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题名: Construction of a phage display random decapeptide library and panning for mimic antigen epitope of ScFv A1 against WSSV
作者: Yuan Li ; Wang Yu-Zhen ; Xiao Nan ; Zhang Xiao-hua ; Dai He-ping (hpdai@ihb.ac.cn)
通讯作者: Dai He-ping ; Wuhan Univ, Inst Hydrobiol, Wuhan 430072, Peoples R China
刊名: Virologica Sinica
发表日期: 2006
卷: 21, 期:6, 页:614-618
收录类别: 其他
英文摘要: A phage display random decapeptide library with high titer was constructed using pCANTAB 5 E as vector and used to select and identify antigen epitopes. ScFv Al, which specifically binds the shrimp's White spot syndrome virus (WSSV), was used to pan against the decapeptide library and a random 15mer library, respectively. A series of positive clones that bind specifically to scFv Al were obtained. The amino acid sequences of the positive peptides were compared with WSSV388 fragment, which is the antigen of scFv Al. We found that most of the sequences of the positive peptides were similar to a fragment, K center dot center dot center dot center dot R center dot center dot R center dot Q, located on C-terminal of WSSV388 fragment. We deduced that the mimic antigen epitopes of scFv Al were conformational epitopes structured by the discontinuous amino acid fragment, but not by a linear amino acid fragment. These results show that phage display peptide library technology is a powerful method for analyzing the structure of antigen epitopes, which is helpful to further explore conformation and function of the structural proteins of WSSV, and the mechanisms of interactions of the virus with the antiviral antibodies and host cells.
ISSN号: 1674-0769
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18317
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Recommended Citation:
Yuan Li; Wang Yu-Zhen; Xiao Nan; Zhang Xiao-hua; Dai He-ping (hpdai@ihb.ac.cn).Construction of a phage display random decapeptide library and panning for mimic antigen epitope of ScFv A1 against WSSV,Virologica Sinica,2006,21(6):614-618
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文件名: Construction of a phage display random decapeptide library and panning for mimic antigen epitope of ScFv A1 against WSSV.pdf
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