中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: Whole-ceilPCR to identify Anabaena species and strains
作者: Huang Jia-Quan ; Wang Gao-Hong ; Li Dun-Hai ; Liu Yong-ding (liuyd@ihb.ac.cn) ; Yin Li-Yan
通讯作者: Huang Jia-Quan ; Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China
刊名: Acta Hydrobiologica Sinica
发表日期: 2004
卷: 28, 期:2, 页:159-162
收录类别: 其他
英文摘要: The traditional way to identify algae is usually depend on the observation of the morphology. This way is time consuming and usually difficult to identify the algae during the early algal growth stage in which the character is not obvious and can not detect the subtle differences between species or strains; also, the morphology and the ability to produce toxicants of cyanobacteria may change under different natural or cultural conditions. In this paper, we use the molecular method to identify Anabaena species, the results showed that BSA are necessary for the PCR amplification and the optimal concentration is 1% (w/v) in the reacion buffer. DMSO increased or decreased the quantity of the amplification products selectively, but the effect is not significant. The self designed primers after comparison of other Anabaena PC sequences amplified the part of pcb and pca gene along with PC-IGS sequence when using both extracted DNA and pretreated whole cells as template, the banding pattern is not uniform but all produced the target bands, cloning and sequencing analyses indicated that PC in line with PC-IGS was amplified, even though the nucleotide between two Anabaena strains collected from two different regions in China show that only 2 sites in a fragment about 400 bp are different, but the PC-IGS sequences are quite different from others and thus can be applied to identify the different species or strains. The universal primers to amplify part of 16S rDNA and 23S rDNA along with their ITS sequence using the extracted DNA and pretreated whole cell showed that the banding pattern of amplification products are uniform when the whole cells were at the concentration of 105/mL in the reaction buffer, the minimum amount of whole cell to amplify the target band is 20 cells in 50muL reaction buffer.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18133
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Whole-ceilPCR to identify Anabaena species and strains.pdf(210KB)----开放获取--View Download

Recommended Citation:
Huang Jia-Quan; Wang Gao-Hong; Li Dun-Hai; Liu Yong-ding (liuyd@ihb.ac.cn) ; Yin Li-Yan.Whole-ceilPCR to identify Anabaena species and strains,Acta Hydrobiologica Sinica,2004,28(2):159-162
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Huang Jia-Quan]'s Articles
[Wang Gao-Hong]'s Articles
[Li Dun-Hai]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Huang Jia-Quan]‘s Articles
[Wang Gao-Hong]‘s Articles
[Li Dun-Hai]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Whole-ceilPCR to identify Anabaena species and strains.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace