中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: Temporal and spatial expression pattern of gibel carp pou2 gene during embryogenesis
作者: Yin Jun (yinjun@ihb.ac.cn) ; Zhou Li ; Liu Jun ; Du Xin-Zheng ; Gui Jian-Fang (jfgui@ihb.ac.cn)
通讯作者: Yin Jun ; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol and Biotechnol, Wuhan 430072, Peoples R China
刊名: Acta Hydrobiologica Sinica
发表日期: 2007
卷: 31, 期:5, 页:629-636
收录类别: 其他
英文摘要: Full-length cDNA of Gibel carp pou2 gene was cloned front gastrula SMART cDNA library through RACE-PCR. It consists of 2421 bp, with an ORF of 1416 bp encoding a protein of 471 aa. The complete amino acid sequence shares 91.0% identity with zebrafish pou2 gene product. Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result. indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed front blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells. In blastula embryos and 50% epiboly erubrvos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain and hindbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result. The expression pattern of gibel carp pou2 suggests that this gene should play an important role during early stages of embryogenesis. It might regulate the formation of neural plate and the fate of midbrain and hindbrain cells during early embryogenesis.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18209
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Temporal and spatial expression pattern of gibel carp pou2 gene during embryogenesis.pdf(1155KB)----开放获取--View Download

Recommended Citation:
Yin Jun (yinjun@ihb.ac.cn) ; Zhou Li; Liu Jun; Du Xin-Zheng; Gui Jian-Fang (jfgui@ihb.ac.cn).Temporal and spatial expression pattern of gibel carp pou2 gene during embryogenesis,Acta Hydrobiologica Sinica,2007,31(5):629-636
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yin Jun (yinjun@ihb.ac.cn)]'s Articles
[Zhou Li]'s Articles
[Liu Jun]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yin Jun (yinjun@ihb.ac.cn)]‘s Articles
[Zhou Li]‘s Articles
[Liu Jun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Temporal and spatial expression pattern of gibel carp pou2 gene during embryogenesis.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace