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Construction of copper-induced gene expression platform in Synechocystis sp pcc6803
Gao Hong; Tang Qing; Xij Xu-Dong; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
2007
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume31Issue:2Pages:240-244
AbstractIn Synechocystis sp. PCC6803, gene knock-out is the most straightforward and effective method to reveal the physiological function of a gene. Nevertheless, insertion mutants could not be generated for those genes essential to survival. In order V to elucidate the function of such genes in Synechocystis sp. PCC6803, a copper-induced gene expression platform was constructed using the promoter of petE (PpetE). PpetE from Synechocystis sp. PCC6803 and the beta-galactosidase gene (lacZ) from E. coli GM48 were cloned respectively by doing polymerase chain reaction (PCR), and the PpetE was positioned upstream of lacZ. The PpetE-lacZ construct was integrated into the genome of Synechocystis sp. PCC6803 via homologous recombinations. The expression of beta-galactosidase gene was found to be controllable by adjusting the concentration Of CU2+ in medium. In a range from 6 to 400nmol/L, Cu2+ induced the expression of beta-galactosidase in an S-shaped curve, but when the concentration of cu(2+) in medium was below 6nmol/L or above 400nmol/L, the activity of beta-galactosidase was either too low to be detected or too high to be regulated. This copper-induced gene expression platform can be used in the control of some indispensable genes in Synechocystis sp. PCC6803 : eel Is survived in the presence of Cu2+ so that the physiological effect of a gene Could be observed when Cu2+ is removed.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18275
Collection期刊论文
Corresponding AuthorGao Hong; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Gao Hong,Tang Qing,Xij Xu-Dong,et al. Construction of copper-induced gene expression platform in Synechocystis sp pcc6803[J]. Acta Hydrobiologica Sinica,2007,31(2):240-244.
APA Gao Hong,Tang Qing,Xij Xu-Dong,&Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China.(2007).Construction of copper-induced gene expression platform in Synechocystis sp pcc6803.Acta Hydrobiologica Sinica,31(2),240-244.
MLA Gao Hong,et al."Construction of copper-induced gene expression platform in Synechocystis sp pcc6803".Acta Hydrobiologica Sinica 31.2(2007):240-244.
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