中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: Expression characterization of ZP3 gene and isolation of chorion ZP3 protein in Carassius auratus gibelio
作者: Chen Bo ; Guo Wei ; Gui Jian-Fang (jfgui@ihb.ac.cn)
通讯作者: Chen Bo ; Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol and Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
刊名: Acta Hydrobiologica Sinica
发表日期: 2005
卷: 29, 期:3, 页:233-238
收录类别: 其他
英文摘要: Full length ZP3 cDNA had been cloned from Carassius auratus gibelio. In this study, the ZP3 and GST recombinant protein was produced by constructing the pGEX-KG expression vector and transforming the bacterium DE3 strain, and the antibody against the ZP3 recombinant protein was prepared from the immunized rabbit. Furthermore, expression characterization and status changes of ZP3 protein during oocyte maturation and early embryogenesis were analyzed by RT-PCR and Western blot. The data indicated that the ZP3 transcription was initiated before previtellogenic stage, while the ZP3 protein was translated from the stage of vitellogenesis, and the amount reached to its peak in the fully-grown oocytes. Significant changes of ZP3 status occurred after egg fertilization and early embryogenesis. After 30 minutes of post-fertilization, the original ZP3 protein disappeared from the extract, instead of that, the ZP3 antibody recognized bigger protein bands, and all of them could not be detected from the extract after 8-16 cell stage of embryogenesis. It implicated that the ZP3 dimeric complex and polymerized complexes were formed after egg fertilization and early embryogenesis. Moreover, the chorion proteins were separated by 12% SDS-PAGE and stained by Coomassie brilliant blue. Eight protein bands were respectively excised and recovered from the gel. The recovered proteins were again separated by 12% SDS-PAGE, and transferred to nitrocellulose membrane. Then they were detected by Western blot with the anti-ZP3 serum. The result indicated that the fifth protein band was positive ZP3 protein with about 50 KD and the second protein band is also positive, but the molecular weight is higher than that of ZP3, which may be the complex of ZP3 and other protein. Therefore, we purified ZP3 protein of Carassius auratus gibelio from the isolated chorion by SDS-PAGE separation and Western blot detection.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18523
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Expression characterization of ZP3 gene and isolation of chorion ZP3 protein in Carassius auratus gibelio.pdf(228KB)----开放获取--View Download

Recommended Citation:
Chen Bo; Guo Wei; Gui Jian-Fang (jfgui@ihb.ac.cn).Expression characterization of ZP3 gene and isolation of chorion ZP3 protein in Carassius auratus gibelio,Acta Hydrobiologica Sinica,2005,29(3):233-238
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen Bo]'s Articles
[Guo Wei]'s Articles
[Gui Jian-Fang (jfgui@ihb.ac.cn)]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen Bo]‘s Articles
[Guo Wei]‘s Articles
[Gui Jian-Fang (jfgui@ihb.ac.cn)]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Expression characterization of ZP3 gene and isolation of chorion ZP3 protein in Carassius auratus gibelio.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace