IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)
Shi, Yan1; Zhao, Zhe2; Zhu, Xinping1; Chen, Kunci1; Zhang, Qiya3; Zhu, XP (reprint author), Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China.
2013
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume34Issue:1Pages:273-279
AbstractGRIM-19 is a nuclear encoded subunit of complex I that has been implicated in apoptosis. The protein participates in multiple functions including the innate immune response. GRIM-19 has been studied in humans and other mammals; however, fish GRIM-19 has not been well characterized. In this study, a new GRIM-19 gene, EcGRIM-19, was isolated from the orange-spotted grouper (Epinephelus coioides) cDNA library, which was constructed following LPS treatment. EcGRIM-19 is a 582-bp gene that encodes a 144-amino acid protein. The gene is a true ortholog of mammalian GRIM-19. EcGRIM-19 exhibits ubiquitous and constitutive expression in the different tissues of the orange-spotted grouper. The expression levels of EcGRIM-19 are altered in the gill, spleen, kidney and liver after induction with LPS. The subcellular localization analysis demonstrated that the EcGRIM-19 protein is localized predominantly in the mitochondria. In addition, amino acids 30-50 of the protein are responsible for the mitochondrial localization of EcGRIM-19. The caspase assay demonstrated that the overexpression of GRIM-19 enhanced the cellular sensitivity to interferon(IFN)-beta- and retinoic acid (RA)-induced death in HeLa cells. The data presented in this study are important for further understanding the EcGRIM-19 gene function in fish. (c) 2012 Elsevier Ltd. All rights reserved.; GRIM-19 is a nuclear encoded subunit of complex I that has been implicated in apoptosis. The protein participates in multiple functions including the innate immune response. GRIM-19 has been studied in humans and other mammals; however, fish GRIM-19 has not been well characterized. In this study, a new GRIM-19 gene, EcGRIM-19, was isolated from the orange-spotted grouper (Epinephelus coioides) cDNA library, which was constructed following LPS treatment. EcGRIM-19 is a 582-bp gene that encodes a 144-amino acid protein. The gene is a true ortholog of mammalian GRIM-19. EcGRIM-19 exhibits ubiquitous and constitutive expression in the different tissues of the orange-spotted grouper. The expression levels of EcGRIM-19 are altered in the gill, spleen, kidney and liver after induction with LPS. The subcellular localization analysis demonstrated that the EcGRIM-19 protein is localized predominantly in the mitochondria. In addition, amino acids 30-50 of the protein are responsible for the mitochondrial localization of EcGRIM-19. The caspase assay demonstrated that the overexpression of GRIM-19 enhanced the cellular sensitivity to interferon(IFN)-beta- and retinoic acid (RA)-induced death in HeLa cells. The data presented in this study are important for further understanding the EcGRIM-19 gene function in fish. (c) 2012 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordGrim-19 Epinephelus Coioides Tissue Distribution Subcellular Localization Overexpression
Department[Shi, Yan ; Zhu, Xinping ; Chen, Kunci] Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China ; [Zhao, Zhe] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China ; [Zhang, Qiya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.fsi.2012.11.017
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationKey Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences [LMB091006]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]; National Natural Science Foundation of China [41276163, 30901117]; Fundamental Research Funds from CAFS [2012A0403] ; Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences [LMB091006]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]; National Natural Science Foundation of China [41276163, 30901117]; Fundamental Research Funds from CAFS [2012A0403] ; Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences [LMB091006]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]; National Natural Science Foundation of China [41276163, 30901117]; Fundamental Research Funds from CAFS [2012A0403] ; Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences [LMB091006]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]; National Natural Science Foundation of China [41276163, 30901117]; Fundamental Research Funds from CAFS [2012A0403]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000314743600032
WOS KeywordINDUCED CELL-DEATH ; COMPLEX-I ; ACID ; IDENTIFICATION ; REGULATOR ; APOPTOSIS ; INTERACTS ; PROTEIN ; GROWTH ; FISH
Funding OrganizationKey Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences [LMB091006]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]; National Natural Science Foundation of China [41276163, 30901117]; Fundamental Research Funds from CAFS [2012A0403] ; Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences [LMB091006]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]; National Natural Science Foundation of China [41276163, 30901117]; Fundamental Research Funds from CAFS [2012A0403] ; Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences [LMB091006]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]; National Natural Science Foundation of China [41276163, 30901117]; Fundamental Research Funds from CAFS [2012A0403] ; Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences [LMB091006]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]; National Natural Science Foundation of China [41276163, 30901117]; Fundamental Research Funds from CAFS [2012A0403]
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19309
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhu, XP (reprint author), Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China.
Affiliation1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China
2.Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Shi, Yan,Zhao, Zhe,Zhu, Xinping,et al. Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(1):273-279.
APA Shi, Yan,Zhao, Zhe,Zhu, Xinping,Chen, Kunci,Zhang, Qiya,&Zhu, XP .(2013).Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,34(1),273-279.
MLA Shi, Yan,et al."Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 34.1(2013):273-279.
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