IHB OpenIR  > 期刊论文
Studies on s110886, a gene essential to light-activated heterotrophic growth in Synechocystis sp PCC6803
Liu Zhao-Ying; Xu Xu-Dong; Kong Ren-Qiu (kongr@ihb.ac.cn); Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2006
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume30Issue:4Pages:375-379
AbstractSynechocystis sp. PCC6803 is able to grow on glucose in complete darkness with a daily stimulation of short and weak light, which is called light activated heterotrophic growth (LAHG). By random insertion of a kanamycin-resistance cassette into Hae III or Alu I sites of the genome, a mutant library of Synechocystis sp. PCC6803 was constructed. From this library, 3 mutants incapable of LAHG but similar to wild type in autotrophic, mixotrophic and phototrophic growth were selected. Inverse PCR and sequence determination showed that all the insertion were at a Hae III (GGCC,666-669 Nt.) site of s110886. s110886 without its N-terminal trans-membrane encoding sequence was generated by PCR, cloned into pET21b and expressed in E. coli BL21 ( DE3). The expressed product, found in the soluble fraction, was purified with the assistance of its C-terminal His. Tag and used to prepare the rabbit antiserum against S110886. Western Blot was employed to investigate the presence of SI 10886 in membrane or soluble protein fraction of the cyanobaeterium, and it was found to be a membrane protein. Using total membrane from cells with different periods of light exposure, the expression of s110886 was examined and found to be non-responsive to illumination. We conclude that s110886 is essential to LAHG and not induced by light.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18339
Collection期刊论文
Corresponding AuthorLiu Zhao-Ying; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu Zhao-Ying,Xu Xu-Dong,Kong Ren-Qiu ,et al. Studies on s110886, a gene essential to light-activated heterotrophic growth in Synechocystis sp PCC6803[J]. Acta Hydrobiologica Sinica,2006,30(4):375-379.
APA Liu Zhao-Ying,Xu Xu-Dong,Kong Ren-Qiu ,&Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2006).Studies on s110886, a gene essential to light-activated heterotrophic growth in Synechocystis sp PCC6803.Acta Hydrobiologica Sinica,30(4),375-379.
MLA Liu Zhao-Ying,et al."Studies on s110886, a gene essential to light-activated heterotrophic growth in Synechocystis sp PCC6803".Acta Hydrobiologica Sinica 30.4(2006):375-379.
Files in This Item:
File Name/Size DocType Version Access License
Studies on s110886, (684KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu Zhao-Ying]'s Articles
[Xu Xu-Dong]'s Articles
[Kong Ren-Qiu (kongr@ihb.ac.cn)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu Zhao-Ying]'s Articles
[Xu Xu-Dong]'s Articles
[Kong Ren-Qiu (kongr@ihb.ac.cn)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu Zhao-Ying]'s Articles
[Xu Xu-Dong]'s Articles
[Kong Ren-Qiu (kongr@ihb.ac.cn)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Studies on s110886, a gene essential to light-activated heterotrophic growth in Synechocystis sp PCC6803.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.