IHB OpenIR  > 期刊论文
Screening of diefferentially expressed genes at gastrula stage during embryogenesis of Carassius auratus gibelio
Liu Jing-Xia; Shi Yao-Hua; Gui Jian-Fang (jfgui@ihb.ac.cn); Chinese Acad Sci, Inst Hydrobiol, State Key lab Freshwater Ecol and Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
2005
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume29Issue:4Pages:359-365
AbstractEmbryogenesis is a progressive and complicated process that is regulated by differential gene activity. In order to identify some differentially expressed genes in early embryogenesis, two kinds of SMART cDNAs were respectively synthesized from mature eggs and gastrula embryos of Carassius auratus gibelio, and the gastrula embryo SMART cDNA library was constructed. Following this program, 131 positive clones were screened from about 1500 clones of the gastrula embryo SMART cDNA library by two rounds of dot-blot differential hybridizations using the synthesized SMART cDNAs as probes. Furthermore, 58 positive clones were selected for sequencing. Searching GenBank by the nucleotide sequences indicated that most of the sequenced cDNAs encoded ribosome proteins and regulators involved in translation and transcription, and some encoded lipoproteins involved in the metabolism of fat. And, some unknown new genes were also identified from the screening. Moreover, we investigated the expression patterns of 6 genes during embryogenesis by RT-PCR analysis, and confirmed their differential expression during embryogenesis. Four different expression patterns, such as transcribing again at gastrula based on the maternal products, beginning to transcribe at blastula stage, beginning to transcribe at gastrula stage, and transcribing only during embryogenesis, were revealed in the analyzed genes. Through this study, we cloned and identified some genes expressed differentially at early stage during embryogenesis, which will be a solid base for further researches on their functions and regulative mechanisms during embryogenesis.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18503
Collection期刊论文
Corresponding AuthorLiu Jing-Xia; Chinese Acad Sci, Inst Hydrobiol, State Key lab Freshwater Ecol and Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu Jing-Xia,Shi Yao-Hua,Gui Jian-Fang ,et al. Screening of diefferentially expressed genes at gastrula stage during embryogenesis of Carassius auratus gibelio[J]. Acta Hydrobiologica Sinica,2005,29(4):359-365.
APA Liu Jing-Xia,Shi Yao-Hua,Gui Jian-Fang ,&Chinese Acad Sci, Inst Hydrobiol, State Key lab Freshwater Ecol and Biotechnol, Grad Sch, Wuhan 430072, Peoples R China.(2005).Screening of diefferentially expressed genes at gastrula stage during embryogenesis of Carassius auratus gibelio.Acta Hydrobiologica Sinica,29(4),359-365.
MLA Liu Jing-Xia,et al."Screening of diefferentially expressed genes at gastrula stage during embryogenesis of Carassius auratus gibelio".Acta Hydrobiologica Sinica 29.4(2005):359-365.
Files in This Item:
File Name/Size DocType Version Access License
Screening of dieffer(264KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu Jing-Xia]'s Articles
[Shi Yao-Hua]'s Articles
[Gui Jian-Fang (jfgui@ihb.ac.cn)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu Jing-Xia]'s Articles
[Shi Yao-Hua]'s Articles
[Gui Jian-Fang (jfgui@ihb.ac.cn)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu Jing-Xia]'s Articles
[Shi Yao-Hua]'s Articles
[Gui Jian-Fang (jfgui@ihb.ac.cn)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Screening of diefferentially expressed genes at gastrula stage during embryogenesis of Carassius auratus gibelio.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.