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题名: Separation and relationships of seven Euglena strains based on microsatellite DNA fingerprinting
作者: Zhang Wenjing (zhangwenjing@ihb.ac.cn) ; Yu Yuhe (yhyu@ihb.ac.cn) ; Shen Yunfen ; Yan Qingyun
通讯作者: Yu Yuhe ; Chinese Acad Sci, Inst Hydrobiol, Lab Taxon and Ecol Protozoa, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
刊名: Hupo Kexue
发表日期: 2006
卷: 18, 期:3, 页:285-292
收录类别: 其他
英文摘要: The genetic relationships among Euglena strains remain poorly understood, despite a rich history of morphological examination and an extensive phylogenetic analysis of the ribosomal RNA genes. It is difficult to identify related species of the genus Euglena and to separate different strains of the same species because the morphological characters are variable and limited. We address this question using microsatellite DNA fingerprinting. Seven microsatellite loci were amplified from 7 Euglena strains. A total of 4-8 bands were successfully amplified for each of 6 microsatellite primer pairs. Microsatellite DNA fingerprinting and similarity values ranging from 0.000 to 0.957 among the seven strains were obtained, which were used to construct a similarity tree among them. According to the tree, at the linkage distance of 0.9346 seven strains were separated into three clusters: E. mutabilis, E. intermedia and E. gracilis consisting of Japanese and USA groups. Further, in the cluster E. gracilis, different genotypes were recognized for the strains from different geographical origins. The relationships among species and strains were analyzed preliminarily. We demonstrates that the seven strains of Euglena can be clearly separated by their microsatellite DNA fingerprinting patterns. The microsatellite DNA fingerprinting has a high resolution and is a new useful method to identify and separate similar species and intraspecific strains in free-living protozoa.
ISSN号: 1003-5427
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18359
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Zhang Wenjing (zhangwenjing@ihb.ac.cn) ; Yu Yuhe (yhyu@ihb.ac.cn) ; Shen Yunfen; Yan Qingyun.Separation and relationships of seven Euglena strains based on microsatellite DNA fingerprinting,Hupo Kexue,2006,18(3):285-292
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文件名: Separation and relationships of seven Euglena strains based on microsatellite DNA fingerprinting.pdf
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