中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: Intestinal pathology of the mandarin fish Siniperca chuatsi infected naturally with the acanthocephalan Hebesomea violentum
作者: Xie Hai-Xia ; Gao Qian ; Nie Pin (pinnie@ihb.ac.cn)
通讯作者: Xie Hai-Xia ; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
刊名: Acta Hydrobiologica Sinica
发表日期: 2005
卷: 29, 期:2, 页:137-141
收录类别: 其他
英文摘要: Siniperca chuatsi carrying heavy infections of Hebesomea Violentum in the gut were obtained from Liangzi Lake, Hubei Province. For light microscopical examination, the gut were fixed overnight in MFAA (Mathanol : Formalin: Acetic acid = 85: 10: 5). After embedding in Histowax, the tissues were serially sectioned at Stint and stained with May-Grunwald Giemsa using standard techniques. For transmission electronic microscopical examination, small pieces of gut with attached parasites were immersed in ice-cooled fixative and minced in approximately 2mm(3) pieces and then they were fixed in 2.5% glutaraldehyde in phosphate buffered saline (PBS; 0.1 M; pH 7.2) for 4 hours at 4 degrees C and postfixed for 1h in 1% osmium tetroxide in the same buffer at 4 degrees C, and embedded in Epson-812. Semithin sections were stained with 1% aqueous solution of toluidine blue to select the most suitable areas. Ultrathin sections were double-stained with uranil acetate and lead citrate, and observed in a JEOL JEM-1230 electron microscope at 80kV. The parasite-induced histopathology was related to the penetration of H. violentum proboscis in the intestine wall. The host responded to the parasites with connective tissue hyperplasia and with an infiltration of leukocytes, predominantly eosinophilic granulocytes. Eosinophils were accumulated in the epithelium and lamina propria of the infected gut and some adhere to the parasite tegument and proboscis attachment. These eosinophils were subdivided into immature eosinophils, mature eosinophils and degranulating eosinophils. Fibre cells, fibroblast cells, and eosinophils were orderly distributed at the interface of the parasite-host. In addition, H. violentum elicits the formation of nodule in tunica propria. These nodules comprised a tri-layered structure to wrap the H. violentum. Tissue cava infiltrated by host cells were observed in the lamina propria and they are presumed to be the degenerated nodules.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18543
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Intestinal pathology of the mandarin fish Siniperca chuatsi infected naturally with the acanthocephalan Hebesomea violentum.pdf(217KB)----开放获取--View Download

Recommended Citation:
Xie Hai-Xia; Gao Qian; Nie Pin (pinnie@ihb.ac.cn).Intestinal pathology of the mandarin fish Siniperca chuatsi infected naturally with the acanthocephalan Hebesomea violentum,Acta Hydrobiologica Sinica,2005,29(2):137-141
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xie Hai-Xia]'s Articles
[Gao Qian]'s Articles
[Nie Pin (pinnie@ihb.ac.cn)]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xie Hai-Xia]‘s Articles
[Gao Qian]‘s Articles
[Nie Pin (pinnie@ihb.ac.cn)]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Intestinal pathology of the mandarin fish Siniperca chuatsi infected naturally with the acanthocephalan Hebesomea violentum.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace