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A pilot study on isolation of microsatellite DNA in the Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)
Zheng Jin-Song (Zhengjinsong@ihb.ac.cn); Liao Xiao-Lin; Tong Jin-Gou; Wang Ding (wangd@ihb.ac.cn); Zheng Jin-Song; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2008
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume32Issue:1Pages:19-25
AbstractTo the aim of developing specific microsatellite markers for the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis 72 microsatellite DNA sequences were isolated using an improved FIASCO (Fast Isolation by AFLP of Sequences Containing Repeats) protocol. According to Weber's classification rules, these sequences were divided into three categories: perfect repeat sequences (58.3% of total), imperfect repeat sequences (22.2%), and compound repeat sequences (19.5%). Thirty PCR printer pairs were designed and synthesized, and then screened by a sample panel of 12 random selected Yangtze finless porpoises. As a result, 14 of them were proved to be polymorphic exhibiting 2 to 13 alleles (mean 5.86 alleles/locus). Their average observed heterozygosity and expected heterozygosity were 0.560 and 0.709 respectively. These are the first batch of specific microsatellite markers for the Yangtze finless porpoise, and they should play great role in succedent conservation genetics studies.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18823
Collection期刊论文
Corresponding AuthorZheng Jin-Song; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zheng Jin-Song ,Liao Xiao-Lin,Tong Jin-Gou,et al. A pilot study on isolation of microsatellite DNA in the Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)[J]. Acta Hydrobiologica Sinica,2008,32(1):19-25.
APA Zheng Jin-Song ,Liao Xiao-Lin,Tong Jin-Gou,Wang Ding ,Zheng Jin-Song,&Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).A pilot study on isolation of microsatellite DNA in the Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis).Acta Hydrobiologica Sinica,32(1),19-25.
MLA Zheng Jin-Song ,et al."A pilot study on isolation of microsatellite DNA in the Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)".Acta Hydrobiologica Sinica 32.1(2008):19-25.
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