Taking Stentor coeruleus for an instance, we have tested single cell PCR for single gene and whole genome. After optimization, we got perfect results. In the single cell PCR for single gene reaction, 40 fresh cells and 40 neutral red stained cells all got 100% success and rich products, and 40 cells kept in the alcohol (95%) for one week got 82.5% success. In the single cell PCR for whole genome reaction, we employed phi 29 DNA Polymerase and random primer for our PCR amplification and got rich products. Using whole genome amplification (10 times diluted) 4 gene loci were amplified, and all of them got objective gene section successfully.