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题名: A preliminary study of single cell PCR in protozoa: take Stentor coeruleus for an instance
作者: Liu Zhixin (lzx20022456@126.com) ; Yu Yuhe (yhyu@ihb.ac.cn)
通讯作者: Liu Zhixin ; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
刊名: Hupo Kexue
发表日期: 2008
卷: 20, 期:5, 页:669-674
收录类别: 其他
英文摘要: Taking Stentor coeruleus for an instance, we have tested single cell PCR for single gene and whole genome. After optimization, we got perfect results. In the single cell PCR for single gene reaction, 40 fresh cells and 40 neutral red stained cells all got 100% success and rich products, and 40 cells kept in the alcohol (95%) for one week got 82.5% success. In the single cell PCR for whole genome reaction, we employed phi 29 DNA Polymerase and random primer for our PCR amplification and got rich products. Using whole genome amplification (10 times diluted) 4 gene loci were amplified, and all of them got objective gene section successfully.
ISSN号: 1003-5427
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18741
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Recommended Citation:
Liu Zhixin (lzx20022456@126.com) ; Yu Yuhe (yhyu@ihb.ac.cn).A preliminary study of single cell PCR in protozoa: take Stentor coeruleus for an instance,Hupo Kexue,2008,20(5):669-674
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