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题名: Zebrafish dmrta2 Regulates the Expression of cdkn2c in Spermatogenesis in the Adult Testis
作者: Xu, Shan1; Xia, Wei2; Zohar, Yonathan2; Gui, Jian-Fang1
通讯作者: Gui, JF (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
关键词: embryo ; fish reproduction ; gametogenesis ; gene regulation ; genomics
刊名: BIOLOGY OF REPRODUCTION
发表日期: 2013
DOI: 10.1095/biolreprod.112.105130
卷: 88, 期:1
收录类别: SCI
文章类型: Article
部门归属: [Xu, Shan ; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; [Xia, Wei ; Zohar, Yonathan] Univ Maryland Baltimore Cty, Dept Marine Biotechnol, Baltimore, MD 21228 USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
类目[WOS]: Reproductive Biology
研究领域[WOS]: Reproductive Biology
摘要: The exact function of the doublesex and mab-3 related transcription factor-like family a2 gene (dmrta2) has remained largely unknown possibly because of its functional redundancy with dmrta1 in most vertebrates. In this study, dmrta1 was demonstrated to likely be absent in the zebrafish genome, which facilitated our functional analysis of dmrta2 in this model organism. To analyze its gene function in embryos and adults, we generated a mutant form of Dmrta2 (R106Q, Dmrta2(RQ)) with its in vitro DNA-binding capacity abolished and a transgenic line for the inducible expression of this mutant Dmrta2(RQ) upon doxycycline (Dox) treatment. Preferential dmrta2 expression was detected in the developing brain during embryogenesis and in the adult testis. During embryogenesis, Dmrta2(RQ) expression caused severe embryonic development defects and dramatic expression changes of two telencephalic marker genes, fibroblast growth factor 8a (fgf8a), and empty spiracles homolog 1 (emx1). In adults, the inducible Dmrta2(RQ) expression occurred specifically in the adult testis and recapitulated the endogenous dmrta2 expression in this organ. Intriguingly, adult males expressing dmrta2(RQ) showed normal spermatogenesis and were fertile, but the expression of cyclin-dependent kinase inhibitor 2C (cdkn2c), which is evolutionarily clustered with dmrta2, was significantly suppressed during spermatogenesis. Further protein-binding and promoter mutation analysis indicated that a putative Dmrta2-binding site on the cdkn2c promoter was required for sustaining the normal expression of cdkn2c during zebrafish spermatogenesis, suggesting that Dmrta2 might regulate the expression of cdkn2c.
英文摘要: The exact function of the doublesex and mab-3 related transcription factor-like family a2 gene (dmrta2) has remained largely unknown possibly because of its functional redundancy with dmrta1 in most vertebrates. In this study, dmrta1 was demonstrated to likely be absent in the zebrafish genome, which facilitated our functional analysis of dmrta2 in this model organism. To analyze its gene function in embryos and adults, we generated a mutant form of Dmrta2 (R106Q, Dmrta2(RQ)) with its in vitro DNA-binding capacity abolished and a transgenic line for the inducible expression of this mutant Dmrta2(RQ) upon doxycycline (Dox) treatment. Preferential dmrta2 expression was detected in the developing brain during embryogenesis and in the adult testis. During embryogenesis, Dmrta2(RQ) expression caused severe embryonic development defects and dramatic expression changes of two telencephalic marker genes, fibroblast growth factor 8a (fgf8a), and empty spiracles homolog 1 (emx1). In adults, the inducible Dmrta2(RQ) expression occurred specifically in the adult testis and recapitulated the endogenous dmrta2 expression in this organ. Intriguingly, adult males expressing dmrta2(RQ) showed normal spermatogenesis and were fertile, but the expression of cyclin-dependent kinase inhibitor 2C (cdkn2c), which is evolutionarily clustered with dmrta2, was significantly suppressed during spermatogenesis. Further protein-binding and promoter mutation analysis indicated that a putative Dmrta2-binding site on the cdkn2c promoter was required for sustaining the normal expression of cdkn2c during zebrafish spermatogenesis, suggesting that Dmrta2 might regulate the expression of cdkn2c.
关键词[WOS]: DM-DOMAIN GENE ; DEPENDENT KINASE INHIBITORS ; SEX DETERMINATION ; RESTRICTED EXPRESSION ; EVOLUTIONARY DYNAMICS ; XENOPUS-LAEVIS ; MEDAKA FISH ; DNA-BINDING ; DOUBLESEX ; MOUSE
语种: 英语
WOS记录号: WOS:000314357700013
ISSN号: 0006-3363
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19310
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Maryland Baltimore Cty, Dept Marine Biotechnol, Baltimore, MD 21228 USA

Recommended Citation:
Xu, S; Xia, W; Zohar, Y; Gui, JF.Zebrafish dmrta2 Regulates the Expression of cdkn2c in Spermatogenesis in the Adult Testis,BIOLOGY OF REPRODUCTION,2013,88(1):
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