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题名: Characterization and expression of Cd8 molecules in mandarin fish Siniperca chuatsi
作者: Guo, Z.1, 2; Wang, G. L.1, 2; Fu, J. P.1; Nie, P.1
通讯作者: Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: cd8a ; cd8 ss ; immunohistochemistry ; stimulation
刊名: JOURNAL OF FISH BIOLOGY
发表日期: 2013
DOI: 10.1111/j.1095-8649.2012.03475.x
卷: 82, 期:1, 页:189-205
收录类别: SCI
文章类型: Article
部门归属: [Guo, Z. ; Wang, G. L. ; Fu, J. P. ; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Guo, Z. ; Wang, G. L.] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [U0631010]; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703]
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
摘要: The full-length complementary DNA (cDNA) sequences encoding cd8a and cd8 beta molecules were sequenced and characterized from mandarin fish Siniperca chuatsi. Conserved motifs and residues were found to be present in derived peptides of the Cd8 molecules. For example, WXR motif, DXGXYXC motif, and four cysteine residues were present in the extracellular region of the Cd8 protein. Threonine, serine and proline residues involved in multiple O-linked glycosylation events were located in the membrane proximal hinge region. The common CPH motif in the cytoplasmic tail was detected similar to other teleost Cd8 molecules. Different from those in mammals, S. chuatsi Cd8 sequences have many extra cysteine residues (C149 in Cd8a sequence and C46, C51 and C158 in Cd8 beta sequence), which also exist in other teleost Cd8 molecules. Real-time polymerase chain reaction (RT-PCR) and Western blot analyses revealed that the thymus had the highest expression of cd8 messenger (m)RNA and protein. After stimulated with phytohaemagglutinin, polyriboinsine-polyribocyaidylic acid and concanavalin A (ConA), the expression level of cd8 mRNA increased significantly in head-kidney lymphocytes at 4 and 8 h, but decreased to normal level at 12 h. Similarly, stimulation with ConA in vivo also led to an increase in the cd8 mRNA level in the spleen. Immunohistochemistry analysis demonstrated that Cd8a-positive cells can be detected in the thymus, spleen and intestine by using polyclonal anti-Cd8a antibody.
英文摘要: The full-length complementary DNA (cDNA) sequences encoding cd8a and cd8 beta molecules were sequenced and characterized from mandarin fish Siniperca chuatsi. Conserved motifs and residues were found to be present in derived peptides of the Cd8 molecules. For example, WXR motif, DXGXYXC motif, and four cysteine residues were present in the extracellular region of the Cd8 protein. Threonine, serine and proline residues involved in multiple O-linked glycosylation events were located in the membrane proximal hinge region. The common CPH motif in the cytoplasmic tail was detected similar to other teleost Cd8 molecules. Different from those in mammals, S. chuatsi Cd8 sequences have many extra cysteine residues (C149 in Cd8a sequence and C46, C51 and C158 in Cd8 beta sequence), which also exist in other teleost Cd8 molecules. Real-time polymerase chain reaction (RT-PCR) and Western blot analyses revealed that the thymus had the highest expression of cd8 messenger (m)RNA and protein. After stimulated with phytohaemagglutinin, polyriboinsine-polyribocyaidylic acid and concanavalin A (ConA), the expression level of cd8 mRNA increased significantly in head-kidney lymphocytes at 4 and 8 h, but decreased to normal level at 12 h. Similarly, stimulation with ConA in vivo also led to an increase in the cd8 mRNA level in the spleen. Immunohistochemistry analysis demonstrated that Cd8a-positive cells can be detected in the thymus, spleen and intestine by using polyclonal anti-Cd8a antibody.
关键词[WOS]: BASS DICENTRARCHUS-LABRAX ; TYROSINE-PROTEIN-KINASE ; CARASSIUS-AURATUS-LANGSDORFII ; CELL-MEDIATED CYTOTOXICITY ; GINBUNA CRUCIAN CARP ; RAINBOW-TROUT ; CYTOPLASMIC DOMAINS ; ONCORHYNCHUS-MYKISS ; CRYSTAL-STRUCTURE ; T-CELLS
语种: 英语
WOS记录号: WOS:000313807000013
ISSN号: 0022-1112
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19311
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Guo, Z; Wang, GL; Fu, JP; Nie, P.Characterization and expression of Cd8 molecules in mandarin fish Siniperca chuatsi,JOURNAL OF FISH BIOLOGY,2013,82(1):189-205
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