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题名: Screen of differentially expressed genes between Gastrula embryos and tail budembryos in gynogenetic gibel carp (Carassius auratus gibello)
作者: Liu Jun ; Shi Yao-Hua ; Yin Jun ; Gui Jian-Fang (jfgui@ihb.ac.cn)
通讯作者: Liu Jun ; Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
刊名: Acta Genetica Sinica
发表日期: 2005
卷: 32, 期:3, 页:253-263
收录类别: 其他
英文摘要: Suppression subtractive hybridization (SSH) cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp ( Carassius auratus gibelio). 739 and 816 PCR positive clones were respectively selected to perform dot blot, and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos. Sequencing analysis and database searches indicated that there were 19 known genes and 31 unknown cDNA fragments in the sequenced 72 dot blot positive clones specific for gastrula embryos,And 52 known genes and 37 unknown cDNA fragments in the sequenced 98 dot blot positive clones specific for tail bud embryos. Moreover,specific expressions of partial genes were further confirmed by virtual Northern blots and RT-PCR. The screen of these differentially expressed genes will help us to understand the molecular mechanism in gibel carp embryogenesis.
ISSN号: 0379-4172
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18535
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Recommended Citation:
Liu Jun; Shi Yao-Hua; Yin Jun; Gui Jian-Fang (jfgui@ihb.ac.cn).Screen of differentially expressed genes between Gastrula embryos and tail budembryos in gynogenetic gibel carp (Carassius auratus gibello),Acta Genetica Sinica,2005,32(3):253-263
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文件名: Screen of differentially expressed genes between Gastrula embryos and tail budembryos in gynogenetic gibel carp (Carassius auratus gibello).pdf
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