IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model
Liu, Chen-Wei1; Xiong, Feng2; Jia, Hui-Zhen1; Wang, Xu-Li3; Cheng, Han1; Sun, Yong-Hua2; Zhang, Xian-Zheng1; Zhuo, Ren-Xi1; Feng, Jun1; Sun, YH (reprint author), Chinese Acad Sci, Inst Hyrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China.
2013-02-01
Source PublicationBIOMACROMOLECULES
ISSN1525-7797
Volume14Issue:2Pages:358-366
AbstractIn this paper, a facile strategy to develop graphene-based delivery nanosystems for effective drug loading and sustained drug release was proposed and validated. Specifically, biocompatible naphthalene-terminated PEG (NP) and anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading to self-assembled, nanosized complexes. It was found that the oxidation degree of GO had a significant impact on the drug-loading efficiency and the structural stability of nanosystems. Interestingly, the nanoassemblies resulted in more effective cellular entry of DOX in comparison with free DOX or DOX-loaded PEG-polyester micelles at equivalent DOX dose, as demonstrated by confocal microscopy studies. Moreover, the nanoassemblies not only exhibited a sustained drug release pattern without an initial burst release, but also significantly improved the stability of formulations which were resistant to drug leaking even in the presence of strong surfactants such as aromatic sodium benzenesulfonate (SBen) and aliphatic sodium dodecylsulfonate (SDS). In addition, the nanoassemblies without DOX loading showed negligible in vitro cytotoxicity, whereas DOX-loaded counterparts led to considerable toxicity against He La cells. The DOX-mediated cytotoxicity of the graphene-based formulation was around 20 folds lower than that of free DOX, most likely due to the slow DOX release from complexes. A zebrafish model was established to assess the in vivo safety profile of curcumin-loaded nanosystems. The results showed they were able to excrete from the zebrafish body rapidly and had nearly no influence on the zebrafish upgrowth. Those encouraging results may prompt the advance of graphene-based nanotherapeutics for biomedical applications.; In this paper, a facile strategy to develop graphene-based delivery nanosystems for effective drug loading and sustained drug release was proposed and validated. Specifically, biocompatible naphthalene-terminated PEG (NP) and anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading to self-assembled, nanosized complexes. It was found that the oxidation degree of GO had a significant impact on the drug-loading efficiency and the structural stability of nanosystems. Interestingly, the nanoassemblies resulted in more effective cellular entry of DOX in comparison with free DOX or DOX-loaded PEG-polyester micelles at equivalent DOX dose, as demonstrated by confocal microscopy studies. Moreover, the nanoassemblies not only exhibited a sustained drug release pattern without an initial burst release, but also significantly improved the stability of formulations which were resistant to drug leaking even in the presence of strong surfactants such as aromatic sodium benzenesulfonate (SBen) and aliphatic sodium dodecylsulfonate (SDS). In addition, the nanoassemblies without DOX loading showed negligible in vitro cytotoxicity, whereas DOX-loaded counterparts led to considerable toxicity against He La cells. The DOX-mediated cytotoxicity of the graphene-based formulation was around 20 folds lower than that of free DOX, most likely due to the slow DOX release from complexes. A zebrafish model was established to assess the in vivo safety profile of curcumin-loaded nanosystems. The results showed they were able to excrete from the zebrafish body rapidly and had nearly no influence on the zebrafish upgrowth. Those encouraging results may prompt the advance of graphene-based nanotherapeutics for biomedical applications.
SubtypeArticle
KeywordDrug-delivery Liposomal Doxorubicin Photothermal Therapy Carbon Nanotubes Cancer-therapy In-vitro Oxide Nanoparticles Discovery Chemistry
Department[Liu, Chen-Wei ; Jia, Hui-Zhen ; Cheng, Han ; Zhang, Xian-Zheng ; Zhuo, Ren-Xi ; Feng, Jun] Wuhan Univ, Dept Chem, Key Lab Biomed Polymers, Wuhan 430072, Peoples R China ; [Xiong, Feng ; Sun, Yong-Hua] Chinese Acad Sci, Inst Hyrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China ; [Wang, Xu-Li] Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84108 USA
DOI10.1021/bm3015297
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding OrganizationNational Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110] ; National Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110] ; National Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110] ; National Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000314908500009
WOS KeywordDRUG-DELIVERY ; IN-VITRO ; LIPOSOMAL DOXORUBICIN ; PHOTOTHERMAL THERAPY ; CARBON NANOTUBES ; CANCER-THERAPY ; OXIDE ; TOXICITY ; WATER ; NANOPARTICLES
Funding OrganizationNational Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110] ; National Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110] ; National Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110] ; National Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110]
Citation statistics
Cited Times:47[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19308
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorSun, YH (reprint author), Chinese Acad Sci, Inst Hyrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China.
Affiliation1.Wuhan Univ, Dept Chem, Key Lab Biomed Polymers, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hyrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China
3.Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84108 USA
Recommended Citation
GB/T 7714
Liu, Chen-Wei,Xiong, Feng,Jia, Hui-Zhen,et al. Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model[J]. BIOMACROMOLECULES,2013,14(2):358-366.
APA Liu, Chen-Wei.,Xiong, Feng.,Jia, Hui-Zhen.,Wang, Xu-Li.,Cheng, Han.,...&Sun, YH .(2013).Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model.BIOMACROMOLECULES,14(2),358-366.
MLA Liu, Chen-Wei,et al."Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model".BIOMACROMOLECULES 14.2(2013):358-366.
Files in This Item:
File Name/Size DocType Version Access License
Graphene-Based Antic(490KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Chen-Wei]'s Articles
[Xiong, Feng]'s Articles
[Jia, Hui-Zhen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Chen-Wei]'s Articles
[Xiong, Feng]'s Articles
[Jia, Hui-Zhen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Chen-Wei]'s Articles
[Xiong, Feng]'s Articles
[Jia, Hui-Zhen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.