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题名: Extraction and amplification of genomic DNA from formalin-fixed Coreius heterodon
作者: Wang Dan (wangdan@ihb.ac.cn) ; Tan De-qing ; Tong Jin-gou (jgtong@ihb.ac.cn)
通讯作者: Wang Dan ; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
刊名: Shengtaixue Zazhi
发表日期: 2009
卷: 28, 期:3, 页:572-576
收录类别: 其他
英文摘要: Coreius heterodon is an important commercial fish species in the upper and middle reaches of Yangtze River, and one of the appropriate fishes for the study of possible ecological effects of the Three Gorges Dam. However, the museum C. heterodon specimens were preserved in formalin. It is necessary to approach the methods of effectively extracting the genomic DNA from formalin-fixed C. heterodon, and the feasibility of using the extracted DNA in microsatellite- and mitochondrial analyses. In the present paper, the genomic DNA of ten formalin-fixed C. heterodon specimens was successfully extracted by the steps of removing the formaldehyde through improved gradual dehydration first and extracting the genomic DNA by the traditional phenol-chloroform method then. After primers designed, the PCR amplification was carried out, and a 205 bp of the control region of mtDNA from all of the ten specimens was successfully amplified. SSCP analysis indicated that no polymorphism was detected in this short fragment, and SSR also showed monomorphic. It was suggested that by using alcohol treatment, the formalin-fixed specimens of C. heterodon could be used as the potentially suitable biological materials in the studies of the possible changes of genetic diversity after large hydropower projects were constructed.
ISSN号: 1000-4890
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18705
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Wang Dan (wangdan@ihb.ac.cn) ; Tan De-qing; Tong Jin-gou (jgtong@ihb.ac.cn).Extraction and amplification of genomic DNA from formalin-fixed Coreius heterodon,Shengtaixue Zazhi,2009,28(3):572-576
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文件名: Extraction and amplification of genomic DNA from formalin-fixed Coreius heterodon.pdf
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