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The suppression of hematopoiesis function in Balb/c mice induced by prolonged exposure of microcystin-LR
Zhou, Wenshan1,2; Zhang, Xuezhen1; Xie, Ping1,2; Liang, Hualei1; Zhang, Xin1,2; Zhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Lion Hill St 1, Wuhan 430070, Peoples R China.
2013-05-23
Source PublicationTOXICOLOGY LETTERS
ISSN0378-4274
Volume219Issue:2Pages:194-201
AbstractMicrocystins (MCs) cause normocytic anemia in patients in a hemodialysis unit in Caruaru, Brazil in 1996, but the underlying mechanisms are still unclear. In the present study, Balb/c mice were intraperitoneally injected with microcystin-LR (MC-LR) at the doses of 0.5, 2 and 8 mu/kg body weight (bw) every 48 h for 30d. After the prolonged exposure of MC-LR, significant decreases of red blood cell count (RBC), hemoglobin (Hb) and hematocrit (Ht) were observed in 2 and 8 mu g/kg bw groups, but erythrocyte mean corpuscular volume (MCV) showed no significant changes. Significantly elevated micronucleus frequency was observed in bone marrow cells (BMCs) in all MC-LR treatments. The proliferation of BMCs significantly declined in both 2 and 8 mu g/kg bw groups. Serum levels of some hematopoietic growth factors significantly changed in 8 mu g/kg bw group, mainly including granulocyte-macrophage (GM-CSF), erythropoietin (EPO), interleukin-3 (IL-3) and TNF-alpha. The transcriptional levels of these 4 genes in BMCs were also significantly changed in 8 mu g/kg bw group. MC-LR exposure significantly increased the apoptosis rates in all MC-LR treatments. The present study indicates prolonged exposure of MC-LR induces normocytic anemia, and the disturbed hematopoietic growth factors and BMCs apoptosis are responsible for this normocytic anemia. (C) 2013 Elsevier Ireland Ltd. All rights reserved.; Microcystins (MCs) cause normocytic anemia in patients in a hemodialysis unit in Caruaru, Brazil in 1996, but the underlying mechanisms are still unclear. In the present study, Balb/c mice were intraperitoneally injected with microcystin-LR (MC-LR) at the doses of 0.5, 2 and 8 mu/kg body weight (bw) every 48 h for 30d. After the prolonged exposure of MC-LR, significant decreases of red blood cell count (RBC), hemoglobin (Hb) and hematocrit (Ht) were observed in 2 and 8 mu g/kg bw groups, but erythrocyte mean corpuscular volume (MCV) showed no significant changes. Significantly elevated micronucleus frequency was observed in bone marrow cells (BMCs) in all MC-LR treatments. The proliferation of BMCs significantly declined in both 2 and 8 mu g/kg bw groups. Serum levels of some hematopoietic growth factors significantly changed in 8 mu g/kg bw group, mainly including granulocyte-macrophage (GM-CSF), erythropoietin (EPO), interleukin-3 (IL-3) and TNF-alpha. The transcriptional levels of these 4 genes in BMCs were also significantly changed in 8 mu g/kg bw group. MC-LR exposure significantly increased the apoptosis rates in all MC-LR treatments. The present study indicates prolonged exposure of MC-LR induces normocytic anemia, and the disturbed hematopoietic growth factors and BMCs apoptosis are responsible for this normocytic anemia. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
SubtypeArticle
KeywordMicrocystin-lr Bone Marrow Cells Micronucleus Apoptosis Hematopoiesis Hematoxicology
Department[Zhou, Wenshan; Zhang, Xuezhen; Xie, Ping; Liang, Hualei; Zhang, Xin] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China; [Zhou, Wenshan; Xie, Ping; Zhang, Xin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
DOI10.1016/j.toxlet.2013.02.011
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160]
Indexed BySCI
Language英语
WOS Research AreaToxicology
WOS SubjectToxicology
WOS IDWOS:000318838300012
WOS KeywordNECROSIS-FACTOR-ALPHA ; BONE-MARROW ; CYANOBACTERIA BLOOM ; MICRONUCLEUS ASSAY ; PERIPHERAL-BLOOD ; PROGENITOR CELLS ; CYTOCHROME-C ; DNA-DAMAGE ; ANEMIA ; PROLIFERATION
Funding OrganizationNational Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160]
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19286
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorZhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Lion Hill St 1, Wuhan 430070, Peoples R China.
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Wenshan,Zhang, Xuezhen,Xie, Ping,et al. The suppression of hematopoiesis function in Balb/c mice induced by prolonged exposure of microcystin-LR[J]. TOXICOLOGY LETTERS,2013,219(2):194-201.
APA Zhou, Wenshan,Zhang, Xuezhen,Xie, Ping,Liang, Hualei,Zhang, Xin,&Zhang, XZ .(2013).The suppression of hematopoiesis function in Balb/c mice induced by prolonged exposure of microcystin-LR.TOXICOLOGY LETTERS,219(2),194-201.
MLA Zhou, Wenshan,et al."The suppression of hematopoiesis function in Balb/c mice induced by prolonged exposure of microcystin-LR".TOXICOLOGY LETTERS 219.2(2013):194-201.
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