中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: The effect of microcystin on some physio-biochemical characteristics of Synechococcus elongatus
作者: Hu Zhi-Quan ; Liu Yong-ding (liuyd@ihb.ac.cn)
通讯作者: Hu Zhi-Quan ; Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China
刊名: Acta Hydrobiologica Sinica
发表日期: 2004
卷: 28, 期:2, 页:155-158
收录类别: 其他
英文摘要: In China, lake eutrophication is serious. Many lakes are close to death because receiving too big amounts of polluted water. Recently, heavy blooms frequently occured in Dianchi Lake and Microcystis is the dominant species in the bloom, and in many cases, when appeared in the lake, forms a continuous bloom all along the year, eliminating quite all the other species. Microcystis produces a family of related cyclic hepatopeptides ( microcystins, MC). These toxins are severely hepatotoxic, produced in Microcystis cells and released into water body when algal cells were broken. Most investigations about the toxicity of the microcystins are focused on animals and higher plants. To our knowledge, few studies have been made on the possible effects of microcystins on algae, which are equally important as primary producers in the whole ecosystem. In this study, We chose the unicellular Synechococcus elongatus (one of the most studied, and geographically most distributed, cyanobacteria in the picoplankton) as test material and investigated the toxicological effects of MC-RR on it. For this purpose, some physio-biochemical parameters (cell optical density, chlorophyll fluorescence( Fv/Fm), pigment content( chlorophyll, phycocyanin, soluable protein content, soluable and insoluable carbohydrate content) were tested in algal cells when exposed to 100mug/L microcystin-RR. Microcstin-RR was isolated and purified with the high performance liquid chromatography( HPLC) method. Thereafter, known amount of purified toxin in distilled water was added to test culture flasks in the way to obtain a final toxin concentration in cultures of 100mug.L-1. The results showed that the growth of Synechococcus elongatus (shown as optical density) was significantly inhibited compared with the controls. After exposure to the toxin, the OD665 were lower than those of the controls from (lay 2 to day 10. The OD665 of the control kept in a increasing tendency but that of the toxin-treated decreased on day 8, which meant that the algae had came into death phase in advance. At the same time, a difference in chlorophyll flurorescence(the efficiency of excitation capture by open photo system U (PS U)reaction centers, expressed as Fv/Fm) also appeared. Fv/Fm of the controls kept at a stable high level. In constrast, that of the toxin-treated algae decreased quickly from day 4. The results also demonstrated that toxin treatment could cause the changes of some biochemical characters. For example, the content of soluable protein and soluable, insoluable carbohydrate all reduced after toxin exposure. Alough the content of chlorophyll and phycocyanin of the toxin-treated algae decreased, the ratio of PC/Chl was higher than that of the control. From th present study we could infer the possible toxicological mechanism of microcystin-RR on the cyanobacterium Synechococcus elongatus. Microcystin could inhibit the photosynthetic ability and the inhibition may cause the lack of nutrition( protein, carbohydrate) the decrease of the biochemical reactions and the cell division rate. As a result the growth of the algae was significantly influenced. These results also suggested that microcystins could cause lethal effect on competitive algae and microcystins maybe play a role in phytoplankton succession and population regulation.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18131
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
The effect of microcystin on some physio-biochemical characteristics of Synechococcus elongatus.pdf(158KB)----开放获取--View Download

Recommended Citation:
Hu Zhi-Quan; Liu Yong-ding (liuyd@ihb.ac.cn).The effect of microcystin on some physio-biochemical characteristics of Synechococcus elongatus,Acta Hydrobiologica Sinica,2004,28(2):155-158
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Hu Zhi-Quan]'s Articles
[Liu Yong-ding (liuyd@ihb.ac.cn)]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Hu Zhi-Quan]‘s Articles
[Liu Yong-ding (liuyd@ihb.ac.cn)]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: The effect of microcystin on some physio-biochemical characteristics of Synechococcus elongatus.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace