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Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology
Tang Jian-Xin (tangjianxin104@126.com); Chen Zhuo; Hu Han-Hua (hanhuahu@ihb.ac.cn); Tang Jian-Xin; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
2009
Source PublicationYichuan
ISSN0253-9772
Volume31Issue:8Pages:865-870
AbstractSixteen cDNA fragments of Phaeodactylum tricornutum were isolated and identified to be up-regulated under nitrogen starvation by suppression subtractive hybridization (SSH) technology in order to elucidate the molecular mechanism of the diatom nitrogen utilization. Seven of them have high similarity with the functional genes related to the utilization of nitrogen. Northern blotting analysis indicated that 5 genes, nitrate transporter gene (nrt), nitrite reductase gene (nir), ammonium transporter gene (amt), ATP-binding cassette transporter gene (abc), and purine permease gene (pup), were significantly up-regulated under nitrogen starvation.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18639
Collection期刊论文
Corresponding AuthorTang Jian-Xin; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Tang Jian-Xin ,Chen Zhuo,Hu Han-Hua ,et al. Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology[J]. Yichuan,2009,31(8):865-870.
APA Tang Jian-Xin ,Chen Zhuo,Hu Han-Hua ,Tang Jian-Xin,&Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China.(2009).Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology.Yichuan,31(8),865-870.
MLA Tang Jian-Xin ,et al."Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology".Yichuan 31.8(2009):865-870.
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