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Construction of a subtractive cDNA library from the Paralichthys olivaceus embryonic cells induced by a double-stranded RNA virus
Chen Yu-dong; Zhang Yi-bing; Zhu Rong; Jiang Jun; Zhang Qi-ya; Gui Jian-fang (jfgui@ihb.ac.cn); Gui Jian-fang; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
2005
Source PublicationVirologica Sinica
ISSN1674-0769
Volume20Issue:2Pages:168-172
AbstractUsing suppression subtractive hybridization (SSH) technique, a subtractive cDNA library was constructed from Japanese flounder (Paralichthys olivaceus) embryonic cells (FEC) induced by UV-inactivated dsRNA virus GCHV (Grass carp hemorrhage virus). A housekeeping gene. alpha-tublin, was used to estimate the efficiency of subtractive cDNA. In this library alpha-tublin was subtracted at about 2(10) folds, indicating that some differentially expressed genes were also enriched at about the same folds. The length of the subtractive cDNA fragments cloned into pGEM-T vector ranged from 250bp to 20001)p. The results showed that the subtractive cDNA library is successful, which will be very useful for the understanding of antiviral immune response to dsRNA virus and essential for rapid isolation of differentially expressed genes induced by dsRNA virus.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18533
Collection期刊论文
Corresponding AuthorGui Jian-fang; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen Yu-dong,Zhang Yi-bing,Zhu Rong,et al. Construction of a subtractive cDNA library from the Paralichthys olivaceus embryonic cells induced by a double-stranded RNA virus[J]. Virologica Sinica,2005,20(2):168-172.
APA Chen Yu-dong.,Zhang Yi-bing.,Zhu Rong.,Jiang Jun.,Zhang Qi-ya.,...&Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China.(2005).Construction of a subtractive cDNA library from the Paralichthys olivaceus embryonic cells induced by a double-stranded RNA virus.Virologica Sinica,20(2),168-172.
MLA Chen Yu-dong,et al."Construction of a subtractive cDNA library from the Paralichthys olivaceus embryonic cells induced by a double-stranded RNA virus".Virologica Sinica 20.2(2005):168-172.
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