Environ Toxicol Chem 2013;32:13701375. (c) 2013 SETAC
SubtypeArticle
KeywordPolybrominated Diphenyl Ethers Retinal Structure Visual Behavior Zebrafish Larvae
Department[Chen, Lianguo ; Hu, Chenyan ; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; [Huang, Yubin ; Hu, Bing] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China ; [Huang, Changjiang] Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou, Peoples R China
DOI10.1002/etc.2168
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [20890113, 21237005]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113, 21237005]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113, 21237005]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113, 21237005]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000318233100025
WOS KeywordPOLYBROMINATED DIPHENYL ETHERS ; DANIO-RERIO ; VITAMIN-A ; DEVELOPMENTAL NEUROTOXICITY ; PHOTORECEPTOR DEVELOPMENT ; THYROID-HORMONE ; PBDES ; RETINA ; MODEL ; REGENERATION
Funding OrganizationNational Natural Science Foundation of China [20890113, 21237005]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113, 21237005]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113, 21237005]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113, 21237005]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19322
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
3.Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou, Peoples R China
Recommended Citation
GB/T 7714
Chen, Lianguo,Huang, Yubin,Huang, Changjiang,et al. Acute exposure to DE-71 causes alterations in visual behavior in zebrafish larvae[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2013,32(6):1370-1375.
APA Chen, Lianguo.,Huang, Yubin.,Huang, Changjiang.,Hu, Bing.,Hu, Chenyan.,...&Zhou, BS .(2013).Acute exposure to DE-71 causes alterations in visual behavior in zebrafish larvae.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,32(6),1370-1375.
MLA Chen, Lianguo,et al."Acute exposure to DE-71 causes alterations in visual behavior in zebrafish larvae".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 32.6(2013):1370-1375.
Files in This Item:
File Name/Size DocType Version Access License
Acute exposure to DE(489KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Lianguo]'s Articles
[Huang, Yubin]'s Articles
[Huang, Changjiang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Lianguo]'s Articles
[Huang, Yubin]'s Articles
[Huang, Changjiang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Lianguo]'s Articles
[Huang, Yubin]'s Articles
[Huang, Changjiang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Acute exposure to DE-71 causes alterations in visual behavior in zebrafish larvae.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.