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EXPRESSED SEQUENCE TAG ANALYSIS OF A 4 YEAR-OLD CHINESE STURGEON PITUITARY
Cao Hong (regancao@ihb.ac.cn); Zhou Li; Gui Jian-Fang (jfgui@ihb.ac.cn); Gui Jian-Fang; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
2009
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume33Issue:4Pages:581-588
AbstractExpressed sequence tag ( EST) analysis is an efficient tool for gene discovery and for profiling gene expression. In order to isolate specific functional genes involved in reproduction and endocrine regulation and to reveal their evolutionary mechanisms in Chinese sturgeon (Acipenser sinensis Gray), a chondrostean fish with a history of 140 million years, we constructed its pituitary cDNA library front a 4 year-old male. A total of 944 random clones were sequenced and compared with sequences in GenBank database. Among all the 944 EST clones, 802 (84.96%) clones were identified as 461 known genes, and additional 142 (15.04%) as unknown genes. Functional categorization indicated that the most abundantly expressed functional gene was the proopiomelanocortin ( POMC), which accounted for almost 10.17% of the overall expression, indicating its important function in the pituitary. Interestingly, the expression patterns of 7 unknown genes were analyzed in various tissues, such as heart, liver, spleen, kidney, muscle, testes, ovary and pituitary. Three different categories of expression patterns were observed from them. Several unknown ESTs, such as EG009334, EG009337, EG009338 and EG009340, were detected to be pituitary-specific, or pituitary and ovary-specific genes. Further studies on their functions will be very useful for better understanding the mechanisms of sturgeon reproduction biology and endocrinology.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18647
Collection期刊论文
Corresponding AuthorGui Jian-Fang; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Cao Hong ,Zhou Li,Gui Jian-Fang ,et al. EXPRESSED SEQUENCE TAG ANALYSIS OF A 4 YEAR-OLD CHINESE STURGEON PITUITARY[J]. Acta Hydrobiologica Sinica,2009,33(4):581-588.
APA Cao Hong ,Zhou Li,Gui Jian-Fang ,Gui Jian-Fang,&Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China.(2009).EXPRESSED SEQUENCE TAG ANALYSIS OF A 4 YEAR-OLD CHINESE STURGEON PITUITARY.Acta Hydrobiologica Sinica,33(4),581-588.
MLA Cao Hong ,et al."EXPRESSED SEQUENCE TAG ANALYSIS OF A 4 YEAR-OLD CHINESE STURGEON PITUITARY".Acta Hydrobiologica Sinica 33.4(2009):581-588.
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