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题名: Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model
作者: Liu, Chen-Wei1; Xiong, Feng2; Jia, Hui-Zhen1; Wang, Xu-Li3; Cheng, Han1; Sun, Yong-Hua2; Zhang, Xian-Zheng1; Zhuo, Ren-Xi1; Feng, Jun1
通讯作者: Sun, YH (reprint author), Chinese Acad Sci, Inst Hyrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China.
关键词: DRUG-DELIVERY ; LIPOSOMAL DOXORUBICIN ; PHOTOTHERMAL THERAPY ; CARBON NANOTUBES ; CANCER-THERAPY ; IN-VITRO ; OXIDE ; NANOPARTICLES ; DISCOVERY ; CHEMISTRY
刊名: BIOMACROMOLECULES
发表日期: 2013-02-01
DOI: 10.1021/bm3015297
卷: 14, 期:2, 页:358-366
收录类别: SCI
文章类型: Article
部门归属: [Liu, Chen-Wei ; Jia, Hui-Zhen ; Cheng, Han ; Zhang, Xian-Zheng ; Zhuo, Ren-Xi ; Feng, Jun] Wuhan Univ, Dept Chem, Key Lab Biomed Polymers, Wuhan 430072, Peoples R China ; [Xiong, Feng ; Sun, Yong-Hua] Chinese Acad Sci, Inst Hyrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China ; [Wang, Xu-Li] Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84108 USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者: National Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110]
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Organic ; Polymer Science
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
摘要: In this paper, a facile strategy to develop graphene-based delivery nanosystems for effective drug loading and sustained drug release was proposed and validated. Specifically, biocompatible naphthalene-terminated PEG (NP) and anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading to self-assembled, nanosized complexes. It was found that the oxidation degree of GO had a significant impact on the drug-loading efficiency and the structural stability of nanosystems. Interestingly, the nanoassemblies resulted in more effective cellular entry of DOX in comparison with free DOX or DOX-loaded PEG-polyester micelles at equivalent DOX dose, as demonstrated by confocal microscopy studies. Moreover, the nanoassemblies not only exhibited a sustained drug release pattern without an initial burst release, but also significantly improved the stability of formulations which were resistant to drug leaking even in the presence of strong surfactants such as aromatic sodium benzenesulfonate (SBen) and aliphatic sodium dodecylsulfonate (SDS). In addition, the nanoassemblies without DOX loading showed negligible in vitro cytotoxicity, whereas DOX-loaded counterparts led to considerable toxicity against He La cells. The DOX-mediated cytotoxicity of the graphene-based formulation was around 20 folds lower than that of free DOX, most likely due to the slow DOX release from complexes. A zebrafish model was established to assess the in vivo safety profile of curcumin-loaded nanosystems. The results showed they were able to excrete from the zebrafish body rapidly and had nearly no influence on the zebrafish upgrowth. Those encouraging results may prompt the advance of graphene-based nanotherapeutics for biomedical applications.
英文摘要: In this paper, a facile strategy to develop graphene-based delivery nanosystems for effective drug loading and sustained drug release was proposed and validated. Specifically, biocompatible naphthalene-terminated PEG (NP) and anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading to self-assembled, nanosized complexes. It was found that the oxidation degree of GO had a significant impact on the drug-loading efficiency and the structural stability of nanosystems. Interestingly, the nanoassemblies resulted in more effective cellular entry of DOX in comparison with free DOX or DOX-loaded PEG-polyester micelles at equivalent DOX dose, as demonstrated by confocal microscopy studies. Moreover, the nanoassemblies not only exhibited a sustained drug release pattern without an initial burst release, but also significantly improved the stability of formulations which were resistant to drug leaking even in the presence of strong surfactants such as aromatic sodium benzenesulfonate (SBen) and aliphatic sodium dodecylsulfonate (SDS). In addition, the nanoassemblies without DOX loading showed negligible in vitro cytotoxicity, whereas DOX-loaded counterparts led to considerable toxicity against He La cells. The DOX-mediated cytotoxicity of the graphene-based formulation was around 20 folds lower than that of free DOX, most likely due to the slow DOX release from complexes. A zebrafish model was established to assess the in vivo safety profile of curcumin-loaded nanosystems. The results showed they were able to excrete from the zebrafish body rapidly and had nearly no influence on the zebrafish upgrowth. Those encouraging results may prompt the advance of graphene-based nanotherapeutics for biomedical applications.
关键词[WOS]: DRUG-DELIVERY ; IN-VITRO ; LIPOSOMAL DOXORUBICIN ; PHOTOTHERMAL THERAPY ; CARBON NANOTUBES ; CANCER-THERAPY ; OXIDE ; TOXICITY ; WATER ; NANOPARTICLES
语种: 英语
WOS记录号: WOS:000314908500009
ISSN号: 1525-7797
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19308
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Wuhan Univ, Dept Chem, Key Lab Biomed Polymers, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hyrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China
3.Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84108 USA

Recommended Citation:
Liu, CW; Xiong, F; Jia, HZ; Wang, XL; Cheng, H; Sun, YH; Zhang, XZ; Zhuo, RX; Feng, J.Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model,BIOMACROMOLECULES,2013,14(2):358-366
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