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题名: Genome size determination of Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis
作者: Du Bo ; Wang Ding (wangd@ihb.ac.cn) ; Zhang Xian-Feng ; Guo Zheng ; Zhang Jing
通讯作者: Wang Ding ; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
刊名: Acta Zoologica Sinica
发表日期: 2006
卷: 52, 期:4, 页:731-737
收录类别: 其他
英文摘要: The present study determined C-values of 23 individuals of Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis by flow cytometry using chicken erythrocyte DNA content as a standard. The Yangtze finless porpoise's lymphocyte cells were extracted from whole blood samples stored at three different conditions. For determining the actual C-value of chicken erythrocyte, we firstly standardized its C-value controlled by human C-value of 3.50 pg/C, and then the chicken C-value of 2.35 pg/2C was used in the following determinations. Result shows that the C-value of the Yangtze finless porpoise is 3.27 pg with the genome size of 3.17 x 10(9) bp. The C-values for male and female are 3.29 pg and 3.25 pg, respectively. The C-values of Yangtze finless porpoises reared in captivity and wild are 3.06 pg and 3.30 pg, respectively. Analyzed by Independent-Samples T Test, it's found that the C-values of different genders have no significant difference, whereas, the C-values of wild Yangtze finless porpoises and that reared in captivity have significant differences, the C-value of Yangtze finless porpoise in captivity is smaller than that of the wild ones. It's assumed that the stability of environmental factors has certain influences on animal genome size variation.
ISSN号: 0001-7302
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18335
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Du Bo; Wang Ding (wangd@ihb.ac.cn) ; Zhang Xian-Feng; Guo Zheng; Zhang Jing.Genome size determination of Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis,Acta Zoologica Sinica,2006,52(4):731-737
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文件名: Genome size determination of Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis.pdf
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