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Binding of the Z alpha domain from a Carassius auratus protein kinase PKR-like to polyinosinic:polycytidylic acid
Hu Cheng-yu; Xie Zong-bo; Zhang Yi-bing; Chen Yu-dong; Deng Zheng-dong; Jiang Jun; Gui Jian-fang (jfgui@ihb.ac.cn); Gui Jian-fang; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
2005
Source PublicationZoological Research
ISSN0254-5853
Volume26Issue:3Pages:237-242
AbstractThree fusion peptides, P-Z alpha 1, P-Z alpha 2 and P-Z alpha 1Z alpha 2 for Z alpha 1 domain, Z alpha 2 domain, and Z alpha 1Z alpha 2 domains of Carassius auratus PKR-like gene, respectively, were successfully expressed by a prokaryotic expression system and then purified by affinity chromatography. Gel mobility shift assay revealed that P-Z alpha 1Z alpha 2 rather than P-Z alpha 1, P-Z alpha 2, and mixture of P-Z alpha 1 and P-Z alpha 2, was capable to bind to polyinosinic: polycytidylic acid (Poly 1: C) in vitro. In addition, all of the three fusion peptides all could form dimer, with strong dimerization for P-Z alpha 2 and P-Z alpha 1Z alpha 2 but a relative weak one for P-Z alpha 1. The results suggest that dsRNA, the by-product generated during virus replication in host cells, probably binds to the Z alpha domain of CaPKR-like and then regulates its physiological function.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18519
Collection期刊论文
Corresponding AuthorGui Jian-fang; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Hu Cheng-yu,Xie Zong-bo,Zhang Yi-bing,et al. Binding of the Z alpha domain from a Carassius auratus protein kinase PKR-like to polyinosinic:polycytidylic acid[J]. Zoological Research,2005,26(3):237-242.
APA Hu Cheng-yu.,Xie Zong-bo.,Zhang Yi-bing.,Chen Yu-dong.,Deng Zheng-dong.,...&Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China.(2005).Binding of the Z alpha domain from a Carassius auratus protein kinase PKR-like to polyinosinic:polycytidylic acid.Zoological Research,26(3),237-242.
MLA Hu Cheng-yu,et al."Binding of the Z alpha domain from a Carassius auratus protein kinase PKR-like to polyinosinic:polycytidylic acid".Zoological Research 26.3(2005):237-242.
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