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Determination of dehydrogenase activity in sediment of shallow lakes and its ecological significance
Huang Daizhong (hdz1023@163.com); Xiao Wenjuan; Liu Yunbing; Liu Jingyuan; Zhou Yiyong (zhouyy@ihb.ac.cn); Huang Daizhong; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2009
Source PublicationHupo Kexue
ISSN1003-5427
Volume21Issue:3Pages:345-350
AbstractThe samples were taken from the shallow urban lakes(Lake Yuehu) in Wuhan city to optimize the determination of dehydrogenase activity (DHA) in sediment, which gave the following suitable conditions: the sediment sterilized by formaldehyde was used as the control, the concentration of electron acceptor (2,3,5-triphenyl-2H-tetrazolium chloride) was 0.4%, the sediment amount used was 0.5g, pH value was 7.5 and the reaction time was 3h. Under these conditions, the DHA assayed was 3-5 times higher, and its positive relation to the content of organic matter in sediment was more significant, compared to those determined by the unmodified method. The data based on the 23 sites from 3 basins of Lake Donghu demonstrated the feasibility of the method. In the same time, the ecological significance of DHA was further shown in terms of the measurements of microbial activities and organic matter status in lake sediment.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18683
Collection期刊论文
Corresponding AuthorHuang Daizhong; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Huang Daizhong ,Xiao Wenjuan,Liu Yunbing,et al. Determination of dehydrogenase activity in sediment of shallow lakes and its ecological significance[J]. Hupo Kexue,2009,21(3):345-350.
APA Huang Daizhong .,Xiao Wenjuan.,Liu Yunbing.,Liu Jingyuan.,Zhou Yiyong .,...&Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2009).Determination of dehydrogenase activity in sediment of shallow lakes and its ecological significance.Hupo Kexue,21(3),345-350.
MLA Huang Daizhong ,et al."Determination of dehydrogenase activity in sediment of shallow lakes and its ecological significance".Hupo Kexue 21.3(2009):345-350.
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