The Chinese paddlefish(Psephurus gladius(Martens)) named as "the live fossil in the Yangtze River" is the indigenous species in China, which is the biggest freshwater fish in the world. At present, the Chinese paddlefish has been in imminent danger, so it is extremely difficult to gain its fresh specimens. This research got the formalin-fixed tissues of Chinese paddlefish (I I individuals) as experimental materials from the Institute of hydrobiology, Chinese Academy of Science. We have extracted the genomic DNA from the tissues of muscle, heart, fin and liver by optimizing a series of contrastive experiments, and consummated some researches on molecular ecology by microsatellite molecular markers to realize its genetic characteristics.
DNA extraction formalin-fixed tissue of Psephurus gladius and the applicability of microsatellite primers developed from its related species.pdf（739KB）
Yang Zhong (yangzhong8210@tom.com) ; Zhu Bin; Chang Jianbo (jbchang@mail.ihe.ac.cn).DNA extraction formalin-fixed tissue of Psephurus gladius and the applicability of microsatellite primers developed from its related species,Hupo Kexue,2008,20(2):242-250
