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题名: Binding of the Z alpha domain from a Carassius auratus protein kinase PKR-like to polyinosinic:polycytidylic acid
作者: Hu Cheng-yu ; Xie Zong-bo ; Zhang Yi-bing ; Chen Yu-dong ; Deng Zheng-dong ; Jiang Jun ; Gui Jian-fang (jfgui@ihb.ac.cn)
通讯作者: Gui Jian-fang ; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
刊名: Zoological Research
发表日期: 2005
卷: 26, 期:3, 页:237-242
收录类别: 其他
英文摘要: Three fusion peptides, P-Z alpha 1, P-Z alpha 2 and P-Z alpha 1Z alpha 2 for Z alpha 1 domain, Z alpha 2 domain, and Z alpha 1Z alpha 2 domains of Carassius auratus PKR-like gene, respectively, were successfully expressed by a prokaryotic expression system and then purified by affinity chromatography. Gel mobility shift assay revealed that P-Z alpha 1Z alpha 2 rather than P-Z alpha 1, P-Z alpha 2, and mixture of P-Z alpha 1 and P-Z alpha 2, was capable to bind to polyinosinic: polycytidylic acid (Poly 1: C) in vitro. In addition, all of the three fusion peptides all could form dimer, with strong dimerization for P-Z alpha 2 and P-Z alpha 1Z alpha 2 but a relative weak one for P-Z alpha 1. The results suggest that dsRNA, the by-product generated during virus replication in host cells, probably binds to the Z alpha domain of CaPKR-like and then regulates its physiological function.
ISSN号: 0254-5853
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18519
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Hu Cheng-yu; Xie Zong-bo; Zhang Yi-bing; Chen Yu-dong; Deng Zheng-dong; Jiang Jun; Gui Jian-fang (jfgui@ihb.ac.cn).Binding of the Z alpha domain from a Carassius auratus protein kinase PKR-like to polyinosinic:polycytidylic acid,Zoological Research,2005,26(3):237-242
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