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题名: ON APOPTOSIS OF HUMAN EPIDERMOID CARCINOMA A431 CELLS INDUCED BY THE EXTRACELLULAR POLYMERIC SUBSTANCES OF SCYTONEMA JAVANICUM
作者: Zhang Hong (zh_elite@126.com) ; Ma Hong-Ying ; Mang De-Lu ; Lu Ying ; Wang Gao-Hong ; Chen Kun ; Liu Yong-Ding ; Hu Chun-Xiang (cxhu@ihb.ac.cn)
通讯作者: Zhang Hong ; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
刊名: Acta Hydrobiologica Sinica
发表日期: 2008
卷: 32, 期:6, 页:874-880
收录类别: 其他
英文摘要: To investigate the effect of extracellular polymeric substances of Scytonema javanicum (EPS) on apoptosis of A431 cells and the expression level of relative genes, cell viability was measured by MTT method, morphologic changes were observed by hematoxylin and eosin staining and transmission electron microscopy, DNA damage was detected by single cell gel electrophoresis (SCGE/comet assay), the expression level of caspase-3, bcl-2 and bax were detected by immunohistochemical staining. EPS significantly inhibited the proliferation of the A431 cells and the inhibitory effect was dose-and time-dependent, which was accompanied by the appearance of morphologic characteristics of apoptosis. The IC50 of A431 cells is 4.25mg/mL after the treatment of EPS for 96h. After 6 mg/mL, EPS treatment for 48h, the DNA damaged seriously in A431 cells when compared with the untreated controls. The expression level of caspase-3 and bax were up-regulation while bcl-2 was down-regulation after 6mg/mL EPS treatment for 72h when compared with the untreated controls respectively. It concluded that EPS may inhibit the proliferation and induce apoptosis of A431 cells, in which caspase-3, bcl-2 and bax were involved.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18723
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Zhang Hong (zh_elite@126.com) ; Ma Hong-Ying; Mang De-Lu; Lu Ying; Wang Gao-Hong; Chen Kun; Liu Yong-Ding; Hu Chun-Xiang (cxhu@ihb.ac.cn).ON APOPTOSIS OF HUMAN EPIDERMOID CARCINOMA A431 CELLS INDUCED BY THE EXTRACELLULAR POLYMERIC SUBSTANCES OF SCYTONEMA JAVANICUM,Acta Hydrobiologica Sinica,2008,32(6):874-880
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文件名: ON APOPTOSIS OF HUMAN EPIDERMOID CARCINOMA A431 CELLS INDUCED BY THE EXTRACELLULAR POLYMERIC SUBSTANCES OF SCYTONEMA JAVANICUM.pdf
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