IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Characterization and expression of Cd8 molecules in mandarin fish Siniperca chuatsi
Guo, Z.1,2; Wang, G. L.1,2; Fu, J. P.1; Nie, P.1; Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013
Source PublicationJOURNAL OF FISH BIOLOGY
ISSN0022-1112
Volume82Issue:1Pages:189-205
AbstractThe full-length complementary DNA (cDNA) sequences encoding cd8a and cd8 beta molecules were sequenced and characterized from mandarin fish Siniperca chuatsi. Conserved motifs and residues were found to be present in derived peptides of the Cd8 molecules. For example, WXR motif, DXGXYXC motif, and four cysteine residues were present in the extracellular region of the Cd8 protein. Threonine, serine and proline residues involved in multiple O-linked glycosylation events were located in the membrane proximal hinge region. The common CPH motif in the cytoplasmic tail was detected similar to other teleost Cd8 molecules. Different from those in mammals, S. chuatsi Cd8 sequences have many extra cysteine residues (C149 in Cd8a sequence and C46, C51 and C158 in Cd8 beta sequence), which also exist in other teleost Cd8 molecules. Real-time polymerase chain reaction (RT-PCR) and Western blot analyses revealed that the thymus had the highest expression of cd8 messenger (m)RNA and protein. After stimulated with phytohaemagglutinin, polyriboinsine-polyribocyaidylic acid and concanavalin A (ConA), the expression level of cd8 mRNA increased significantly in head-kidney lymphocytes at 4 and 8 h, but decreased to normal level at 12 h. Similarly, stimulation with ConA in vivo also led to an increase in the cd8 mRNA level in the spleen. Immunohistochemistry analysis demonstrated that Cd8a-positive cells can be detected in the thymus, spleen and intestine by using polyclonal anti-Cd8a antibody.; The full-length complementary DNA (cDNA) sequences encoding cd8a and cd8 beta molecules were sequenced and characterized from mandarin fish Siniperca chuatsi. Conserved motifs and residues were found to be present in derived peptides of the Cd8 molecules. For example, WXR motif, DXGXYXC motif, and four cysteine residues were present in the extracellular region of the Cd8 protein. Threonine, serine and proline residues involved in multiple O-linked glycosylation events were located in the membrane proximal hinge region. The common CPH motif in the cytoplasmic tail was detected similar to other teleost Cd8 molecules. Different from those in mammals, S. chuatsi Cd8 sequences have many extra cysteine residues (C149 in Cd8a sequence and C46, C51 and C158 in Cd8 beta sequence), which also exist in other teleost Cd8 molecules. Real-time polymerase chain reaction (RT-PCR) and Western blot analyses revealed that the thymus had the highest expression of cd8 messenger (m)RNA and protein. After stimulated with phytohaemagglutinin, polyriboinsine-polyribocyaidylic acid and concanavalin A (ConA), the expression level of cd8 mRNA increased significantly in head-kidney lymphocytes at 4 and 8 h, but decreased to normal level at 12 h. Similarly, stimulation with ConA in vivo also led to an increase in the cd8 mRNA level in the spleen. Immunohistochemistry analysis demonstrated that Cd8a-positive cells can be detected in the thymus, spleen and intestine by using polyclonal anti-Cd8a antibody.
SubtypeArticle
KeywordCd8a Cd8 Ss Immunohistochemistry Stimulation
Department[Guo, Z. ; Wang, G. L. ; Fu, J. P. ; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Guo, Z. ; Wang, G. L.] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
DOI10.1111/j.1095-8649.2012.03475.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [U0631010]; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000313807000013
WOS KeywordBASS DICENTRARCHUS-LABRAX ; TYROSINE-PROTEIN-KINASE ; CARASSIUS-AURATUS-LANGSDORFII ; CELL-MEDIATED CYTOTOXICITY ; GINBUNA CRUCIAN CARP ; RAINBOW-TROUT ; CYTOPLASMIC DOMAINS ; ONCORHYNCHUS-MYKISS ; CRYSTAL-STRUCTURE ; T-CELLS
Funding OrganizationNational Natural Science Foundation of China [U0631010]; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703] ; National Natural Science Foundation of China [U0631010]; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19311
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Guo, Z.,Wang, G. L.,Fu, J. P.,et al. Characterization and expression of Cd8 molecules in mandarin fish Siniperca chuatsi[J]. JOURNAL OF FISH BIOLOGY,2013,82(1):189-205.
APA Guo, Z.,Wang, G. L.,Fu, J. P.,Nie, P.,&Nie, P .(2013).Characterization and expression of Cd8 molecules in mandarin fish Siniperca chuatsi.JOURNAL OF FISH BIOLOGY,82(1),189-205.
MLA Guo, Z.,et al."Characterization and expression of Cd8 molecules in mandarin fish Siniperca chuatsi".JOURNAL OF FISH BIOLOGY 82.1(2013):189-205.
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