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题名: Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology
作者: Tang Jian-Xin (tangjianxin104@126.com) ; Chen Zhuo ; Hu Han-Hua (hanhuahu@ihb.ac.cn)
通讯作者: Tang Jian-Xin ; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
刊名: Yichuan
发表日期: 2009
卷: 31, 期:8, 页:865-870
收录类别: 其他
英文摘要: Sixteen cDNA fragments of Phaeodactylum tricornutum were isolated and identified to be up-regulated under nitrogen starvation by suppression subtractive hybridization (SSH) technology in order to elucidate the molecular mechanism of the diatom nitrogen utilization. Seven of them have high similarity with the functional genes related to the utilization of nitrogen. Northern blotting analysis indicated that 5 genes, nitrate transporter gene (nrt), nitrite reductase gene (nir), ammonium transporter gene (amt), ATP-binding cassette transporter gene (abc), and purine permease gene (pup), were significantly up-regulated under nitrogen starvation.
ISSN号: 0253-9772
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18639
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Tang Jian-Xin (tangjianxin104@126.com) ; Chen Zhuo; Hu Han-Hua (hanhuahu@ihb.ac.cn).Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology,Yichuan,2009,31(8):865-870
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文件名: Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology.pdf
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