Genetic relationships of five ciliate strains inferred from ISSR analysis | |
Zhang Wen-Jing; Yu Yu-He (yhyu@ihb.ac.cn); Shen Yun-Fen; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China | |
2005 | |
Source Publication | Acta Hydrobiologica Sinica
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ISSN | 1000-3207 |
Volume | 29Issue:6Pages:633-638 |
Abstract | ISSR analysis was conducted to investigate the genctic relationships of five ciliate strains: Epistylis plicatilis, Paramecium bursaria, Stentor polymorphus, Tetrahymena thermophila BF1 and T. thermophila BF5. Out of 13 ISSR primers, 6 primers generated highly reproducible and polymorphic DNA fragments. The genetic trees were constructed based on ISSR amplification fingerprintings using both unweighted pair-group method for arithmetic averages analysis (UPGMA) and neighbor-joining (NJ) methods. Two T. thermophila strains formed one branch first. E. Plicatilis clustered with S. polymorphus then grouped with T. thermophila. P. bursaria formed a separate branch. This shows that (1) the peritrichs may he recognized as the particular ciliates within Oligohymenophorea, and they should be elevaled to a higher taxonomic rank; (2) the Peritrichia E. plicatilis has the closer relation with the Hymenostomatia T. thermophila than the Peniculia P. bursaria, which is the primordial group in the class Oligohymenophorea; (3) The genome of the five ciliate strains contain these microsatellite DNA sequences: (GTC)(4), (GACA)(4), (AG)(8), (CAA)(6) and (GAA)(6). |
Indexed By | 其他 |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/18451 |
Collection | 期刊论文 |
Corresponding Author | Zhang Wen-Jing; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
Recommended Citation GB/T 7714 | Zhang Wen-Jing,Yu Yu-He ,Shen Yun-Fen,et al. Genetic relationships of five ciliate strains inferred from ISSR analysis[J]. Acta Hydrobiologica Sinica,2005,29(6):633-638. |
APA | Zhang Wen-Jing,Yu Yu-He ,Shen Yun-Fen,&Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2005).Genetic relationships of five ciliate strains inferred from ISSR analysis.Acta Hydrobiologica Sinica,29(6),633-638. |
MLA | Zhang Wen-Jing,et al."Genetic relationships of five ciliate strains inferred from ISSR analysis".Acta Hydrobiologica Sinica 29.6(2005):633-638. |
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