IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Sequencing and expression analysis of CD3 gamma/delta and CD3E > chains in mandarin fish, Siniperca chuatsi
Guo Zheng1,2; Nie Pin1; Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
Volume31Issue:1Pages:106-117
AbstractThe genomic and cDNA sequences of the CD3 gamma/delta and CD3E > homologues in the mandarin fish, Siniperca chuats i, were determined. As in other vertebrate CD3 molecules, the deduced amino acid sequences of mandarin fish CD3 gamma/delta and CD3E > contained conserved residues and motifs, such as cysteine residues and CXXC and immunoreceptor tyrosine-based activation motifs. However, mandarin fish CD3 gamma/delta and CD3E > showed some differences to their mammalian counterparts, specifically the absence of a negatively charged residue in the transmembrane region of CD3 gamma/delta. Additionally, while an N -glycosylation site was present in CD3E >, the site was not observed in CD3 gamma/delta. The CD3 gamma/delta and CD3E > subunit sequences contain six and five exons, respectively, consistent with homologues from Atlantic salmon, Salmo salar. Phylogenetic analysis also revealed that CD3 gamma/delta and CD3E > in mandarin fish are closely related to their counterparts in Acanthopterygian fish. Real-time PCR showed CD3 gamma/delta and CD3E > were expressed mainly in the thymus and spleen in normal healthy fish and, to a lesser extent, in mucosal-associated lymphoid tissues, such as the intestine and gills. When lymphocytes isolated from head kidney were treated with the mitogens phytohemagglutinin, concanavalin, and polyriboinosinic polyribocytidylic acid, mRNA expression levels of CD3 gamma/delta and CD3E > were significantly elevated within 12 h of treatment. This indicated the presence of T lymphocytes in the head kidney of teleost fish, and also the recognition of mitogens by the lymphocytes. Mandarin fish infected with the bacterial pathogen Flavobacterium columnare also showed an increase in the expression of CD3 gamma/delta and CD3E > mRNA, indicating that CD3 gamma/delta and CD3E > lymphocytes are involved in the immune response of this species.; The genomic and cDNA sequences of the CD3 gamma/delta and CD3E > homologues in the mandarin fish, Siniperca chuats i, were determined. As in other vertebrate CD3 molecules, the deduced amino acid sequences of mandarin fish CD3 gamma/delta and CD3E > contained conserved residues and motifs, such as cysteine residues and CXXC and immunoreceptor tyrosine-based activation motifs. However, mandarin fish CD3 gamma/delta and CD3E > showed some differences to their mammalian counterparts, specifically the absence of a negatively charged residue in the transmembrane region of CD3 gamma/delta. Additionally, while an N -glycosylation site was present in CD3E >, the site was not observed in CD3 gamma/delta. The CD3 gamma/delta and CD3E > subunit sequences contain six and five exons, respectively, consistent with homologues from Atlantic salmon, Salmo salar. Phylogenetic analysis also revealed that CD3 gamma/delta and CD3E > in mandarin fish are closely related to their counterparts in Acanthopterygian fish. Real-time PCR showed CD3 gamma/delta and CD3E > were expressed mainly in the thymus and spleen in normal healthy fish and, to a lesser extent, in mucosal-associated lymphoid tissues, such as the intestine and gills. When lymphocytes isolated from head kidney were treated with the mitogens phytohemagglutinin, concanavalin, and polyriboinosinic polyribocytidylic acid, mRNA expression levels of CD3 gamma/delta and CD3E > were significantly elevated within 12 h of treatment. This indicated the presence of T lymphocytes in the head kidney of teleost fish, and also the recognition of mitogens by the lymphocytes. Mandarin fish infected with the bacterial pathogen Flavobacterium columnare also showed an increase in the expression of CD3 gamma/delta and CD3E > mRNA, indicating that CD3 gamma/delta and CD3E > lymphocytes are involved in the immune response of this species.
SubtypeArticle
KeywordCd3 Cd3 Gamma/delta Cd3 Epsilon Mandarin Fish Mitogen Flavobacterium Columnare
Department[Guo Zheng; Nie Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Guo Zheng] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
DOI10.1007/s00343-013-2018-1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationNational Natural Science Foundation of China [U0631010]; Government of Guangdong Province; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province; National Basic Research Program of China (973 Program) [2009CB118703]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000313654000012
WOS KeywordT-CELL-RECEPTOR ; BASS DICENTRARCHUS-LABRAX ; FLAVOBACTERIUM-COLUMNARE ; GENE-EXPRESSION ; TELEOST FISH ; CD3 GAMMA ; MOLECULAR CHARACTERIZATION ; PARALICHTHYS-OLIVACEUS ; JAPANESE FLOUNDER ; ATLANTIC SALMON
Funding OrganizationNational Natural Science Foundation of China [U0631010]; Government of Guangdong Province; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province; National Basic Research Program of China (973 Program) [2009CB118703]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19313
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Guo Zheng,Nie Pin,Nie, P . Sequencing and expression analysis of CD3 gamma/delta and CD3E > chains in mandarin fish, Siniperca chuatsi[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(1):106-117.
APA Guo Zheng,Nie Pin,&Nie, P .(2013).Sequencing and expression analysis of CD3 gamma/delta and CD3E > chains in mandarin fish, Siniperca chuatsi.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(1),106-117.
MLA Guo Zheng,et al."Sequencing and expression analysis of CD3 gamma/delta and CD3E > chains in mandarin fish, Siniperca chuatsi".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.1(2013):106-117.
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