中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: Studies on s110886, a gene essential to light-activated heterotrophic growth in Synechocystis sp PCC6803
作者: Liu Zhao-Ying ; Xu Xu-Dong ; Kong Ren-Qiu (kongr@ihb.ac.cn)
通讯作者: Liu Zhao-Ying ; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
刊名: Acta Hydrobiologica Sinica
发表日期: 2006
卷: 30, 期:4, 页:375-379
收录类别: 其他
英文摘要: Synechocystis sp. PCC6803 is able to grow on glucose in complete darkness with a daily stimulation of short and weak light, which is called light activated heterotrophic growth (LAHG). By random insertion of a kanamycin-resistance cassette into Hae III or Alu I sites of the genome, a mutant library of Synechocystis sp. PCC6803 was constructed. From this library, 3 mutants incapable of LAHG but similar to wild type in autotrophic, mixotrophic and phototrophic growth were selected. Inverse PCR and sequence determination showed that all the insertion were at a Hae III (GGCC,666-669 Nt.) site of s110886. s110886 without its N-terminal trans-membrane encoding sequence was generated by PCR, cloned into pET21b and expressed in E. coli BL21 ( DE3). The expressed product, found in the soluble fraction, was purified with the assistance of its C-terminal His. Tag and used to prepare the rabbit antiserum against S110886. Western Blot was employed to investigate the presence of SI 10886 in membrane or soluble protein fraction of the cyanobaeterium, and it was found to be a membrane protein. Using total membrane from cells with different periods of light exposure, the expression of s110886 was examined and found to be non-responsive to illumination. We conclude that s110886 is essential to LAHG and not induced by light.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18339
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Studies on s110886, a gene essential to light-activated heterotrophic growth in Synechocystis sp PCC6803.pdf(684KB)----开放获取--View Download

Recommended Citation:
Liu Zhao-Ying; Xu Xu-Dong; Kong Ren-Qiu (kongr@ihb.ac.cn).Studies on s110886, a gene essential to light-activated heterotrophic growth in Synechocystis sp PCC6803,Acta Hydrobiologica Sinica,2006,30(4):375-379
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu Zhao-Ying]'s Articles
[Xu Xu-Dong]'s Articles
[Kong Ren-Qiu (kongr@ihb.ac.cn)]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu Zhao-Ying]‘s Articles
[Xu Xu-Dong]‘s Articles
[Kong Ren-Qiu (kongr@ihb.ac.cn)]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Studies on s110886, a gene essential to light-activated heterotrophic growth in Synechocystis sp PCC6803.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace