IHB OpenIR  > 期刊论文
Construction of a phage display random decapeptide library and panning for mimic antigen epitope of ScFv A1 against WSSV
Yuan Li; Wang Yu-Zhen; Xiao Nan; Zhang Xiao-hua; Dai He-ping (hpdai@ihb.ac.cn); Dai He-ping; Wuhan Univ, Inst Hydrobiol, Wuhan 430072, Peoples R China
2006
Source PublicationVirologica Sinica
ISSN1674-0769
Volume21Issue:6Pages:614-618
AbstractA phage display random decapeptide library with high titer was constructed using pCANTAB 5 E as vector and used to select and identify antigen epitopes. ScFv Al, which specifically binds the shrimp's White spot syndrome virus (WSSV), was used to pan against the decapeptide library and a random 15mer library, respectively. A series of positive clones that bind specifically to scFv Al were obtained. The amino acid sequences of the positive peptides were compared with WSSV388 fragment, which is the antigen of scFv Al. We found that most of the sequences of the positive peptides were similar to a fragment, K center dot center dot center dot center dot R center dot center dot R center dot Q, located on C-terminal of WSSV388 fragment. We deduced that the mimic antigen epitopes of scFv Al were conformational epitopes structured by the discontinuous amino acid fragment, but not by a linear amino acid fragment. These results show that phage display peptide library technology is a powerful method for analyzing the structure of antigen epitopes, which is helpful to further explore conformation and function of the structural proteins of WSSV, and the mechanisms of interactions of the virus with the antiviral antibodies and host cells.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18317
Collection期刊论文
Corresponding AuthorDai He-ping; Wuhan Univ, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Yuan Li,Wang Yu-Zhen,Xiao Nan,et al. Construction of a phage display random decapeptide library and panning for mimic antigen epitope of ScFv A1 against WSSV[J]. Virologica Sinica,2006,21(6):614-618.
APA Yuan Li.,Wang Yu-Zhen.,Xiao Nan.,Zhang Xiao-hua.,Dai He-ping .,...&Wuhan Univ, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2006).Construction of a phage display random decapeptide library and panning for mimic antigen epitope of ScFv A1 against WSSV.Virologica Sinica,21(6),614-618.
MLA Yuan Li,et al."Construction of a phage display random decapeptide library and panning for mimic antigen epitope of ScFv A1 against WSSV".Virologica Sinica 21.6(2006):614-618.
Files in This Item: Download All
File Name/Size DocType Version Access License
Construction of a ph(316KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yuan Li]'s Articles
[Wang Yu-Zhen]'s Articles
[Xiao Nan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yuan Li]'s Articles
[Wang Yu-Zhen]'s Articles
[Xiao Nan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yuan Li]'s Articles
[Wang Yu-Zhen]'s Articles
[Xiao Nan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Construction of a phage display random decapeptide library and panning for mimic antigen epitope of ScFv A1 against WSSV.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.