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Preliminary study on applicability of microsatellite DNA primers from parasiteprotozoa Trypanosoma cruzi in free-living protozoa
Zhang Wenjing; Yu Yuhe (yhyu@ihb.ac.cn); Shen Yunfen; Miao Wei; Feng Weisong; Yu Yuhe; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2004
Source PublicationJournal of Ocean University of China
ISSN1672-5182
Volume3Issue:1Pages:80-84
AbstractIn this paper, we took the lead in studying on specificity of the microsatellite DNA loci and applicability of microsatellite DNA primers in protozoa. In order to study characters of microsatellites in free-living protozoa, eight microsatellite loci primers developed from Trypanosoma cruzi (MCLE01, SCLE10, MCLE08, SCLE11, MCLF10, MCLG10, MCL03, MCL05) were employed to amplify microsatellite in four free-living protozoa, including Bodo designis, Euglena gracilis FACHB848, Paramecium bruzise and Tetrahymena thermophila BF1. In the amplification systems of P. bruzise, four loci (SCLE10, SCLE11, MCLF10, MCL03) were amplified successfully, and four amplification fragments were in proper size. In genome of E. gracilis FACHB848, five of eight primers brought five clear amplification bands. In B. designis, three (No. 4, 5 and 7) of eight loci produced clear and sharp products without stutter bands, whereas no bands appeared in T. thermophila BF1. Further, eight 300-500bp amplification fragments were cloned and sequenced. Nevertheless, all sequenced products did not contain corresponding microsatellite sequence, although Bodo is in the same order and has the nearest phylogenetic relation with Trypanosoma among these four species. Thus, the microsatellite DNA primers can not be applied among order or more far taxa, and the specificity of microsatellite DNA is very high in protozoa. The results of this study will contribute to our understanding of microsatellite DNA in protozoa.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18107
Collection期刊论文
Corresponding AuthorYu Yuhe; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang Wenjing,Yu Yuhe ,Shen Yunfen,et al. Preliminary study on applicability of microsatellite DNA primers from parasiteprotozoa Trypanosoma cruzi in free-living protozoa[J]. Journal of Ocean University of China,2004,3(1):80-84.
APA Zhang Wenjing.,Yu Yuhe .,Shen Yunfen.,Miao Wei.,Feng Weisong.,...&Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2004).Preliminary study on applicability of microsatellite DNA primers from parasiteprotozoa Trypanosoma cruzi in free-living protozoa.Journal of Ocean University of China,3(1),80-84.
MLA Zhang Wenjing,et al."Preliminary study on applicability of microsatellite DNA primers from parasiteprotozoa Trypanosoma cruzi in free-living protozoa".Journal of Ocean University of China 3.1(2004):80-84.
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