Improvement of determination on TTC - Dehydrogenase activity in biofilms | |
Li Jin; Wu Zhen-bin; He Feng; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China | |
2005 | |
Source Publication | Jishou Daxue Xuebao (Ziran Kexue Ban)
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ISSN | 1007-2985 |
Volume | 26Issue:1Pages:37-39 |
Abstract | The method of 3, 5, 5 - triphenyltetrazolium (TTC) determining biofilms activity was improved, and the problem that the standard curve of determination on TTC - dehydrogenase activity can't be made conveniently and stably was resolved. The factors which influence the determination were studied. The results showed that sodium sulfide as reducer, toluene as TF organic extractant, Tris - HCl as buffer solution, pH 8.6 and 38 degrees C were the best condition on the developed method, and the optimum reaction Lime to cultivate biofilms is 6 h. |
Indexed By | 其他 |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/18575 |
Collection | 期刊论文 |
Corresponding Author | Li Jin; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China |
Recommended Citation GB/T 7714 | Li Jin,Wu Zhen-bin,He Feng,et al. Improvement of determination on TTC - Dehydrogenase activity in biofilms[J]. Jishou Daxue Xuebao (Ziran Kexue Ban),2005,26(1):37-39. |
APA | Li Jin,Wu Zhen-bin,He Feng,&Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China.(2005).Improvement of determination on TTC - Dehydrogenase activity in biofilms.Jishou Daxue Xuebao (Ziran Kexue Ban),26(1),37-39. |
MLA | Li Jin,et al."Improvement of determination on TTC - Dehydrogenase activity in biofilms".Jishou Daxue Xuebao (Ziran Kexue Ban) 26.1(2005):37-39. |
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