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A preliminary study of single cell PCR in protozoa: take Stentor coeruleus for an instance
Liu Zhixin (lzx20022456@126.com); Yu Yuhe (yhyu@ihb.ac.cn); Liu Zhixin; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2008
Source PublicationHupo Kexue
ISSN1003-5427
Volume20Issue:5Pages:669-674
AbstractTaking Stentor coeruleus for an instance, we have tested single cell PCR for single gene and whole genome. After optimization, we got perfect results. In the single cell PCR for single gene reaction, 40 fresh cells and 40 neutral red stained cells all got 100% success and rich products, and 40 cells kept in the alcohol (95%) for one week got 82.5% success. In the single cell PCR for whole genome reaction, we employed phi 29 DNA Polymerase and random primer for our PCR amplification and got rich products. Using whole genome amplification (10 times diluted) 4 gene loci were amplified, and all of them got objective gene section successfully.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18741
Collection期刊论文
Corresponding AuthorLiu Zhixin; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu Zhixin ,Yu Yuhe ,Liu Zhixin,et al. A preliminary study of single cell PCR in protozoa: take Stentor coeruleus for an instance[J]. Hupo Kexue,2008,20(5):669-674.
APA Liu Zhixin ,Yu Yuhe ,Liu Zhixin,&Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2008).A preliminary study of single cell PCR in protozoa: take Stentor coeruleus for an instance.Hupo Kexue,20(5),669-674.
MLA Liu Zhixin ,et al."A preliminary study of single cell PCR in protozoa: take Stentor coeruleus for an instance".Hupo Kexue 20.5(2008):669-674.
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