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Establishment of cell model for the study of cytotoxicity induced by microcystin-LR
Lei La-Mei; Song Li-Rong (lrsong@ihb.ac.cn); Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
2006
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume30Issue:4Pages:408-412
AbstractMicrocystins are a group of closely related cyclic heptapeptides produced by a variety of common cyanobacteria. These toxins cause hepatocyte damage by inhibiting protein phosphastases I and 2A, resulting in hyperphosphorylation of a number of different proteins. Furthermore, high incidence of primary liver cancer in Qidong and Haimen in China has been associated with bloom contaminated drinking water. The mechanisms of MC-induced hepatotoxicity and tumor promotion activity have not been fully elucidated. In this study, Using 18 cell lines as materials, the cytotoxicity induced by different concentrations of microcystin-LR (20 and 50 mu g/mL) was studied. The morphological observation showed that five cell lines (PC-3, J82, 786-O, 5637 and VERO-E6) underwent obviously morphological changes after 30h treatment with microcystin-LR ( MCLR) with a dose-dependent manner. The cytoxicity of MCLR was determined by LDH leakage that showed a dose-dependent increase in five cell lines treated with MCLR, of which LDH leakage for 5637 and PC-3 were more serious under the same treatment. The SOD activity for five cell lines tended to increase in 20 mu g/mL MCLR treatment but sharply decrease in 50 mu g/mL MCLR treatment compared with the control cells; the GSH content decreased totally in different treatment. Based on the analysis of the sensitivity discrepancy for MCLR, 5637 cell lines was chosen for the establishment of a cell model for the study of cytotoxicity induced by microcystin-LR.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18343
Collection期刊论文
Corresponding AuthorLei La-Mei; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Lei La-Mei,Song Li-Rong ,Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China. Establishment of cell model for the study of cytotoxicity induced by microcystin-LR[J]. Acta Hydrobiologica Sinica,2006,30(4):408-412.
APA Lei La-Mei,Song Li-Rong ,&Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China.(2006).Establishment of cell model for the study of cytotoxicity induced by microcystin-LR.Acta Hydrobiologica Sinica,30(4),408-412.
MLA Lei La-Mei,et al."Establishment of cell model for the study of cytotoxicity induced by microcystin-LR".Acta Hydrobiologica Sinica 30.4(2006):408-412.
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