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题名: Transcriptome analysis of head kidney in grass carp and discovery of immune-related genes
作者: Chen, Jin1, 2; Li, Cai1, 2; Huang, Rong1; Du, Fukuan1, 2; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
通讯作者: Wang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: Grass carp ; Head kidney ; cDNA ; EST ; Immune-related gene
刊名: BMC VETERINARY RESEARCH
发表日期: 2012-07-09
DOI: 10.1186/1746-6148-8-108
卷: 8
收录类别: SCI
文章类型: Article
部门归属: [Chen, Jin ; Li, Cai ; Huang, Rong ; Du, Fukuan ; Liao, Lanjie ; Zhu, Zuoyan ; Wang, Yaping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Chen, Jin ; Li, Cai ; Du, Fukuan] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Innovation Project of the Chinese Academy of Sciences [KSCX2-EW-N-004-3]; National Key Basic Research Program [2009CB118701]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ18]
类目[WOS]: Veterinary Sciences
研究领域[WOS]: Veterinary Sciences
摘要: Background: Grass carp (Ctenopharyngodon idella) is one of the most economically important freshwater fish, but its production is often affected by diseases that cause serious economic losses. To date, no good breeding varieties have been obtained using the oriented cultivation technique. The ability to identify disease resistance genes in grass carp is important to cultivate disease-resistant varieties of grass carp. Results: In this study, we constructed a non-normalized cDNA library of head kidney in grass carp, and, after clustering and assembly, we obtained 3,027 high-quality unigenes. Solexa sequencing was used to generate sequence tags from the transcriptomes of the head kidney in grass carp before and after grass carp reovirus (GCRV) infection. After processing, we obtained 22,144 tags that were differentially expressed by more than 2-fold between the uninfected and infected groups. 679 of the differentially expressed tags (3.1%) mapped to 483 of the unigenes (16.0%). The up-regulated and down-regulated unigenes were annotated using gene ontology terms; 16 were annotated as immune-related and 42 were of unknown function having no matches to any of the sequences in the databases that were used in the similarity searches. Semi-quantitative RT-PCR revealed four unknown unigenes that showed significant responses to the viral infection. Based on domain structure predictions, one of these sequences was found to encode a protein that contained two transmembrane domains and, therefore, may be a transmembrane protein. Here, we proposed that this novel unigene may encode a virus receptor or a protein that mediates the immune signalling pathway at the cell surface. Conclusion: This study enriches the molecular basis data of grass carp and further confirms that, based on fish tissue-specific EST databases, transcriptome analysis is an effective route to discover novel functional genes.
英文摘要: Background: Grass carp (Ctenopharyngodon idella) is one of the most economically important freshwater fish, but its production is often affected by diseases that cause serious economic losses. To date, no good breeding varieties have been obtained using the oriented cultivation technique. The ability to identify disease resistance genes in grass carp is important to cultivate disease-resistant varieties of grass carp.
关键词[WOS]: EXPRESSED SEQUENCE TAGS ; HALIBUT HIPPOGLOSSUS-HIPPOGLOSSUS ; MESSENGER-RNA TRANSCRIPTS ; BASS DICENTRARCHUS-LABRAX ; GENOME ANNOTATION ; CHANNEL CATFISH ; EST ANALYSIS ; PROFILES ; LIVER ; MICROARRAY
语种: 英语
WOS记录号: WOS:000312219400001
ISSN号: 1746-6148
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19317
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Chen, J; Li, C; Huang, R; Du, FK; Liao, LJ; Zhu, ZY; Wang, YP.Transcriptome analysis of head kidney in grass carp and discovery of immune-related genes,BMC VETERINARY RESEARCH,2012,8():
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