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Development of 134 novel polynucleotide-repeat microsatellite markers in silver carp (Hypophthalmichthys molitrix)
Guo, Wenjie1,2; Yu, Xiaomu1; Tong, Jingou1; Tong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-06-01
Source PublicationCONSERVATION GENETICS RESOURCES
ISSN1877-7252
Volume5Issue:2Pages:525-528
AbstractIn this study, we developed 134 novel polymorphic polynucleotide-repeat microsatellite markers for silver carp (Hypophthalmichthys molitrix) and characterized 40 loci for polymorphism and genetic diversity in a test population. The number of alleles per locus ranged from 3 to 19 (mean 9.4), and the average observed and expected heterozygosities were 0.74-0.76, respectively. Thirty-five loci were highly informative (PIC > 0.5) in silver carp population. After Bonferroni adjustments, no pairs of loci showed evidence of significant linkage disequilibrium, and none of the loci significantly deviated from the Hardy-Weinberg equilibrium. All 134 silver carp microsatellite loci could be successfully amplified in bighead carp (Hypophthalmichthys nobilis) with 65 loci being polymorphic, indicating a high transferability of these microsatellites across species. This set of novel highly polymorphic polynucleotide-repeat microsatellites would be powerful tools for population and conservation genetics studies in silver carp and also in its closely-related bighead carp.; In this study, we developed 134 novel polymorphic polynucleotide-repeat microsatellite markers for silver carp (Hypophthalmichthys molitrix) and characterized 40 loci for polymorphism and genetic diversity in a test population. The number of alleles per locus ranged from 3 to 19 (mean 9.4), and the average observed and expected heterozygosities were 0.74-0.76, respectively. Thirty-five loci were highly informative (PIC > 0.5) in silver carp population. After Bonferroni adjustments, no pairs of loci showed evidence of significant linkage disequilibrium, and none of the loci significantly deviated from the Hardy-Weinberg equilibrium. All 134 silver carp microsatellite loci could be successfully amplified in bighead carp (Hypophthalmichthys nobilis) with 65 loci being polymorphic, indicating a high transferability of these microsatellites across species. This set of novel highly polymorphic polynucleotide-repeat microsatellites would be powerful tools for population and conservation genetics studies in silver carp and also in its closely-related bighead carp.
SubtypeArticle
KeywordSilver Carp Microsatellite Polymorphism Bighead Carp Cross-species Amplification
Department[Guo, Wenjie ; Yu, Xiaomu ; Tong, Jingou] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Guo, Wenjie] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1007/s12686-012-9843-y
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMOA [200903045]; NSFC [31272647]; MOST 973 of China [2010CB126305] ; MOA [200903045]; NSFC [31272647]; MOST 973 of China [2010CB126305] ; MOA [200903045]; NSFC [31272647]; MOST 973 of China [2010CB126305] ; MOA [200903045]; NSFC [31272647]; MOST 973 of China [2010CB126305]
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000318293100055
WOS KeywordGENETIC-LINKAGE MAP ; CONSTRUCTION ; POPULATIONS ; MIDDLE ; RIVER
Funding OrganizationMOA [200903045]; NSFC [31272647]; MOST 973 of China [2010CB126305] ; MOA [200903045]; NSFC [31272647]; MOST 973 of China [2010CB126305] ; MOA [200903045]; NSFC [31272647]; MOST 973 of China [2010CB126305] ; MOA [200903045]; NSFC [31272647]; MOST 973 of China [2010CB126305]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19306
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Guo, Wenjie,Yu, Xiaomu,Tong, Jingou,et al. Development of 134 novel polynucleotide-repeat microsatellite markers in silver carp (Hypophthalmichthys molitrix)[J]. CONSERVATION GENETICS RESOURCES,2013,5(2):525-528.
APA Guo, Wenjie,Yu, Xiaomu,Tong, Jingou,&Tong, JG .(2013).Development of 134 novel polynucleotide-repeat microsatellite markers in silver carp (Hypophthalmichthys molitrix).CONSERVATION GENETICS RESOURCES,5(2),525-528.
MLA Guo, Wenjie,et al."Development of 134 novel polynucleotide-repeat microsatellite markers in silver carp (Hypophthalmichthys molitrix)".CONSERVATION GENETICS RESOURCES 5.2(2013):525-528.
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