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Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels
Li, Guangyu1; Yan, Wei2; Qiao, Qin1; Chen, Jun3; Cai, Fei4; He, Yan1; Zhang, Xuezhen1; Zhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
2012-12-21
Source PublicationJOURNAL OF PROTEOMICS
ISSN1874-3919
Volume77Pages:383-393
AbstractMicrocystin-LR (MCLR), released by toxic cyanobacterial blooms, has received worldwide concerns in the past decades for its hepatotoxicity. Recent studies show that microcystins (MCs) can be accumulated in immune organs and exert notable immunotoxicity. In order to better understand cellular responses in immune tissues disrupted by MCLR treatment, this work mainly focuses on the spleen impairments of rats. After a subchronic 50 d exposure (1 or 10 mu g/kg body weight per day), spleen index, MCLR accumulation, histological change and plasma lysozyme activity were detected in MCLR-treated rat. Results indicated that prolonged exposure of MCLR led to toxin accumulation and caused severe damage in spleen of rats, and eventually impaired the immune functions. To further our understanding of the toxic effects of MCLR on the spleen and the mechanisms behind it, a proteomic analysis was performed to determine the global effects of MCLR on splenetic protein levels. In total, 48 proteins were identified and showed a significant increase or decrease in abundance compared to the control after MCLR exposure. These proteins are mainly involved in immune response, oxidative stress, energetic metabolism and the cytoskeleton assembly, indicating that MCLR exerts complex toxic effects in rat spleen and jointly results in immunotoxicity. (C) 2012 Elsevier B.V. All rights reserved.; Microcystin-LR (MCLR), released by toxic cyanobacterial blooms, has received worldwide concerns in the past decades for its hepatotoxicity. Recent studies show that microcystins (MCs) can be accumulated in immune organs and exert notable immunotoxicity. In order to better understand cellular responses in immune tissues disrupted by MCLR treatment, this work mainly focuses on the spleen impairments of rats. After a subchronic 50 d exposure (1 or 10 mu g/kg body weight per day), spleen index, MCLR accumulation, histological change and plasma lysozyme activity were detected in MCLR-treated rat. Results indicated that prolonged exposure of MCLR led to toxin accumulation and caused severe damage in spleen of rats, and eventually impaired the immune functions. To further our understanding of the toxic effects of MCLR on the spleen and the mechanisms behind it, a proteomic analysis was performed to determine the global effects of MCLR on splenetic protein levels. In total, 48 proteins were identified and showed a significant increase or decrease in abundance compared to the control after MCLR exposure. These proteins are mainly involved in immune response, oxidative stress, energetic metabolism and the cytoskeleton assembly, indicating that MCLR exerts complex toxic effects in rat spleen and jointly results in immunotoxicity. (C) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMicrocystin-lr Histopathology Proteomic Analysis Immunotoxicity Spleen
Department[Li, Guangyu ; Qiao, Qin ; He, Yan ; Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China ; [Yan, Wei] Hubei Acad Agr Sci, Inst Agr Qual Stand & Technol, Wuhan 430064, Peoples R China ; [Chen, Jun] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China ; [Cai, Fei] Xianning Univ, Coll Med, Dept Pharmacol, Xianning 437100, Peoples R China
DOI10.1016/j.jprot.2012.09.012
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576] ; National Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576] ; National Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576] ; National Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000313535900030
WOS KeywordHETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS ; CARP CTENOPHARYNGODON-IDELLA ; HEAT-SHOCK PROTEINS ; PROTEOMIC ANALYSIS ; HEPATOTOXIC MICROCYSTINS ; INTERMEDIATE-FILAMENTS ; IN-SITU ; EXPRESSION ; FISH ; CELLS
Funding OrganizationNational Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576] ; National Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576] ; National Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576] ; National Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576]
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Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19296
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorZhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China.
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Hubei Acad Agr Sci, Inst Agr Qual Stand & Technol, Wuhan 430064, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
4.Xianning Univ, Coll Med, Dept Pharmacol, Xianning 437100, Peoples R China
Recommended Citation
GB/T 7714
Li, Guangyu,Yan, Wei,Qiao, Qin,et al. Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels[J]. JOURNAL OF PROTEOMICS,2012,77:383-393.
APA Li, Guangyu.,Yan, Wei.,Qiao, Qin.,Chen, Jun.,Cai, Fei.,...&Zhang, XZ .(2012).Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels.JOURNAL OF PROTEOMICS,77,383-393.
MLA Li, Guangyu,et al."Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels".JOURNAL OF PROTEOMICS 77(2012):383-393.
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