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题名: Preliminary study on applicability of microsatellite DNA primers from parasiteprotozoa Trypanosoma cruzi in free-living protozoa
作者: Zhang Wenjing ; Yu Yuhe (yhyu@ihb.ac.cn) ; Shen Yunfen ; Miao Wei ; Feng Weisong
通讯作者: Yu Yuhe ; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
刊名: Journal of Ocean University of China
发表日期: 2004
卷: 3, 期:1, 页:80-84
收录类别: 其他
英文摘要: In this paper, we took the lead in studying on specificity of the microsatellite DNA loci and applicability of microsatellite DNA primers in protozoa. In order to study characters of microsatellites in free-living protozoa, eight microsatellite loci primers developed from Trypanosoma cruzi (MCLE01, SCLE10, MCLE08, SCLE11, MCLF10, MCLG10, MCL03, MCL05) were employed to amplify microsatellite in four free-living protozoa, including Bodo designis, Euglena gracilis FACHB848, Paramecium bruzise and Tetrahymena thermophila BF1. In the amplification systems of P. bruzise, four loci (SCLE10, SCLE11, MCLF10, MCL03) were amplified successfully, and four amplification fragments were in proper size. In genome of E. gracilis FACHB848, five of eight primers brought five clear amplification bands. In B. designis, three (No. 4, 5 and 7) of eight loci produced clear and sharp products without stutter bands, whereas no bands appeared in T. thermophila BF1. Further, eight 300-500bp amplification fragments were cloned and sequenced. Nevertheless, all sequenced products did not contain corresponding microsatellite sequence, although Bodo is in the same order and has the nearest phylogenetic relation with Trypanosoma among these four species. Thus, the microsatellite DNA primers can not be applied among order or more far taxa, and the specificity of microsatellite DNA is very high in protozoa. The results of this study will contribute to our understanding of microsatellite DNA in protozoa.
ISSN号: 1672-5182
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18107
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Zhang Wenjing; Yu Yuhe (yhyu@ihb.ac.cn) ; Shen Yunfen; Miao Wei; Feng Weisong.Preliminary study on applicability of microsatellite DNA primers from parasiteprotozoa Trypanosoma cruzi in free-living protozoa,Journal of Ocean University of China,2004,3(1):80-84
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文件名: Preliminary study on applicability of microsatellite DNA primers from parasiteprotozoa Trypanosoma cruzi in free-living protozoa.pdf
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