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Analysis and expression of tumor necrosis factor receptor homolog from LCDV-C
Huang You-hua; Sun Wei; Zhang Qi-ya (zhangqy@ihb.ac.cn); Zhang Qi-ya; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
2005
Source PublicationVirologica Sinica
ISSN1674-0769
Volume20Issue:6Pages:652-655
AbstractTumor necrosis factor receptor (TNFR) plays an important role in the evasion of immune response by large DNA viruses. TNFR is a homologue of cellular receptors. Lymphocystis disease virus, an iriduvirus, isolated in China (LCDV-C) is a large DNA virus. Primers were de signed based on conserved TNFR nucleotide sequences in iridoviruses and used to amplify the homologue in LCDV-C. A DNA fragment of 834 bp was generated and sequenced. The recombinant prokaryotic expression plasmid containing this fragment was constructed and expressed in E. coli. DE3. An expected 45kDa fusion protein was isolated by SDS-PAGE. Computer analysis indicated that LCDV-C TNFR homologue encodes a 278aa putative protein, which contains a typical cysteine-rich domain. It shares 34% identity with flounder TNFRII.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18447
Collection期刊论文
Corresponding AuthorZhang Qi-ya; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Huang You-hua,Sun Wei,Zhang Qi-ya ,et al. Analysis and expression of tumor necrosis factor receptor homolog from LCDV-C[J]. Virologica Sinica,2005,20(6):652-655.
APA Huang You-hua,Sun Wei,Zhang Qi-ya ,Zhang Qi-ya,&Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China.(2005).Analysis and expression of tumor necrosis factor receptor homolog from LCDV-C.Virologica Sinica,20(6),652-655.
MLA Huang You-hua,et al."Analysis and expression of tumor necrosis factor receptor homolog from LCDV-C".Virologica Sinica 20.6(2005):652-655.
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