Exposure to the external aqueous environment enhances the susceptibility to infectious diseases in fish gills. To understand the mechanism of local innate immunity against infectious grass carp reovirus ( GCRV) in the gills, we identified the partial cDNA sequence of Mx, an antiviral effector molecule, in rare minnow Gobiocypris rarus. Real-time quantitative RT-PCR ( qRT-PCR) was employed to quantitate rnRNA levels of a pattern recognition receptor, Toll-like receptor 3 ( TLR3), and type 1 interferons indicator molecule Mx. The artificial infection experiments revealed that TLR3 and Mx mRNA expressions were simultaneously significant tip-regulation at 12h postinjection ( p 0.05), the transcription level of TLR3 recovered at 48h postinjection (p > 0.05), and the Mx mRNA expression kept at high transcription level till moribund (p < 0.05). These results highlighted the importance of the gills as a tissue capable of mounting a local immune response, and the interferon pathway was activated to GCRV infection.
Su Jian-Guo (firstname.lastname@example.org) ; Zhu Zuo-Yan (email@example.com) ; Wang Ya-Ping.UP-REGULATING EXPRESSIONS OF TOLL-LIKE RECEPTOR 3 AND MX GENES IN GILLS BY GRASS CARP REOVIRUS IN RARE MINNOW, GOBIOCYPRIS RARUS,Acta Hydrobiologica Sinica,2008,32(5):728-734