This paper investigated community structure, density and diversity of small-sized fish with seine in Lake Niushan, Hubei Prowince. 14 species were collected. Cultrichthys erythropterus, Taxabramis swinhonis, Pseudorasbora parva, and Acheilognathinae, including Rhodeus ocellatus, R. lighti and Paracheilognathus imberbis, were the dominant species. Majority of small-sized fishes were one year old. The density of small-sized fishes in the seine was about 5.8 ind/m(2) or 14.0 g/m(2). Diversity of fish community in the seine was low. The diversity index(H-center dot), dominance index(lambda) and evenness index(J(center dot)) of small-sized fish community were 1.802, 0.484 and 0.503 respectively. The macrophyte played an important role in determining the distribution of fish community. The biomass of small-sized fishes in the macrophyte regions was significantly higher than it in the regions without macrophyte. There was no significant effect of depth and transparence on the distribution of small-sized fish community in the Lake Niushan. Small-sized fish can be use reasonably, in order to increase economic and ecological benefit, through optimizing fish community structure and enhancing piscivorous fish.