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题名: Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels
作者: Li, Guangyu1; Yan, Wei2; Qiao, Qin1; Chen, Jun3; Cai, Fei4; He, Yan1; Zhang, Xuezhen1
通讯作者: Zhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
关键词: Microcystin-LR ; Histopathology ; Proteomic analysis ; Immunotoxicity ; Spleen
刊名: JOURNAL OF PROTEOMICS
发表日期: 2012-12-21
DOI: 10.1016/j.jprot.2012.09.012
卷: 77, 页:383-393
收录类别: SCI
文章类型: Article
部门归属: [Li, Guangyu ; Qiao, Qin ; He, Yan ; Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China ; [Yan, Wei] Hubei Acad Agr Sci, Inst Agr Qual Stand & Technol, Wuhan 430064, Peoples R China ; [Chen, Jun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China ; [Cai, Fei] Xianning Univ, Coll Med, Dept Pharmacol, Xianning 437100, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576]
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: Microcystin-LR (MCLR), released by toxic cyanobacterial blooms, has received worldwide concerns in the past decades for its hepatotoxicity. Recent studies show that microcystins (MCs) can be accumulated in immune organs and exert notable immunotoxicity. In order to better understand cellular responses in immune tissues disrupted by MCLR treatment, this work mainly focuses on the spleen impairments of rats. After a subchronic 50 d exposure (1 or 10 mu g/kg body weight per day), spleen index, MCLR accumulation, histological change and plasma lysozyme activity were detected in MCLR-treated rat. Results indicated that prolonged exposure of MCLR led to toxin accumulation and caused severe damage in spleen of rats, and eventually impaired the immune functions. To further our understanding of the toxic effects of MCLR on the spleen and the mechanisms behind it, a proteomic analysis was performed to determine the global effects of MCLR on splenetic protein levels. In total, 48 proteins were identified and showed a significant increase or decrease in abundance compared to the control after MCLR exposure. These proteins are mainly involved in immune response, oxidative stress, energetic metabolism and the cytoskeleton assembly, indicating that MCLR exerts complex toxic effects in rat spleen and jointly results in immunotoxicity. (C) 2012 Elsevier B.V. All rights reserved.
英文摘要: Microcystin-LR (MCLR), released by toxic cyanobacterial blooms, has received worldwide concerns in the past decades for its hepatotoxicity. Recent studies show that microcystins (MCs) can be accumulated in immune organs and exert notable immunotoxicity. In order to better understand cellular responses in immune tissues disrupted by MCLR treatment, this work mainly focuses on the spleen impairments of rats. After a subchronic 50 d exposure (1 or 10 mu g/kg body weight per day), spleen index, MCLR accumulation, histological change and plasma lysozyme activity were detected in MCLR-treated rat. Results indicated that prolonged exposure of MCLR led to toxin accumulation and caused severe damage in spleen of rats, and eventually impaired the immune functions. To further our understanding of the toxic effects of MCLR on the spleen and the mechanisms behind it, a proteomic analysis was performed to determine the global effects of MCLR on splenetic protein levels. In total, 48 proteins were identified and showed a significant increase or decrease in abundance compared to the control after MCLR exposure. These proteins are mainly involved in immune response, oxidative stress, energetic metabolism and the cytoskeleton assembly, indicating that MCLR exerts complex toxic effects in rat spleen and jointly results in immunotoxicity. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS ; CARP CTENOPHARYNGODON-IDELLA ; HEAT-SHOCK PROTEINS ; PROTEOMIC ANALYSIS ; HEPATOTOXIC MICROCYSTINS ; INTERMEDIATE-FILAMENTS ; IN-SITU ; EXPRESSION ; FISH ; CELLS
语种: 英语
WOS记录号: WOS:000313535900030
ISSN号: 1874-3919
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19296
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Hubei Acad Agr Sci, Inst Agr Qual Stand & Technol, Wuhan 430064, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
4.Xianning Univ, Coll Med, Dept Pharmacol, Xianning 437100, Peoples R China

Recommended Citation:
Li, GY; Yan, W; Qiao, Q; Chen, J; Cai, F; He, Y; Zhang, XZ.Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels,JOURNAL OF PROTEOMICS,2012,77():383-393
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