IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis
Ye, Huan1,4; Chen, Xihua1; Wei, Qiwei1,3; Zhou, Li2; Liu, Tao4; Gui, Jianfang2; Li, Chuangju1,3; Cao, Hong2; Li, CJ (reprint author), Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minisny Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China.
2012-12-15
Source PublicationGENE
ISSN0378-1119
Volume511Issue:2Pages:285-292
AbstractThe nanos gene family was essential for germ line development in diverse organisms. In the present study, the full-length cDNA of a nanos1 homologue in A. sinensis, Asnanos1, was isolated and characterized. The cDNA sequence of Asnanos1 was 1489 base pairs (bp) in length and encoded a peptide of 228 amino acid residues. Multiple sequence alignment showed that the zinc-finger motifs of Nanos1 were highly conserved in vertebrates. By RT-PCR analysis, Asnanos1 mRNAs were ubiquitously detected in all tissues examined except for the fat, including liver, spleen, heart, ovary, kidney, muscle, intestines, pituitary, hypothalamus, telencephalon, midbrain, cerebellum, and medulla oblongata. Moreover, a specific polyclonal antibody was prepared from the in vitro expressed partial AsNanos1 protein. Western blot analysis revealed that the tissue expression pattern of AsNanos1 was not completely coincided with that of its mRNAs, which was not found in fat, muscle and intestines. Additionally, by immunofluoresence localization, it was observed that AsNanos1 protein was in the cytoplasm of primary oocytes and spermatocytes. The presented results indicated that the expression pattern of Asnanos1 was differential conservation and divergence among diverse species. (C) 2012 Elsevier B.V. All rights reserved.; The nanos gene family was essential for germ line development in diverse organisms. In the present study, the full-length cDNA of a nanos1 homologue in A. sinensis, Asnanos1, was isolated and characterized. The cDNA sequence of Asnanos1 was 1489 base pairs (bp) in length and encoded a peptide of 228 amino acid residues. Multiple sequence alignment showed that the zinc-finger motifs of Nanos1 were highly conserved in vertebrates. By RT-PCR analysis, Asnanos1 mRNAs were ubiquitously detected in all tissues examined except for the fat, including liver, spleen, heart, ovary, kidney, muscle, intestines, pituitary, hypothalamus, telencephalon, midbrain, cerebellum, and medulla oblongata. Moreover, a specific polyclonal antibody was prepared from the in vitro expressed partial AsNanos1 protein. Western blot analysis revealed that the tissue expression pattern of AsNanos1 was not completely coincided with that of its mRNAs, which was not found in fat, muscle and intestines. Additionally, by immunofluoresence localization, it was observed that AsNanos1 protein was in the cytoplasm of primary oocytes and spermatocytes. The presented results indicated that the expression pattern of Asnanos1 was differential conservation and divergence among diverse species. (C) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordNanos1 Chinese Sturgeon Expression Pattern
Department[Ye, Huan ; Chen, Xihua ; Wei, Qiwei ; Li, Chuangju] Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minisny Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China ; [Zhou, Li ; Gui, Jianfang ; Cao, Hong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Wei, Qiwei ; Li, Chuangju] Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China ; [Ye, Huan ; Liu, Tao] Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
DOI10.1016/j.gene.2012.09.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences [2011JBFA22]; National Natural Science Foundation of China [31001104]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences [2011JBFA22]; National Natural Science Foundation of China [31001104]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences [2011JBFA22]; National Natural Science Foundation of China [31001104]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences [2011JBFA22]; National Natural Science Foundation of China [31001104]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000313024700021
WOS KeywordGERM-CELL DEVELOPMENT ; CAENORHABDITIS-ELEGANS ; DENDRITE MORPHOGENESIS ; PLASM COMPONENT ; STEM-CELLS ; GENES ; RNA ; CONSERVATION ; POSTERIOR ; PROTEINS
Funding OrganizationNational Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences [2011JBFA22]; National Natural Science Foundation of China [31001104]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences [2011JBFA22]; National Natural Science Foundation of China [31001104]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences [2011JBFA22]; National Natural Science Foundation of China [31001104]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences [2011JBFA22]; National Natural Science Foundation of China [31001104]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]
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Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19319
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLi, CJ (reprint author), Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minisny Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China.
Affiliation1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minisny Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
Recommended Citation
GB/T 7714
Ye, Huan,Chen, Xihua,Wei, Qiwei,et al. Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis[J]. GENE,2012,511(2):285-292.
APA Ye, Huan.,Chen, Xihua.,Wei, Qiwei.,Zhou, Li.,Liu, Tao.,...&Li, CJ .(2012).Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis.GENE,511(2),285-292.
MLA Ye, Huan,et al."Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis".GENE 511.2(2012):285-292.
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