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题名: STUDY ON SERUM GH EXPRESSION OF TRANSGENIC AND CONTROL COMMON CARP UNDER HUNGRY CONDITION
作者: Zhong Shan (zhongshan@whu.edu.cn) ; Luo Da-Ji (luodaji@whu.edu.cn) ; Wang Ya-Ping (wangyp@ihb.ac.cn) ; Chen Zhu ; Guan Bo ; Liao Lan-Jie ; Zhu Zuo-Yan
通讯作者: Zhong Shan ; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
刊名: Acta Hydrobiologica Sinica
发表日期: 2009
卷: 33, 期:6, 页:1046-1050
收录类别: 其他
英文摘要: This study comparatively analyzed the change regulation of serum growth hormone of transgenic fish and control under starvation and feeding condition by using the Enzyme-linked immunosorbent assays, ELISA technology, and discussed its possible mechanism. The result showed that there was no obviously change in serum growth hormone level of both transgenic fish and the control in feeding experiment, but serum growth hormone level of the former was extremely higher than the latter, which were (142.0 +/- 4.9) ng/mL and (1.6 +/- 0.2) ng/mL respectively, body weight growth rate of the former was also higher than the latter. In starvation experiment, the concentration of serum growth hormone in transgenic fish descended from (142.0 +/- 4.9) ng/mL to (46.0 +/- 3.2) ng/mL rapidly, and then kept at this lower level, while the concentration of serum growth hormone in the control rose up from (1.6 +/- 0.2) ng/mL to (10.9 +/- 1.4) ng/mL continuously,there was no significant difference in body weight minus growth rate between transgenic fish and the control. The study result revealed that the regulatory mechanism of serum growth hormone of transgenic common carp was different with that of the control, because its expression level was not impacted by pituitary feedback inhibition mechanism but related to the control mode of beta-actin gene promoter in the transgene.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18585
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Zhong Shan (zhongshan@whu.edu.cn) ; Luo Da-Ji (luodaji@whu.edu.cn) ; Wang Ya-Ping (wangyp@ihb.ac.cn) ; Chen Zhu; Guan Bo; Liao Lan-Jie; Zhu Zuo-Yan.STUDY ON SERUM GH EXPRESSION OF TRANSGENIC AND CONTROL COMMON CARP UNDER HUNGRY CONDITION,Acta Hydrobiologica Sinica,2009,33(6):1046-1050
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