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题名: Analysis and expression of tumor necrosis factor receptor homolog from LCDV-C
作者: Huang You-hua ; Sun Wei ; Zhang Qi-ya (zhangqy@ihb.ac.cn)
通讯作者: Zhang Qi-ya ; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
刊名: Virologica Sinica
发表日期: 2005
卷: 20, 期:6, 页:652-655
收录类别: 其他
英文摘要: Tumor necrosis factor receptor (TNFR) plays an important role in the evasion of immune response by large DNA viruses. TNFR is a homologue of cellular receptors. Lymphocystis disease virus, an iriduvirus, isolated in China (LCDV-C) is a large DNA virus. Primers were de signed based on conserved TNFR nucleotide sequences in iridoviruses and used to amplify the homologue in LCDV-C. A DNA fragment of 834 bp was generated and sequenced. The recombinant prokaryotic expression plasmid containing this fragment was constructed and expressed in E. coli. DE3. An expected 45kDa fusion protein was isolated by SDS-PAGE. Computer analysis indicated that LCDV-C TNFR homologue encodes a 278aa putative protein, which contains a typical cysteine-rich domain. It shares 34% identity with flounder TNFRII.
ISSN号: 1674-0769
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18447
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Huang You-hua; Sun Wei; Zhang Qi-ya (zhangqy@ihb.ac.cn).Analysis and expression of tumor necrosis factor receptor homolog from LCDV-C,Virologica Sinica,2005,20(6):652-655
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文件名: Analysis and expression of tumor necrosis factor receptor homolog from LCDV-C.pdf
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