Full-length cDNA of Gibel carp pou2 gene was cloned front gastrula SMART cDNA library through RACE-PCR. It consists of 2421 bp, with an ORF of 1416 bp encoding a protein of 471 aa. The complete amino acid sequence shares 91.0% identity with zebrafish pou2 gene product. Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result. indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed front blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells. In blastula embryos and 50% epiboly erubrvos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain and hindbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result. The expression pattern of gibel carp pou2 suggests that this gene should play an important role during early stages of embryogenesis. It might regulate the formation of neural plate and the fate of midbrain and hindbrain cells during early embryogenesis.
Yin Jun (firstname.lastname@example.org) ; Zhou Li; Liu Jun; Du Xin-Zheng; Gui Jian-Fang (email@example.com).Temporal and spatial expression pattern of gibel carp pou2 gene during embryogenesis,Acta Hydrobiologica Sinica,2007,31(5):629-636