中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: Construction of a subtractive cDNA library from the Paralichthys olivaceus embryonic cells induced by a double-stranded RNA virus
作者: Chen Yu-dong ; Zhang Yi-bing ; Zhu Rong ; Jiang Jun ; Zhang Qi-ya ; Gui Jian-fang (jfgui@ihb.ac.cn)
通讯作者: Gui Jian-fang ; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
刊名: Virologica Sinica
发表日期: 2005
卷: 20, 期:2, 页:168-172
收录类别: 其他
英文摘要: Using suppression subtractive hybridization (SSH) technique, a subtractive cDNA library was constructed from Japanese flounder (Paralichthys olivaceus) embryonic cells (FEC) induced by UV-inactivated dsRNA virus GCHV (Grass carp hemorrhage virus). A housekeeping gene. alpha-tublin, was used to estimate the efficiency of subtractive cDNA. In this library alpha-tublin was subtracted at about 2(10) folds, indicating that some differentially expressed genes were also enriched at about the same folds. The length of the subtractive cDNA fragments cloned into pGEM-T vector ranged from 250bp to 20001)p. The results showed that the subtractive cDNA library is successful, which will be very useful for the understanding of antiviral immune response to dsRNA virus and essential for rapid isolation of differentially expressed genes induced by dsRNA virus.
ISSN号: 1674-0769
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18533
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Construction of a subtractive cDNA library from the Paralichthys olivaceus embryonic cells induced by a double-stranded RNA virus.pdf(212KB)----开放获取--View Download

Recommended Citation:
Chen Yu-dong; Zhang Yi-bing; Zhu Rong; Jiang Jun; Zhang Qi-ya; Gui Jian-fang (jfgui@ihb.ac.cn).Construction of a subtractive cDNA library from the Paralichthys olivaceus embryonic cells induced by a double-stranded RNA virus,Virologica Sinica,2005,20(2):168-172
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen Yu-dong]'s Articles
[Zhang Yi-bing]'s Articles
[Zhu Rong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen Yu-dong]‘s Articles
[Zhang Yi-bing]‘s Articles
[Zhu Rong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Construction of a subtractive cDNA library from the Paralichthys olivaceus embryonic cells induced by a double-stranded RNA virus.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace