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Genetic diversity analysis of Chinese three-keeled pond turtle (Chinemys reevesii) by RAPD
Zhu Xin-Pin (xinpingzhu@tom.com); Du He-Jun; Zhou Li; Li Ming-You; Gui Jian-Fang (jfgui@ihb.ac.cn); Zhu Xin-Pin; Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan 430072, Peoples R China
2005
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume29Issue:2Pages:167-171
AbstractChinese three-keeled pond turtle (Chinemys reevesii Gray) is a member cf the family Emydidae and widely ranges in southern China, especially in the regions of the lower and middle Yangtze River. It has high values for food, traditional Chinese P medicine and pet trade, and is one of the turtles for aquaculture in China. But the genetic background of Chinese three-keeled pond turtle is still not very clear. In order to realize its population genetic structure and the diversity for the conservation of its germ plasm and prevention of gene loss, genetic diversity analysis was carried out using RAPD-PCR technique.In this paper, genetic diversity of 24 individuals of Chinese three-keeled pond turtle from Hubei province was analyzed by the random amplified polymorphic DNA (RAPD) technique, using 20 10-nucleotide-long primers. Twenty primers produced 3288 well-amplified DNA fragments with reproducible banding patterns and averaged 137 DNA fragments in one individual. 119 of 137 loci detected are polymorphic, amounting to 86.9%. The DNA fragments were from 0.2kb to 3kb. The genetic distance between two individuals was from 0.168 to 0.467. The average genetic distance was 0.324 +/- 0.0631 among the 24 individuals. The phylogenetic tree of the 24 individuals was obtained by NJTREE analysis in the RAPDistance program and the 24 individuals were divided into several clusters and it indicated the turtle maybe have two populations at least. In conclusion, genetic diversity of Chinese three-keeled pond turtle was high.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18547
Collection期刊论文
Corresponding AuthorZhu Xin-Pin; Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhu Xin-Pin ,Du He-Jun,Zhou Li,et al. Genetic diversity analysis of Chinese three-keeled pond turtle (Chinemys reevesii) by RAPD[J]. Acta Hydrobiologica Sinica,2005,29(2):167-171.
APA Zhu Xin-Pin .,Du He-Jun.,Zhou Li.,Li Ming-You.,Gui Jian-Fang .,...&Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2005).Genetic diversity analysis of Chinese three-keeled pond turtle (Chinemys reevesii) by RAPD.Acta Hydrobiologica Sinica,29(2),167-171.
MLA Zhu Xin-Pin ,et al."Genetic diversity analysis of Chinese three-keeled pond turtle (Chinemys reevesii) by RAPD".Acta Hydrobiologica Sinica 29.2(2005):167-171.
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